Dissertation/Thèse
2024
Thèses
1
  • DAYANA FERREIRA FERNANDES
  • TOXICOLOGICAL EVALUATION OF THE DELETORY EFFECTS OF THE BENZODIAZEPINES: CLONAZEPAM AND ALPRAZOLAM ON THE INITIAL DEVELOPMENT OF ZEBRAFISH (Danio rerio)

  • Leader : HELIO BATISTA DOS SANTOS
  • MEMBRES DE LA BANQUE :
  • AMANDA LUISA DA FONSECA
  • ANA GABRIELA SILVA OLIVEIRA
  • ANDERSON KELVIN SARAIVA MACÊDO
  • HELIO BATISTA DOS SANTOS
  • LEANDRO AUGUSTO DE OLIVEIRA BARBOSA
  • RALPH GRUPPI THOME
  • Data: 26 mars 2024


  • Afficher le Résumé
  • Introduction: Pharmaceutical products have been observed in the environment, and environmental contamination by these emerging pollutants can occur in several ways, which can consequently cause serious ecological consequences for non-target organisms and even human health. Objectives: To evaluate the toxicological effects individually and as a cocktail of benzodiazepines (BZDs), clonazepam (CLO) and alprazolam (ALP) during the early development of zebrafish (Danio rerio). Material and methods: To evaluate the toxicological effects of BZDs during early embryonic development of zebrafish, Fish Embryo Toxicity (FET) was used. The lethal concentrations that promote 50% of the death of zebrafish embryos (LC50) were calculated, the fish were subjected to the following concentrations: ALP (0.125; 0.25; 0.50; 1.0 and 3.0 mg/ L) and CLO (20; 50; 100; 120 and 150 mg/L). After determining the LC50, an embryotoxic assay was carried out using concentrations of ALP and CLO alone or in a cocktail, to evaluate the lethal (mortality) and sublethal effects (heart beat per minute (bpm), hypoactivity, total length and morphological abnormalities). Results: An LC50 of 2 mg/L for ALP and 95 mg/L for CLO were found for zebrafish embryos exposed to increasing concentrations of BZDs during FET. In the embryotoxic assay with BZDs, a delay in hatching of embryos with the cocktail at higher concentrations was observed compared to the control group, as well as greater mortality after hatching. Embryos exposed to concentrations of ALP and CLO showed significant mortality results (p < 0.05) when compared to the control group. Toxicity was greater with increasing drug concentration in individual experiments, especially in mixtures. The embryos subjected to BZDs, in isolation and in the cocktail, showed anatomical abnormalities such as pericardial and yolk sac edema and spinal and tail malformations and depigmentation. Conclusion: In this present work, it was possible to observe toxic effects arising from the exposure of BZDs, both individually and in the form of an exposure cocktail during the initial development of D. rerio. Exposure to BZDs, especially at higher concentrations, promoted several morphological changes in zebrafish, and the appearance of anatomical malformations can directly affect their survival in the environment. Therefore, BZDs released inappropriately into the environment have a potential toxic effect on aquatic life and human health.

2023
Thèses
1
  • JICAURY ROBERTA PEREIRA DA SILVA
  • EVALUATION OF THE TOXICITY OF PLASTIFYERS USED AS DRUGS EXCIPIENTS, USING FISH AS AN EXPERIMENTAL MODEL

  • Leader : HELIO BATISTA DOS SANTOS
  • MEMBRES DE LA BANQUE :
  • CAMILA FERREIRA SALES
  • HELIO BATISTA DOS SANTOS
  • LUCIANA XAVIER PEREIRA
  • LUDMILA SILVA BRIGHENTI
  • WHOCELY VICTOR DE CASTRO
  • Data: 16 févr. 2023


  • Afficher le Résumé
  • The widespread production and use of medicines by the population results in greater
    observation of pharmaceutical products and their derivatives in aquatic environments.
    Certain plastic additives used in the coating of dosage forms can cause harm to the
    patient's health and the environment. Among plasticizers, phthalate esters (PAEs),
    derived from phthalic acid (C6H4(COOH)2), have been widely investigated for their
    toxicological potential. PAEs have the ability to adversely interact with various biological
    processes, such as growth, metabolism, endocrine and immune systems. The present
    study aims to identify the toxicological effects of low molecular weight phthalates such
    as diethylphthalate (DEP) and dibutylphthalate (DBP) using the Fish Embryo Acute
    Toxicity (FET) technique whose animal model is the Danio rerio (zebrafish). Embryos
    were exposed to concentrations of 0.70- 0.35- 0.17- 0.08 mg/L of DBP and 24- 12- 6- 3
    mg/L of DEP, and evaluated at times of 24, 48, 72 and 96 hours post fertilization (hpf)
    for identification of toxic effects. The hatching rate and embryonic mortality were
    evaluated, as well as the appearance and number of somites, the number of heartbeats per
    minute and length, in addition to anatomical abnormalities (teratogenic effects) such as
    malformation of the head, spine and tail. After exposure to PAEs, embryos exposed to
    higher concentrations of DBP and DEP showed an increase in the number of somites,
    irregular heartbeat and higher heart rate when compared to the control group. DBP more
    frequently induced pericardial edema in the embryos, whereas DEP had a greater effect
    on the hatching rate, and induced a higher rate of malformations in the tail, at the highest
    concentration (24mg/L) 75% of the embryos showed necrosis or crooked tail. DBP is
    more toxic than DEP as it induces adverse effects on embryos at considerably lower
    concentrations. Phthalate contamination is a public health issue and needs to be given due
    attention. In this way, we hope that the results obtained can enable the understanding and
    extrapolate to nature the damage caused to living beings due to exposure to these
    xenobiotics that are released into the environment.
2
  • Wellington Fernandes de Carvalho
  • EVALUATION OF THE COCKTAIL EFFECT OF DIPYRONE, PARACETAMOL AND NIMESULIDE ON THE INITIAL DEVELOPMENT OF ZEBRAFISH (Danio rerio)

  • Leader : HELIO BATISTA DOS SANTOS
  • MEMBRES DE LA BANQUE :
  • AMANDA LUISA DA FONSECA
  • FABRICIO FLÁVIO THEOPHILO DOMINGOS
  • HELIO BATISTA DOS SANTOS
  • Data: 30 mars 2023


  • Afficher le Résumé
  • Pharmaceutical products, especially medicines, are of great importance to society. Growing consumption and inappropriate disposal are inserting these products into a new class of xenobiotics distributed as emerging pollutants, becoming a major public health concern. Fish, in particular the embryonic development of zebrafish, have been widely used in environmental toxicology research. Most works using fish have been focused mainly on the toxicological evaluation of isolated drugs, however, the effects of the mixture (cocktail) of these xenobiotics have been little explored and this is the possible scenario found in the environment. A previous study indicated that the most incorrectly discarded drugs by users of drugstores in the city of Divinópolis, MG were dipyrone, paracetamol and nimesulide. In this sense, our objective was to evaluate the cocktail effect of dipyrone, paracetamol and nimesulide during the embryonic development of zebrafish. The lethal concentration of 50% of the embryos at 96 h (CL 50-96 h) for the drugs of interest was determined. After this step, the following experimental groups were associated with the mixture: Control (E3 medium), CL 50/2, CL 50/5, CL 50/10 and CL 50/20. Embryos with 6-8 hours post fertilization (hpf) were submitted to the treatment and the following times were evaluated: 24, 48, 72 and 96 h. In the morphometric evaluation of the embryos, the number of heart beats, hatching rate and embryo mortality by development time, as well as the total length, size of the heart area and yolk sac edema were obtained. In addition, teratogenic effects have been verified. The LC 50/96 h calculated for dipyrone, paracetamol and nimesulide were, respectively, 4.69 mg/ml, 799.98 μg/ml and 0.92 μg/ml. After the toxicological tests, it was possible to observe that the exposure of the embryos to the mixture induced a significant increase in the mortality rate and a decrease in the hatching rate, being more frequent in the CL 50/5 and CL 50/2 concentrations, in addition, teratogenic effects were observed in the embryos, such as malformation of the head, curvature of the tail and spine, vitelline and pericardial edema and loss of pigmentation, with the most intense depigmentation in the eyeballs and dorsal region of the larvae, mainly in CL 50/5 and CL 50/2 concentrations . Finally, we believe that the present work, based on the results obtained, will make it possible to understand and extrapolate to nature the damage caused to living beings due to the accidental exposure of these xenobiotics released into the environment.

3
  • Jaqueline Campos Costa
  • CONTROLLED CLINICAL STUDIES ENVOLVING THE USE OF PHYTOTHERAPY OR PLANT DERIVATIVES IN WOUND HEALING: A SYSTEMATIC REVIEW

  • Leader : CARLOS EDUARDO DE MATOS JENSEN
  • MEMBRES DE LA BANQUE :
  • ANA LUIZA CHAVES MAIA
  • CARLOS EDUARDO DE MATOS JENSEN
  • ELIANA DE FARIA GARCIA HORTA
  • MAGNA CRISTINA DE PAIVA
  • MARCELO GONZAGA DE FREITAS ARAUJO
  • Data: 31 mars 2023


  • Afficher le Résumé
  • Introduction: The use of phytotherapy has increased considerably because it provides the population with an effective alternative to access health. The trend is for phytotherapy to participate progressively and more intensely in health care due to the significant therapeutic effects achieved. Such effects must be proven by clinical studies, meeting scientific and ethical standards. Therefore, after carrying out these clinical studies, with proof of their effectiveness, the population will be more confident in the use of these pharmaceutical products. This encourages not only the use of these herbal medicines and medicinal plants by the general population, but also their prescription by health professionals. Objective(s): To evaluate which herbal medicines already have proven clinical efficacy for the treatment of skin lesions. Methodology: The search was performed in five different databases: Scopus, Web of Science, Science Direct, Pubmed and Virtual Health Library (VHL) in June, 2021. Different Mesh descriptors were combined using a Boolean operator in the English language. The search and elaboration of this review followed the guidelines proposed by the PRISMA recommendation. Perspective: It is expected that the data on herbal medicines used to cure skin lesions, evaluated in clinical studies, provide subsidies for the development of new technologies and products, as well as scientific innovations that generate a distinct therapeutic alternative and provide less dependence on the use of anti-inflammatory drugs steroidal inflammatory drugs and antibiotics for healing skin lesions. It is also expected that this systematic review will provide data to health professionals in an attempt to inform, facilitate and increase adherence to new clinical protocols capable of promoting better health conditions for the patient.

4
  • ANA CRISTINA LA GUARDIA CUSTODIO PEREIRA
  • KNOWLEDGE OF DENTAL SURGEONS REGARDING HEMOSTATIC DISORDERS AND THE IMPACT ON DENTAL PRACTICE

  • Leader : MELINA DE BARROS PINHEIRO INACIO
  • MEMBRES DE LA BANQUE :
  • ANDRE DE OLIVEIRA BALDONI
  • MARIA CRISTINA DUARTE FERREIRA
  • MARIA DAS GRACAS CARVALHO
  • MELINA DE BARROS PINHEIRO INACIO
  • Data: 14 sept. 2023


  • Afficher le Résumé
  • Introduction: During dental care, the lack of knowledge on the part of the dental surgeon (DC) regarding the preoperative management of the patient with some hemorrhagic or thrombotic predisposition/condition (the latter using medication to reduce the hypercoagulable potential) can put into question the risk to the health of this patient. Dental practice with competence and safety requires knowledge that, until then, has been little explored by most dental surgeons (CDs). Familiarity with several laboratory tests that allow the evaluation of an individual's hemostatic response through the proper interpretation of their results is essential for the CD, since they have great clinical value. Objective: To describe and investigate factors associated with the knowledge of dentists in the State of Minas Gerais on hemostatic disorders, anticoagulant and antiplatelet drugs, and their laboratory approach in dental practice. Methods: This is a descriptive observational cross-sectional study involving a convenience sample of CDs in the State of Minas Gerais. Study participants answered a questionnaire that contained questions related to bleeding disorders, laboratory tests and their proper interpretation, the use of oral anticoagulants (warfarin and those of direct action - DOACs) and antiplatelet agents. The period for recruiting research participants was between August and December 2021. Additionally, the questionnaire was validated using the Delphi method. A quick reference manual is also being developed to help CDs be used in a safe and predictable manner. Results: For the present study, 389 CDs were eligible, of which the majority were female (65.3%). Of these, most reported not having knowledge about some coagulopathies and thrombocytopathy, and 77.9% and 57.1% only requested bleeding time (ST) and prothrombin time (PT), respectively, even when performing invasive procedures. Most (87.9%) have already assisted patients using drugs that interfere with hemostasis, such as oral anticoagulants and antiplatelet agents, but they have never faced a case of unexpected postoperative bleeding (60.4%). Most participants also reported never having observed oral petechial and gingival bleeding (70.5%). According to the established score, 313 (80.4%) participants were classified as having poor knowledge, 59 (15.2%) with fair knowledge, and 17 (4.4%) with good knowledge. Despite the CDs performing invasive procedures, their responses revealed a significant lack of knowledge about the use of anticoagulants and laboratory tests for their monitoring. In the validation of the questionnaire, the average of the professionals' scores showed values greater than 7.0 (70.0%), indicating a high degree of agreement between the panelists. Conclusion: The knowledge of the analyzed sample of CDs about hemostatic disorders and their implications in dental practice still seems to be insufficient. Although the study participants with specializations in implant dentistry and surgery and oral and maxillofacial traumatology achieved good performance, knowledge about the use of oral anticoagulants as well as their due monitoring still seems to be incipient. The questionnaire was validated, as it presented a level of agreement above 70.0% and could be applied in future research in the field of dentistry.

5
  • RICARDO AUGUSTO DOS SANTOS SILVA
  • Em breve.

  • Leader : MARIANA LINHARES PEREIRA
  • MEMBRES DE LA BANQUE :
  • MARIANA LINHARES PEREIRA
  • EDUARDO HENRIQUE DE MATOS LIMA
  • CARINA CARVALHO SILVESTRE
  • Data: 15 sept. 2023


  • Afficher le Résumé
  • Em breve.

6
  • EDNALVA DE SOUZA PEREIRA LIMA
  • Evaluation of the effects of medicines containing Cannabis sativa on the acitivity of the Cytochrome P450(3A4) in Wistar rats

  • Leader : WHOCELY VICTOR DE CASTRO
  • MEMBRES DE LA BANQUE :
  • VIRGINIA MARTINS CARVALHO
  • DANILO CESAR GALINDO BEDOR
  • WHOCELY VICTOR DE CASTRO
  • Data: 25 oct. 2023


  • Afficher le Résumé
  • Reports about the use of products derived from Cannabis sativa for religious, medicinal and recreational purposes date back more than two thousand years. Among the various phytochemicals present in this species, tetrahydrocannabinol (THC) and cannabidiol (CBD) should be highlighted. THC has antiemetic, antispasmodic, analgesic, and appetite-stimulating activities. CBD, on the other hand, has indications for the treatment of refractory epilepsy, anxiety, psychotic and pain disorders. Patients who need to use Cannabis-derived products have chronic illnesses and usually use the phytoderivative in association with several other conventional medications, increasing the risk of possible interactions. Studies involving the interaction of Cannabis-derived products with conventional medicines are scarce and contradictory. The objective of this work was to evaluate in vivo the effects of products containing Cannabis sativa commercially available in Brazil on the activities of the cytochrome P450 3A system (CYP 3A), the main metabolic pathway of drugs. For this, midazolam (MDZ) was used as a marker of the activity of this enzyme. The pharmacokinetic profile of  MDZ  (20 mg.kg-1) was determined  in the absence and presence of the commercial product CBD (20 mg.mL-1; 20 mg.kg-1) and the medicinal oil of THC and CBD (15 mg.mL-1; 20 mg.kg) in Wistar rats. Blood collections were performed at predetermined times. Plasma MDZ levels were quantified using a previously validated method using the HPLC technique, from which the following pharmacokinetic parameters were determined: plasma half-life (t1/2), total clearance (Cl/F), area under the curve of plasma concentration versus time (AUC), maximum plasma concentration (Cmax) and time to reach Cmax (tmax) and relative bioavailability (Fr). The Cannabis-derived products increased the AUC(0-6h) and Cmax  4- and 10-fold, respectively. The results suggest a probable enzymatic inhibition, which may compromise the pharmacological response of the drugs metabolized via  CYP3A in clinically relevant manner. Additional studies will be performed to confirm these results and to reduce interindividual variability.

7
  • FELLIPE ALEXANDRE ALVES MORAES
  • ASARALDEHYDE: EVALUATION OF THE ANTI-INFLAMMATORY, ANTI-NOCICEPTIVE ACTIVITY, ELUCIDATION OF THE PROBABLE MECHANISMS OF ACTION AND TOXICITY OF A BIOACTIVE METABOLITE FROM Duguetia furfuracea

  • Leader : ADRIANA CRISTINA SOARES DE SOUZA
  • MEMBRES DE LA BANQUE :
  • ADRIANA CRISTINA SOARES DE SOUZA
  • ELIAS BORGES DO NASCIMENTO JUNIOR
  • FARAH MARIA DRUMOND CHEQUER BALDONI
  • Data: 27 oct. 2023


  • Afficher le Résumé
  • About 30% of the world's adult population suffers from chronic pain. Among the main causes of chronic pain, the pathologies of inflammatory etiology stand out. Besides the drugs traditionally used not achieving the desired therapeutic outcome, they present a significant number of side effects that hinder the success of the treatment. Finding new pharmacological alternatives for the treatment of pain must be a priority for the scientific community. Ethnopharmacology has proven to be an important tool in the search for new pharmacological alternatives, by researching the traditional uses of natural products and their derivatives. This study had as its main objectives to evaluate the anti-inflammatory and antinociceptive activity, neuromotor effects, toxicity, as well as the probable mechanisms of anti-inflammatory and antinociceptive action through an in vivo model of asaraldehyde, a bioactive metabolite from Duguetia furfuracea (A. St.-Hil.) Benth. & Hook. f. To this end, we used the carrageenan-induced paw edema model to investigate the anti-inflammatory properties, the acetic acid-induced writhing, formalin, and hot plate tests to evaluate the antinociceptive properties, the rotating bar test (rotarod) to assess the neuromotor impact of asaraldehyde, and in silico ADMET (Absorption, Distribution, Metabolism, Excretion, and Toxicity) property evaluation software, along with the in vivo acute oral toxicity test, to determine the acute toxicity of asaraldehyde. The project was submitted to and approved by the UFSJ IACC. In the paw edema test, asaraldehyde showed antiedematogenic potential, reducing edema progression and inhibiting polymorphonuclear leukocyte migration. The induced abdominal contortions test signaled a reduction of the nociceptive response, the same could be observed in the formalin test in both phases. The hot plate test confirmed the central activity of asaraldehyde in all dosages. Any neuromotor changes from oral administration were ruled out in the rotarod test. When the effects of asaraldehyde associated with antagonists were evaluated in the formalin test, the participation of the opioid system in the mechanisms of antinociception was evidenced. The results confirm the information obtained in the literature about structural analogues of asaraldehyde (alpha-asarone, 2,4,5-trimethoxystyrene). In-silico toxicity evaluation, as well as acute oral toxicity tests, demonstrated that asaraldehyde has an adequate safety profile for prototype compounds with pharmacological potential, with an indication of hepatic changes only at the highest dose. Additional studies to evaluate pharmacokinetics, toxicity under prolonged exposure, as well as reproductive, mutagenicity, and carcinogenicity studies should be performed to confirm the findings described about its toxicity.

8
  • NATHANE STEFANIE DE QUEIROZ
  • ANALYSIS OF CLINICAL COMMUNICATION IN UNDERGRADUATE PHARMACY STUDENTS: A MULTICENTRIC STUDY

  • Leader : ANGELITA CRISTINE DE MELO
  • MEMBRES DE LA BANQUE :
  • ANTONIO EDUARDO MATOSO MENDES
  • MIGUEL MARQUES DA GAMA BARBOSA
  • AFONSO MIGUEL DAS NEVES CAVACO
  • ANGELITA CRISTINE DE MELO
  • Data: 31 oct. 2023


  • Afficher le Résumé
  • Introduction: Quantitative assessments of medication communication with patients have been performed and may contribute to improving education and training. For example, pharmacist-patient verbal communication provided by the Roter Interaction Analysis System (RIAS). Despite the similarity of communication in the provision of clinical services by pharmacists and the advances in teaching communication skills, there seems to be no data available that describe the clinical communication skills of pharmacy students at the end of the course.

    Methods: Analysis of the communication competence between pharmacist and patient using simulated patients with complaints of mild allergic rhinitis in comfort with 100 students of the last year of pharmacy from the five Brazilian regions. The video was recorded and was analyzed using the two main domains: biomedical (27 categories) and socio-emotional exchange (14 categories) of the Roter Interaction Analysis System. Statistical analyzes were performed using ANOVA, One Simple t Test, TAMNHANE and TUKEY multiple comparison tests. The codes that obtained a significant difference between the norms were highlighted, both for the task and socio-emotional codes.

    Results: Of the 100 interviews analyzed, an average duration of simulated consultations of approximately 14 minutes was observed. There was a significant difference according to the Brazilian region for number of speaker from the pharmacist/patient whose average was 183.4 (p= 0.048). In task codes and socio-emotional codes there was a significant difference between the codes when compared by region. Among them: personal for pharmacist (F=2.501, p=0.047); Approve for the pharmacist (F= 5.391, p < 0.001); R/O (F= 3.522, p< 0.010). And in biomedical communication counselling (F = 4.321, p = 0.003).

    Conclusion: The findings of the present study corroborate the existence of prescription in clinical communication in the public college of Brazilian pharmacy, more evident in higher education institutions in the North region of the country. Indicating that the disciplines, mainly, can be just observational, and have practice so that the skills of communication skills in health education have been acquired since of graduation. 

9
  • ALINE THAYNARA DE MOURA COELHO
  • MELATONIN AND SODIUM VALPROATE IN PREGNANCY: EFFECTS ON THE ANTIOXIDANT SYSTEM AND HEPATIC FUNCTION OF DESCENDANTS

  • Leader : LEANDRO AUGUSTO DE OLIVEIRA BARBOSA
  • MEMBRES DE LA BANQUE :
  • HELIO BATISTA DOS SANTOS
  • JÚLIO ALBERTO MIGNACO
  • LEANDRO AUGUSTO DE OLIVEIRA BARBOSA
  • Data: 23 nov. 2023


  • Afficher le Résumé
  • Melatonin is a hormone acting in organization of sleep as well as in fetal development. Low concentrations of this hormone in pregnancy seems to cause harms to the pregnant woman, the fetus and the offsprings. In contrast of it certain exogenous molecules such as sodium valproate may cause risks to organism development. Valproate is used in treatment of epilepsy and bipolar disorder tough it is known teratogenic effects when used in pregnancy. This study aimed to evaluate the maternal prenatal exposure to melatonin and sodium valproate to adolescent offsprings function and antioxidant system of the liver. First of all, levels of proteins, glucose, triglycerides, cholesterol and the Na+,K+-ATPase activity were determined in liver fractions, as an initial analysis of liver function. Then part of hepatocytes antioxidant system integrity was evaluated by measuring GSH, MDA and H2O2. Results showed no changes in Na+,K+-ATPase activity and macronutrient content in groups whose mothers were exposed to valproate or melatonin compared to the control. Likewise no significant changes were observed in the content of GSH, MDA and H2O2. These results suggest that the liver function and antioxidant system in adolescent rats are not influenced by maternal prenatal exposure to sodium valproate or melatonin. These results can be due to a specificity of the liver tissue since studies under conditions similar to those applied here showed significant changes in the analyzed markers in other organs. Other evaluations will be carried out but in general lines this study strengthens the evidence about a non heavily explored topic in the scientific community so far which is the role of maternal exposure to melatonin and valproate in development of the offsprings liver – an essential organ for organism functions.

10
  • MARIELLE FREITAS ARAUJO DIAS
  • Treating Children with ASD: The Perspective of Caregivers.

  • Leader : MARCELO GONZAGA DE FREITAS ARAUJO
  • MEMBRES DE LA BANQUE :
  • JÉSSICA BRUNA SANTANA SILVA
  • ELAINE CRISTINA RODRIGUES GESTEIRA
  • MARCELO GONZAGA DE FREITAS ARAUJO
  • Data: 1 déc. 2023


  • Afficher le Résumé
  • Autism spectrum disorder (ASD) is a neuropsychiatric disorder that develops in childhood and involves deficits in social interaction, communication and repetitive behaviors. The prevalence of ASD represents a significant public health problem, demanding attention due to its emotional and economic impact on families and society. The challenges of parents and/or caregivers of children with ASD are well recognized, but their perspective on their child's treatment has been overlooked. This study aimed to investigate the perspective of parents/caregivers on the effectiveness of treatments and services offered to their children. A data collection instrument was validated for this research according to cross-cultural validation methodologies of conceptual and item equivalence. The validated questionnaire was applied to 30 parents/caregivers of children with ASD for data collection. The results were analyzed using Bardin's content analysis, which were categorized into symptoms, well-being, treatments and general support. However, the common manifestations of the parents were mainly related to the beneficial effects of the therapies on the well-being of the children, in the remarkable reduction of aggressiveness and improvement in the quality of sleep with the use of risperidone, and positive responses in the correction of the food selectivity of the children. children. The school emerged as an important support factor, as well as the support from friends, which are often more notable than the support from the family itself. The lack of specialized services in the SUS and the high cost of therapies were also critical points reported by parents. Ultimately, the study gave parents and caregivers a voice to provide real-world evidence on how to improve their children's health and well-being and help children with ASD and their families.

2022
Thèses
1
  • GUSTAVO FERNANDO DE FRAZÃO LIMA
  • -

  • Leader : JOSE AUGUSTO FERREIRA PEREZ VILLAR
  • MEMBRES DE LA BANQUE :
  • JOSE AUGUSTO FERREIRA PEREZ VILLAR
  • JEFFERSON LUIZ PRINCIVAL
  • RAFAEL AUGUSTO SOLDI
  • Data: 4 févr. 2022


  • Afficher le Résumé
  • -

2
  • DENNER HENRIQUE ISAIAS SOUZA
  • Analysis of the quality of evidenceon the effectiveness of direct oral anticoagulants

  • Leader : DANYELLE ROMANA ALVES RIOS
  • MEMBRES DE LA BANQUE :
  • DANYELLE ROMANA ALVES RIOS
  • LEILISMARA SOUSA NOGUEIRA
  • MARIA AUXILIADORA PARREIRAS MARTINS
  • NAYARA RAGI BALDONI COUTO
  • Data: 7 févr. 2022


  • Afficher le Résumé
  • Introduction: Direct oral anticoagulants (DOACs) have been presented as a new
    anticoagulant front in the prevention of thromboembolism. Imprecisions in design,
    conduct, and analysis may bias the effect of the intervention to be underestimated
    or overestimated. Objective: To analyze the quality of scientific evidence on the
    efficacy of DOACs available in package leaflets. Method: To achieve the
    objective, the study was divided into two stages: (i) identification and analysis of
    the availability of bibliographic references contained in the package leaflets of
    DOACs consulted through the electronic bularium of the Agência Nacional de
    Vigilância Sanitária (ANVISA); and (ii) analysis of the quality of the studies
    contained in the package leaflets using. In this step the Cochrane Risk of Bias
    tool was used. This tool has seven domains, and each domain was classified as
    high, uncertain, or low risk of bias according to the authors' assessment. The
    analysis was performed by two independent authors. In case of disagreement, a
    third reviewer was consulted. After evaluation, graphs of the analyses were
    generated using Revman 5.4 software. Results: Twenty-five studies were
    evaluated. The results showed that the clinical trials used as references in the
    professional package leaflets of DOACs have great methodological uncertainties.
    Conclusion: Therefore, the present study evidenced that the quality of clinical
    trials used as references for DOACs' package inserts showed many uncertain
    domains in their clinical trials and should be analyzed with caution. It is suggested
    the need for strict protocols that establish the description of the methods in a clear
    and cohesive way.

3
  • MARLON SILVA TINOCO
  • DEPRESCRIPTION OF BENZODIAZEPINES IN THE ELDERLY: ANALYSIS OF PACKAGE INSERTS AND VALIDATION OF INSTRUMENTS FOR IDENTIFICATION OF SIGNS AND SYMPTOMS OF WITHDRAWAL

  • Leader : ANDRE DE OLIVEIRA BALDONI
  • MEMBRES DE LA BANQUE :
  • ANDRE DE OLIVEIRA BALDONI
  • CAMILO MOLINO GUIDONI
  • LORENA ROCHA AYRES
  • Data: 10 févr. 2022


  • Afficher le Résumé
  • With population aging and the irrational use of benzodiazepines by society, health professionals need quality evidence to perform the deprescription of these drugs, as well as instruments for this process to occur effectively and safely for the elderly. That said, this study has been divided into two chapters.

    Chapter 1: Objective: To analyze the presence and quality of content on drug deprescribing in Brazilian package inserts for benzodiazepine drugs Methodology: A documentary study was carried out, in which data contained in Brazilian package inserts for benzodiazepines (BZDs) were analyzed. The keywords “reduction”, “withdrawal” and “non-prescription” were sought. Furthermore, information relevant to the topic (directly related to the describing process) was verified, such as the maximum time of use, the risks of prolonged use and adverse reactions. For purposes of comparison of information, explicit and systematic guidelines for describing and warning of adverse reactions found in the literature were considered. Results: Twelve BZD inserts were found with valid registration and the inserts (100%) address the topic of descrip- tion through the suggestion of gradual dose reduction. Only one (8.33%) presented explicit and systematic guidance for dose reduction. As for the maximum time of use, different information according to each drug, and a package insert (8.33%) did not present this information. Regarding the risks of prolonged use of the drug, 11 (91.67%) package inserts reported a risk of dependence and/or tolerance and one (8.33%) stated that the data available in the literature do not provide a true estimate of the risk. 33.33% package inserts did not address the risk of falls and five (41.67%) did not address the risk of fractures in the elderly. The risk of cognitive impairment was not reported in six (50.00%) package inserts. The risk of a car accident is included in the 12 (100%) package inserts analyzed. Conclusion: The package inserts do not provide detailed or schematic descriptions. It is extremely important that health professionals are guided in their conduct and, therefore, there is a great need to update the package inserts.

     

    Chapter 2: Objective: to develop and carry out the validation of an adequate instrument to assess the signs and symptoms of withdrawal from the benzodiazepine deprescription process in Brazilian elderly. Methodology: a two-step methodological study was carried out, the first being the preparation of an instrument to identify signs and symptoms arising from the gradual descrip- tion of benzodiazepines in elderly Brazilians. A second stage consisted of the validation of the instrument using the delphi technique by experts in the area of health and specialists in elderly health. Results: A systematized elaboration and validation was carried out with 36 experienced reviewers in elderly care, making it possible to build an instrument for the proper identification of withdrawal signs and symptoms present during the process of describing benzodiazepines in elderly Brazilians. The agreement percentage found was higher than the minimum awarded rate of 80% and the critical evaluation of the evaluators updated the improvement of the questionnaire. Conclusion: The instrument elaborated understood the signs and common to the process of describing BZD in the elderly, validated by a reviewer to be applicable in the Brazilian health context.

4
  • ADRIANO LOPES DA SILVA
  • CHEMICAL COMPOSITION AND EVALUATION OF THE ANTIOXIDANT AND LARVICIDE ACTVITIES OF THE EXTRACTS, FATTY ACIDS AND METHYL ESTERS OF THE PERICARP OF Tecoma stans (L.) Juss. Ex Kunth (BIGNONIACEAE).

  • Leader : LUCIANA ALVES RODRIGUES DOS SANTOS LIMA
  • MEMBRES DE LA BANQUE :
  • LUCIANA ALVES RODRIGUES DOS SANTOS LIMA
  • MAIRON CESAR COIMBRA
  • NATHÁLIA LUCCA SILVA
  • Data: 29 avr. 2022


  • Afficher le Résumé
  • Currently, there is a growing interest in antioxidants and insecticides from plant extracts from aromatic and medicinal plants, cereals, fruits, considered sources of natural antioxidants and bioinsecticides, and this is also economically interesting. The control of insects with synthetic insecticides causes a great impact on the environment and, consequently, on public health, as they are not biodegradable and because they are bioaccumulative. In this context, the ipê-mirim, guarã-guarã, ipê-de-jardim, amarelinho and yellow caroba, some of the names by which the species Tecoma stans (L.) Juss. Ex Kunth (Bignoniaceae) is known, it becomes interesting. Thus, this study aimed to evaluate the antioxidant activities and larvicidal potential of the T. stans pericarp from hexane extracts (HE), methanol extract (ME) and their fatty acids (FA) and methyl esters (FAMES). The extracts were obtained with hexane and methanol in a Soxhlet apparatus, hydrolyzed and esterified to obtain AG and FAME, respectively, and analyzed by Gas Chromatography and Mass Spectrometry (GC-MS) to determine their composition. In the phytochemical screening, the presence of steroids, triterpenes, alkaloids and coumarins was detected for HE, whereas for ME only the presence of alkaloids and coumarins was verified. In the analysis by GC-MS, the nonacosane hydrocarbon was identified as the major compound (81.69%) in the hexane extract. In FAME (EH), the major compounds were methyl hexadecanoate or palmitate (C16:0), methyl 9, 12-octadecadienoate or linoleate (C18:2) and methyl 9, 12, 15-octadecatrienoate or linolenate (C18:3), whereas in FAME (ME), the major compound was methyl hexacosanoate (C26:0). In the evaluation of antioxidant activity, the samples were effective at all concentrations tested (1, 10, 100, 250 and 500 µg/mL) by the DPPH method, with antioxidant potential, with percentages of inhibition ranging from 63.70 to 96.10% at a concentration of 500 µg/mL. The samples were more effective than BHT in capturing DPPH radicals, because they had lower EC50 values. In the FRAP test, the samples showed a lower antioxidant potential. By this methodology, the highlight was for the methanolic extract that had an activity like that of BHT. The results for lethality in A. salina showed that the FAME of the hexane extract and the FAME of the methanolic extract can be considered toxic with LD50 < 1000 µg/mL. In the susceptibility test in Culex quinquefasciatus larvae, it was possible to observe that the hexane extract and its fatty acids have larvicidal activity, because they promoted larval mortality. However, this effect is considered ineffective according to the literature. Thus, it can be concluded that this work is unprecedented and important because it contributed to the identification of compounds present in the pericarp of T. stans, as well as to the evaluation of the biological activities of this species.

5
  • AMANDA MARIA DE PAIVA
  • Use of medicines in pediatrics: an analysis of the technical and legal aspects of package inserts

  • Leader : ANDRE DE OLIVEIRA BALDONI
  • MEMBRES DE LA BANQUE :
  • ADRIANO MAX MOREIRA REIS
  • ANDRE DE OLIVEIRA BALDONI
  • MARCIA CHRISTINA CAETANO ROMANO
  • Data: 29 avr. 2022


  • Afficher le Résumé
  • Background: The use of medication in pediatrics should be performed with caution. The
    shortage of studies in the area and of specific medicines formulated for children and adolescents
    that address the physiological needs of this population make the occurrence of adverse drugrelated events more common in this population when they’re compared to adults. The
    medication package inserts are legal documents that provide information about the use of
    medicines to patients and health professionals and must contain data that guide the
    administration and inform the potential risks associated with medicines. Based on the above,
    this study aims to perform the technical and legal analysis of package inserts for medicines used
    in pediatrics, verifying whether the package inserts include technical and legal aspects and
    corroborate the recommendations of clinical protocols and legal provisions.
    Methods: A
    documental study of medication package inserts analysis was carried out, divided into two
    substudies. The search of the medication package inserts was carried out through the electronic
    portal of the National Health Surveillance Agency (Agência Nacional de Vigilância Sanitária
    – ANVISA). Substudy 1 consists of the technical and legal analysis of drug inserts commonly
    used via continuous infusion (CI) pump in neopediatric intensive care units (ICU). Thirteen
    drugs used via IC were selected and information on the use in neonatal and pediatric patients
    was searched in the package inserts. In order to compare the technical information, the
    Micromedex Neofax®, Micromedex Pediatrics® and Lexicomp® databases were consulted.
    To analyze the legal aspects, ANVISA's Resolution of the Collegiate Board (
    Resolução da
    diretoria colegiada -
    RDC)47/2009 was used. Substudy 2 consists of the search for
    recommendations on adverse drug reactions (ADR) in pediatrics contained in the Guideline
    Key Potentially Inappropriate Drugs in Pediatrics: The KIDs List in the package inserts of drugs
    sold in Brazil.
    Results: substudy 1 showed that of the 13 drugs analyzed, 46% (n=6) showed
    some non-compliance with RDC 47/2009. Only 46% (n=6) of the drugs are indicated for
    pediatric use and only the midazolam package insert (7.7%) contained information considered
    essential for use via CI in pediatrics. Substudy 2 showed that, of the drugs indicated for pediatric
    use, only 26% (n=6) presented the risks of ADRs similar to the Guideline KIDs List and the
    same recommendations on the package inserts. The others (74%) showed some disagreement.
    Conclusions: The package inserts are technical and legal documents that provide essential
    information for the safe use of medications and, as noted, they have deficiencies that can
    compromise the safety of medication use, especially when dealing with neonatal and pediatric
    patients.


6
  • MARA LUIZA DE PAIVA DOMINGUES
  • Assessment of the risk of bias of clinical trials referenced in the Technical Notes for Ordering Direct Oral Anticoagulants

  • Leader : ANDRE DE OLIVEIRA BALDONI
  • MEMBRES DE LA BANQUE :
  • ANDRE DE OLIVEIRA BALDONI
  • CARINA CARVALHO SILVESTRE
  • KÉRILIN STANCINE SANTOS ROCHA
  • LEILISMARA SOUSA NOGUEIRA
  • Data: 4 mai 2022


  • Afficher le Résumé
  • Introduction: Started in the 1990s, the judicialization of health care in Brazil contemplates direct oral anticoagulants (DOAC). The increase in demand resulted in the creation of the “eNatJus” system, which stores Technical Notes (NTs) that assist in the decisions of health processes. Thus, studies to evaluate the quality of clinical trials used in the elaboration of NTs are necessary and allow greater security in decisions. Objective: To evaluate the clinical trials that make up the NTs referring to the DOAC by classifying the risk of bias. Methods: The NTs related to apixaban, dabigatran, edoxaban and rivaroxaban DOAC requests were selected on the e-NatJus website and, after excluding duplicate bibliographic references, an analysis of the clinical trials used for their writing was performed. The risk of bias of clinical trials (low, high or uncertain bias) was assessed using the Cochrane Risk of Bias tool and the results of the analyzes were added in Review Manager 5.4 (Revman 5.4) for graphing. Results: 181 NTs were selected, 236 articles were analyzed and after applying the inclusion criteria, 28 clinical trials were analyzed in full. None of the clinical trials were free from bias. The majority of clinical trials, 71% (20/28) showed low risk of bias with respect to attrition bias and reporting bias. On the other hand, 61% (17/28) of the trials did not control for selection, performance and detection bias, since they present uncertainties and high risk of bias. In addition, it was observed that 21% (6/28) of clinical trials had risk of bias for conflict of interest. Conclusion: The biases present in the clinical trials cited as a bibliographic reference of the NT referring to the DOAC request are significant and compromise their quality.

7
  • Jéssica Spínola Silva
  • EVALUATION OF THE PERFORMANCE OF A SEROLOGICAL METHOD BY FLOW CYTOMETRY FOR THE GENOTYPE-SPECIFIC DIAGNOSIS OF CHAGAS DISEASE

  • Leader : MELINA DE BARROS PINHEIRO INACIO
  • MEMBRES DE LA BANQUE :
  • MELINA DE BARROS PINHEIRO INACIO
  • GUSTAVO MACHADO ROCHA
  • GLAUCIA DINIZ ALESSIO
  • LEILISMARA SOUSA NOGUEIRA
  • Data: 12 mai 2022


  • Afficher le Résumé
  • Trypanosoma cruzi was subdivided into six distinct genetic groups, called Discrete Typing Units (DTUs), from TcI to TcVI. The genetic variability of T. cruzi can influence the biological characteristics of the parasite, the epidemiology and clinical features of Chagas disease (DCh). Furthermore, individuals infected with different T. cruzi genotypes may present a distinct therapeutic response to the treatment of DCh. Therefore, the development of new methods for the genotype-specific diagnosis of DCh is of great importance for clinical, epidemiological and post-therapeutic monitoring studies. Some molecular methods used for this proposal have limitations, such as: the need for previous isolation of the parasite from the blood by low-sensitivity techniques (blood culture and xenodiagnosis) that can select T. cruzi DTUs, in addition to the need to use multiple targets of the parasite's genome for the correct identification of the genetic groups that infect the host. The serological methods developed for the genotype-specific diagnosis of DCh were not able to recognize all T. cruzi DTUs present in samples of individuals from different geographic regions. In this sense, the technique of investigation of anti-amastigote (A), trypomastigote (T) and epimastigote (E) antibodies of T. cruzi by flow cytometry (Chagas-Flow ATE) for the diagnosis of DCh was standardized. Initially, Chagas-Flow ATE was developed for the genotype-specific diagnosis of experimental infection by T. cruzi, showing a good performance to discriminate the different DTUs of the parasite that infect mice. Therefore, continuing these studies, the objective of this project was to evaluate the performance of Chagas-Flow ATE in the universal and genotype-specific diagnosis of DCh, using human serum samples. 102 sera from patients with Chagas disease (CH) in the chronic phase, infected with TcI, TcVI or TcII of T. cruzi DTUs, using the Chagas-Flow ATE, were evaluated. The results showed that this new methodology showed excellent accuracy (100%) in the universal diagnosis of DCh, being able to segregate all serum samples from CH patients from those not infected with T. cruzi (NI), using certain sets of attributes (target antigens, serum dilutions and cut-off points). Furthermore, analysis by decision trees, using selected sets of attributes, indicated an excellent performance of Chagas-Flow ATE in the genotype-specific diagnosis of DCh, with an accuracy of 92% and 97% to discriminate infections by “TcI vs TcVI vs TcII” and “TcI vs TcII”, respectively. The data obtained suggest the use of Chagas-Flow ATE in the universal and genotype-specific diagnosis of DCh. Thus, through this new method, it will be possible to interfere with the T.cruzi genotypes that infect the patient, having a practical impact on the epidemiological surveillance of this disease, in the clinic and in the post-therapeutic monitoring of individuals with Chagas disease.

8
  • Magda Cesar Raposo
  • Drug-Food Interactions in Brazilian Drug Data Sheets: Is There Concordance With The Literature?

  • Leader : DANYELLE ROMANA ALVES RIOS
  • MEMBRES DE LA BANQUE :
  • DANYELLE ROMANA ALVES RIOS
  • RAFAEL SANTOS SANTANA
  • RENATA ADRIANA LABANCA
  • Data: 19 juil. 2022


  • Afficher le Résumé
  • Objective: To analyze the existence of information about drug-food interactions in
    Brazilian drug package inserts and whether there is agreement with the literature.
    Methodology: Electronic package inserts for patients and health professionals, made
    available by Anvisa, were analyzed. We identified 144 drugs with food interactions, of
    these 15 were excluded, because they were registered as expired/canceled or non-
    existent in Anvisa&#39;s files. After identifying all package inserts, a comparative analysis
    was performed between the information contained in the Interactions Manual, the health
    care professional&#39;s package insert, and the patient&#39;s package insert, and then we
    categorized these results. For data analysis and interpretation, a database was
    constructed in spreadsheet form using Microsoft Office Excel 2016. Results: Of the 129
    package leaflets analyzed, in 66 (51.2%), the information contained in the professional
    and patient leaflets agreed with each other, in 45 (34.9%) there was no information
    about interactions between drugs with food in both the professional and patient leaflets,
    in 13 (10.0%) the data contained in the professional and patient leaflets partially agreed,
    and in 05 (3.9%) there was information that did not agree between the two information
    sheets. In addition, there is no information on interactions between drugs and food in
    the professional package leaflet. In the comparison between the patient package insert
    and the Interactions Manual, in 45 (34.9%) there was no data on drug-food interactions
    in the patient package insert. In 36 (27.9%) the information agreed with each other, in
    30 (23.3%) the information in the patient package leaflet partially agreed with the
    Interactions Manual, and in 18 (13.9%) there was no agreement between the two
    sources of information. Partial Considerations: We found considerable divergence and
    absence of information, both in professional and patient package leaflets, about drug-
    food interactions. The absence or poor quality of information about drug-food
    interactions in package leaflets reduces their value as informational/educational material
    for the health care professional and patient. These factors may lead to risk exposure
    related to changes in drug response and patient safety.


     
9
  • LIVIAN RABELO LOPES
  • Drug repositioning: evaluation of the anti-inflammatory and antinociceptive activity of nitazoxanide

  • Leader : ADRIANA CRISTINA SOARES DE SOUZA
  • MEMBRES DE LA BANQUE :
  • ADRIANA CRISTINA SOARES DE SOUZA
  • ALINE APARECIDA SALDANHA RAFANTE
  • GLAUCIA MARIA LOPES REIS
  • MELISSA GRAZIELLE MORAIS
  • PRISCILA TOTARELLI MONTEFORTE
  • Data: 5 sept. 2022


  • Afficher le Résumé
  • Nitazoxanide (NTZ) is a drug widely used for the treatment of a wide variety of parasitic diseases. Some recent discoveries suggest new therapeutic functions of this drug, a process called drug repositioning. New drug development is a slow and costly process, so drug repositioning is an effective alternative approach. Inflammatory processes are part of the pathogenesis of several very prevalent diseases. Inflammation is a complex biological process and its chronicity can lead to deleterious effects in the body. The treatment of inflammation involves the use of non-steroidal anti-inflammatory drugs, glucocorticoids and opioids, but the prolonged use of these drugs can cause serious adverse effects. Therefore, the search for new safer drugs is necessary. The objectives of this work were to evaluate the anti-inflammatory and antinociceptive activities of NTZ; using experimental models of paw edema induced by carrageenan (Cg), model of abdominal writhing induced by acetic acid, formalin test and rota rod for analysis of motor impairment and balance. The oral (po) treatment of the animals with NTZ  induced a significant inhibition of paw edema of 60% and 68% at doses of 10 and 30 mg/Kg, respectively, already in the first hour after Cg injection. There was also a significant inhibition 3 and 4 hours after the inflammatory stimulus at doses of 10 mg/Kg (57.14% and 21.27%) and 30 mg/Kg (69.25% and 61.70%) and 6 hours after stimulation at a dose of 30 mg/kg (40.47%). Histological analysis of the footpads of animals treated with NTZ 30 mg/Kg (4 hours after Cg stimulation) showed a reduction in the migration of inflammatory cells by 65.77%. Likewise, treatment with NTZ 30 mg/Kg reduced tumor necrosis factor-α (TNF-α) levels in the footpad 2 hours after Cg-induced inflammation. NTZ showed antinociceptive activity, significantly inhibiting the number of abdominal contortions induced by acetic acid at doses of 30 and 90 mg/kg by 54.14% and 56.21%, respectively. In the formalin test, doses of 10 and 30 mg/Kg did not present an antinociceptive effect; and the dose of 90 mg/Kg had an effect in both phases of the test (55.88% and 57.99%). Finally, the treatment of animals with NTZ, at doses of 10, 30 and 90 mg/Kg, did not cause any motor or balance alteration in the rota rod test. This result suggests that the antinociceptive effect of NTZ may be related to the reduction of inflammatory pain by inhibiting inflammation. The results suggest an anti-inflammatory effect, possibly related to the decrease in TNF-α.

10
  • PAULA LAMOUNIER LIMA
  • EPIDEMIOLOGICAL PROFILE OF THE SEIZURE OF MARIHUANA, COCAINE AND CRACK IN MUNICIPALITIES IN THE CENTRAL-WEST REGION OF MINAS GERAIS

  • Leader : JOAQUIM MAURICIO DUARTE ALMEIDA
  • MEMBRES DE LA BANQUE :
  • DANYELLE ROMANA ALVES RIOS
  • FARAH MARIA DRUMOND CHEQUER BALDONI
  • JOAQUIM MAURICIO DUARTE ALMEIDA
  • YURI MACHADO
  • Data: 28 sept. 2022


  • Afficher le Résumé
  • Drug use and abuse is a serious public health problem, having a strong relationship with conditions of social vulnerability and crimes in general, such as trafficking and violence. The objective of the present study was to describe the profile of arrests of marijuana, cocaine and crack, carried out by the Civil Police, in the Central-West region of Minas Gerais. This is a descriptive observational study designed according to the guidelines proposed by the Declaration of Strengthening Reports of Observational Studies in Epidemiology (STROBE), carried out on a documentary basis from January 2017 to December 2019. Data collection took place on the 1st Regional Civil Police Department of the 7th Civil Police Department, based in Divinópolis, with cases selected from the municipalities of Divinópolis, Itaúna, Cláudio, Carmo do Cajuru and Itatiaiuçu. The number of arrests per municipality, the nature of the crimes related to these arrests, the number of perpetrators involved in the arrests, the sociodemographic profile of the individuals arrested, the type and forms of the drugs arrested were determined. The database was created using the Questionnaire Development System (QDS) version 2.6.1.1 program, and later, the data were exported to the Statistical Package for the Social Sciences (SPSS) version 21 for statistical analysis. A total of 5,903 documents related to drug arrests were analyzed during the study period. The city that anchors the largest number of drug arrests is the municipality of Divinópolis (64%). Regarding the nature of the fact, the crime most correlated with these apprehensions belongs to illicit drug trafficking (59.2%), and which mostly involved more than one perpetrator (57.8%). The profile of individuals arrested for involvement with drugs was mostly male (90.8%), with a median age of 22 years, literate (32.8%) and single (67%). Among the drugs arrested, marijuana ranked first (63.9%), followed by cocaine (18.5%) and crack (17.7%). The form that most appears in the apprehensions is the loofah for marijuana (31%), paper bags for cocaine (82.3%) and rocks for crack (81.6%). Knowledge about the profile of drug arrests in the Central-West region of Minas Gerais becomes an important reference to assist in the planning of public policies for prevention and awareness according to the real demand, against the trade and consumption of drugs.

11
  • Patricia Costa Souza de Sá
  • EVALUATION OF HEMATOLOGICAL PARAMETERS IN PREGNANT WOMEN WITH AND WITHOUT PERIODONTAL DISEASE

  • Leader : MELINA DE BARROS PINHEIRO INACIO
  • MEMBRES DE LA BANQUE :
  • MELINA DE BARROS PINHEIRO INACIO
  • DANYELLE ROMANA ALVES RIOS
  • MARIA DAS GRACAS CARVALHO
  • MAURO HENRIQUE NOGUEIRA GUIMARAES DE ABREU
  • Data: 30 sept. 2022


  • Afficher le Résumé
  • Prenatal care is of fundamental importance and, therefore, must be carried out by a multidisciplinary health team, since numerous physiological, physical, psychological and even pathological changes can be perceived in this period. In addition to medical follow-up, imaging and laboratory tests, it is important to have specific dental care, as several changes and complications can occur during pregnancy with repercussions on the oral cavity. Therefore, the present study aims to analyze the hematological profile of pregnant women with and without periodontal disease (PD). This is a cross-sectional study developed in the city of Divinópolis/MG, Brasil. Data on pregnant women in the second and third trimesters were obtained by consulting medical and dental records, which were stratified into two subgroups: pregnant women with PD (n=107) and pregnant women without PD (n=42). Study variables were related to PD, sociodemographic, clinical and laboratory characteristics. Data were collected from the complete blood count of all participants and the indices derived from blood count parameters were calculated: NLR (neutrophils/lymphocytes ratio); PLR (platelet/lymphocyte ratio); LMR (lymphocyte/monocyte ratio); dNLR (derived NLR); SIRI (systemic inflammation response index); AISI (aggregate index of systemic inflammation) and SII (systemic immune-inflammation index). Data were analyzed using conventional statistical analysis using the Statistical Package for the Social Sciences® software (version 19.0), with a significance level of p<0.05. Regarding the results, the mean age in both subgroups was 27 years. As expected, pregnant women with less education had more PD. As for the hematological changes in the red blood cells,  the mean corpuscular volume of erythrocytes (MCV) was significantly higher in pregnant women with PD, probably a reflection of folate deficiency. Regarding the alterations in the white  blood cells, the global number of leukocytes and lymphocytes was significantly higher in pregnant women with periodontitis, possibly reflecting an inflammatory process caused by bacterial invasion of the periodontium, with systemic repercussions. Our study reinforces the need for a multidisciplinary team, including a dentist, in prenatal care, given that pregnancy is known to be a period subject to many physical, psychological and even pathological changes, bringing security to pregnant women in relation to their health. and the baby.

12
  • Paula Resende Daher Chaves
  • PHARMACEUTICAL CARE IN THE MANAGEMENT OF HEARTBURN AND DYSPEPSIA IN A COMMUNITY PHARMACY

  • Leader : MARIANA LINHARES PEREIRA
  • MEMBRES DE LA BANQUE :
  • MARIANA LINHARES PEREIRA
  • ADRIANA CRISTINA SOARES DE SOUZA
  • THAYS SANTOS MENDONCA
  • Data: 25 oct. 2022


  • Afficher le Résumé
  • Sem resumo (qualificação)

13
  • Natália Ribeiro Nunes
  • EVALUATION OF THE INFLAMMATORY PROFILE OF PATIENTS VICTIMS OF OPHIDIC ACCIDENTS SEEN AT THE JOÃO XXIII ER HOSPITAL, BELO HORIZONTE/MG

  • Leader : MELINA DE BARROS PINHEIRO INACIO
  • MEMBRES DE LA BANQUE :
  • MELINA DE BARROS PINHEIRO INACIO
  • FARAH MARIA DRUMOND CHEQUER BALDONI
  • LUCI MARIA SANT ANA DUSSE
  • RITA CAROLINA FIGUEIREDO DUARTE
  • Data: 28 oct. 2022


  • Afficher le Résumé
  • Among the species present in the Brazilian fauna, snakes of the Bothrops and Crotalus genera are responsible for the highest frequency of snakebites in our country. Such accidents, although still neglected, constitute a serious public health problem. Snake venom is a complex mixture of toxins, enzymes, activators and growth factor inhibitors that have deleterious effects on cells. Among such harmful effects, hematological abnormalities emerge as the most common of snake envenomation, with consumption coagulopathy being the most frequent and important. Given the above and due to the frequency and potential severity of snakebites, in addition to the difficulties encountered in their management, the present study aimed to survey the literature on the main snakebites (Bothrops and Crotalus) in our country, in addition to know the laboratory profile of victims of such accidents treated at Hospital de Pronto Socorro XXIII (HPS XXIII), in Belo Horizonte - MG, before and after antivenom use. Clinical and routine laboratory data were obtained from the patients' medical records. From the blood count data, the following ratios/indices were calculated, namely: NLR, PLR, RML, dNLR, SIRI, AISI and SII. Data analysis revealed that the vast majority of victims were male for both genders of snakes, with Crotalus accidents being the most serious. As for routine laboratory parameters, in bothropic accidents, the PT/INR showed an increased value before the antivenom serum, reflecting hemostatic disorders. Likewise, PT/RNI was also shown to be increased in crotalic accidents before antivenom serum, possibly reflecting low fibrinogen levels. As for the number of platelets, a drop in their number was observed after the antivenom serum. Before the serum, a reduction in MCH was also observed, which increased after the antivenom use. Ratios/indices derived from complete blood count parameters showed inconsistent results, not adding value to routine data. The data analyzed together allowed us to conclude that snakebites, Bothrops or Crotalus, are associated with several laboratory disorders, particularly hemostatic, of varying severity, but potentially fatal; Crotalus accidents, as expected, were more severe compared to bothrops; and the use of antivenom sérumtends to normalize the results of laboratory tests, indicating an evident benefit to patients. In view of the above, rapid admission to a specialized service for appropriate treatment on an urgent basis is mandatory.

14
  • Aline Michelle Silveira Silva
  • Relationship between drug trafficking and/or consumption and homicides in a region in the Midwest of Minas Gerais

  • Leader : FARAH MARIA DRUMOND CHEQUER BALDONI
  • MEMBRES DE LA BANQUE :
  • CRISTINA SANCHES
  • DANYELLE ROMANA ALVES RIOS
  • FARAH MARIA DRUMOND CHEQUER BALDONI
  • JOAQUIM MAURICIO DUARTE ALMEIDA
  • LEILISMARA SOUSA NOGUEIRA
  • Data: 30 nov. 2022


  • Afficher le Résumé
  • Introduction: Homicide is a heinous crime that has increased throughout the national context, making it a concern mainly for public entities. In 2016 homicides in Brazil represented 13% of the world total for the year. Drug use and markets can act as facilitators for violence. It was assumed that crimes related to consumption and/or drug trafficking increase the risk of the arrested individual committing a homicide or being a victim of this crime. Objectives: To assess the risk of individuals apprehended for drug consumption and/or trafficking committing homicide or being a victim of this crime, analyze the profile of the individual apprehended for trafficking and/or consumption of marijuana, cocaine/crack, describe the seized illicit drug and identify the rates of illegal drug trade in the cities of Divinópolis, Itaúna, Itatiaiuçu, Carmo do Cajuru and Cláudio. Methodology: This is an observational retrospective cohort study based on documents from 01/01/2017 to 12/31/2019. Data were collected at the Regional Civil Police Station and at the Regional Technical Session of Criminalistics in Divinópolis. Data processing and storage were carried out in a database, created using QDS™ Version 2.6.1 software and Microsoft Office Excel. To characterize the epidemiological profile, a descriptive statistical analysis of the variables was performed. This project was approved by the Research Ethics Committee involving human beings (CAAE: 30655120.3.0000.5545). Results: A total of 3,994 documents were selected from among those analysed, these being preliminary and definitive toxicological expert reports of seized illicit drugs and Social Defense Event Records - REDS. Divinópolis had the highest rate of registrations (63.32%), and marijuana was the most common drug (64.52%). 5944 individuals were identified in drug trafficking and/or consumption, 90.723% male, more frequent in the 18-40 age range in both genders (72.17% men and 71.87% women). Of the 194 homicides registered in the region during the studied period, 146 were involved with drugs, thus presenting a Relative Risk of 10.10 (p<0.0001). Conclusion: Divinópolis has the highest rate of drug seizures in the region, with marijuana being the most learned drug, following national and global standards. Young males prevail both in drug trafficking and/or consumption and in homicides. It can be seen that in this region studied, an individual inserted in the drug market and consumption increases the risk of committing or being the victim of a homicide.

15
  • SABRINA PAULA PEREIRA
  • Infection caused by carbapenem-resistant gram-negative bacteria in patients with chronic kidney disease: a systematic review

  • Leader : CAROLINE PEREIRA DOMINGUETI
  • MEMBRES DE LA BANQUE :
  • CAROLINE PEREIRA DOMINGUETI
  • CLÁUDIA NATÁLIA FERREIRA
  • MAGNA CRISTINA DE PAIVA
  • WILLIAM GUSTAVO DE LIMA
  • Data: 2 déc. 2022


  • Afficher le Résumé
  • Introduction: Chronic kidney disease is characterized by the progressive and irreversible loss of kidney function. About 144,7779 patients are on dialysis in Brazil. Infections caused by carbapenem-resistant Gram-negative bacteria are associated with higher mortality, longer hospital stay and greater economic burden. The increased resistance of Gram-negative bacteria to carbapenem antimicrobials is particularly relevant for patients with chronic kidney disease (CKD) who are vulnerable to these infections. General objective: To evaluate the prevalence of infections caused by gram-negative bacteria resistant to carbapenems in patients with chronic kidney disease and in kidney transplant patients. In addition, discuss the epidemiology of these infections, prevention and treatment strategies, as well as future directions in combating antimicrobial resistance. Method: This is a systematic review carried out according to Cochrane guidelines with extraction of data of interest and analysis of results carried out in accordance with the PRISMA statement (2022), seeking to assess the presence or development of infection caused by bacteria Carbapenem-resistant gram-negatives in patients with chronic kidney disease or patients on dialysis or kidney transplant patients. Results: After analysis, a total of 13 articles were included in this review: Pubmed (2), Web Of Science (5), ,Scopus (1) (7.7%) and Embase (5). published in the United States (30.7%), followed by Brazil (15%), South Korea, (7.7%) Israel, (7.7%) China, (7.7%) Spain, (7, 7%) Italy, (7.7%) Colombia (7.7%) and Greece (7.7%). Regarding the study design, 9 were retrospective cohorts, (69%), 3 were case control (23%) and 1 cross-sectional study (7.7%). The age range of the individuals ranged from 18 to 75 years (mean 46.5) and the follow-up time ranged from 6 months to 10 years. The bacterial identification methodology that prevailed among the analyzed articles was the automated VITEk2R (8 studies, 61.5%) and the most prevalent Gram-negative bacteria species were: Klebsiella pneumoniae (11/84.6%), Escherichia coli (6/46%), Pseudomonas aeruginosa 2 (15.4%), Citrobacter sp 2 (15.4%). ,Enterobacter sp 2 (15.4%), Morganella Morganii 1 (7.7%), Proteus mirabiliis 1 (7.7%) and Providência stuarti 1 (7.7%). The predominant sites of infection were the urinary tract 10 (77%) bronchoalveolar lavage, blood and feces with 1 (7.7%) site each and surveillance cultures were also reported (38.4%). For the detection of resistance to carbapenems, the molecular method (PCR 38.4%), modified Hodge method (23%), Kirby Bauer (15.4%) and CarbaNP (7.7%) were used in the studies, considering as reference the protocols of the Clinical and Laboratory Standards Institute (CLSI) (69%) and the European Committee on Antimicrobial Susceptibility Testing (Eucast) (7.7%). The bacterial resistance mechanisms found in bacterial isolates were production of inactivating enzymes: KPC (53.8%), NDM (7.7%) and VIM (7.7%), ESBL (15.4%) were also detected. As a clinical outcome, it was possible to observe that diabetic patients or those with a history of colonization by multidrug-resistant Gram-negative bacteria had a worse prognosis. The mortality rate ranged from 41.9% to 79% at 30 days, while in transplant patients infected with KPC-producing bacteria, the median survival was one month.

16
  • ANGELO ELIAS MERI JUNIOR
  • RELATIONSHIP BETWEEN THE USE OF DIFFERENT PHARMACEUTICAL PRESENTATIONS OF METFORMIN

    HYDROCHLORIDE AND THE OCCURRENCE OF GASTROINTESTINAL ADVERSE EVENTS: A BIOPHARMACEUTICAL

    AND USER PERCEPTION STUDY


  • Leader : WHOCELY VICTOR DE CASTRO
  • MEMBRES DE LA BANQUE :
  • WHOCELY VICTOR DE CASTRO
  • ANDRE DE OLIVEIRA BALDONI
  • ANA JULIA PEREIRA SANTINHO GOMES
  • JOSÉ EDUARDO GONÇALVES
  • MAIRON CESAR COIMBRA
  • Data: 15 déc. 2022


  • Afficher le Résumé
  • Introduction: Metformin hydrochloride (MET) is a hypoglycemic agent of oral administration, considered the drug of choice for the treatment of type 2 diabetes mellitus (DM2). The main adverse effects (AEs) related to the use of MET are gastrointestinal disorders. A hypothesis associated with the occurrence of these AEs due to the use of MET is related to the speed of drug release from its pharmaceutical form, since the higher the release rate, the higher its concentration in the intestine. It is relevant to investigate whether there is a difference in the perception of gastrointestinal AEs, as well as the correlation with the quality and the rate of drug release among the different commercial products containing MET. Methods: This is a prospective observational cohort study, in which diarrhea was related by patients with DM2 who started treatment with extended-release (XR) formulations of MET at doses of 500 mg. Additionally, at this stage of the study, the assay of MET and the test of uniformity of dosage units of different formulations of MET were conducted as described in the monographs of the American Pharmacopoeia. The dissolution profiles of the different MET brands were also performed in order to verify the percentage of the drug dissolved as a function of time. Results: Considering the patients eligible for study participation (n= 27) nineteen (66.7%) used the reference drug (R) and eight (33.3%) used the generic drug (G) of MET 500 mg XR. A lower frequency of gastrointestinal AEs was observed in patients who used the reference formulation when compared to those who used the generic. In both groups (R and G) the AEs did not affect the social and family life, as well as the quality of life of the patients. In the G4 sample, the assay test revealed that the content of MET was below the limit specified by the America Pharmacopoeia, being therefore considered an unsatisfactory result. The G2 sample presented the maximum amount of MET dissolved in the time of 10 minutes (97.69%), remaining constant subsequently. In G3, the amount of dissolved MET was gradual, reaching a maximum concentration in 25 minutes (92.07%), remaining constant afterward. The results obtained so far suggest that the AEs and the rate of drug release may be associated with the type of formulation, however, additional studies involving the evaluation of quality, dissolution profile, and the perception of AEs are still being performed in order to better support the hypothesis raised. It is expected that this work can contribute to the optimization of the pharmacotherapy of patients intolerant to gastrointestinal AEs resulting from the use of MET.

2021
Thèses
1
  • ALDA CRISTINA FRANCO CORREA MAIA
  • PHARMACOKINETIC MODELING AND PK / PD CORRELATION OF VANCOMYCIN


  • Leader : CRISTINA SANCHES
  • MEMBRES DE LA BANQUE :
  • ANA LEONOR PARDO CAMPOS GODOY
  • CRISTINA SANCHES
  • WHOCELY VICTOR DE CASTRO
  • Data: 30 mars 2021


  • Afficher le Résumé
  • Introduction: Although the criteria for monitoring drugs in critically ill patients are much clearer today, the proportion of patients receiving this service is still much lower than necessary in hospitals in Brazil. Monitoring through the principle of pharmacokinetics and pharmacodynamics (Pk / Pd) in critically ill patients aims to meet the needs of dose, concentration and ideal effect for the patient, with improvement in the clinical outcome and decrease in the likelihood of developing bacterial resistance. In this sense, this work aims to evaluate the different pharmacokinetic and pharmacodynamic profiles (PK / PD) of a population. Methods: This is a cross-sectional, documentary study. The research was based on information, the medical records of critically ill patients at the co-participant institution who used the drug vancomycin and who were subjected to drug monitoring during use. Individual patient data were collected from medical records. To determine the pharmacokinetic profiles of Vancomycin, as well as to model the population pharmacokinetics of this drug, the Bayesian tool was used, using the Monolix® software. To determine the pharmacodynamic profile, EUCAST data (European Committee for Antimicrobial Susceptibility Tests) with mic values of 0.125.0.25, 0.5, 1. Results: The vancomycin PK profile was well characterized by a one-compartment model with age covariates and serum creatinine, statistically influencing clearance (CL) and volume of distribution (V). The population clarence value was 1.52 L / h and the volume of distribution was 192.49 L. Of the dialysis patients, only 20% had full coverage for all Mics and of the patients without dialysis, 17% were fully protected. Conclusion: The pharmacokinetics of vancomycin in the population studied were significantly affected by creatinine and age. The pk / pd correlation across the area on the curve (AUC / MIC> 400) demonstrated that the standard doses administered were not able to guarantee the efficiency of treatment for all patients on dialysis or not.


2
  • MARIANA CAROLINY FERREIRA
  • ASSOCIAÇÃO ENTRE PARÂMETROS CLÍNICOS E LABORATORIAIS COM A QUALIDADE DE VIDA DE PACIENTES COM DIABETES MELLITUS TIPO 2

  • Leader : CAROLINE PEREIRA DOMINGUETI
  • MEMBRES DE LA BANQUE :
  • CAROLINE PEREIRA DOMINGUETI
  • MARIA DAS GRACAS CARVALHO
  • MARIANA LINHARES PEREIRA
  • Data: 6 mai 2021


  • Afficher le Résumé
  • The prevalence of diabetes mellitus has increased considerably worldwide,
    including in Brazil. The chronic nature and the need for lifelong treatment, in addition to
    comorbidities and complications associated with diabetes mellitus may result in compromised
    patients' quality of life (QOL). Objectives: To evaluate the association between knowledge,
    attitude and clinical, sociodemographic and laboratory variables with the quality of life (QOL)
    of patients with type 2 diabetes mellitus (DM2). Methods: A cross-sectional study was
    performed in 265 patients with clinical and laboratory diagnosis of DM2 who were treated at
    the Basic Health Units of Divinópolis -MG with data registered in the Integrated Health
    System (SIS). QOL, attitude, and knowledge were evaluated by validated questionnaires of

    summarized Brazilian Version of the Diabetes Quality of Life Measure Instrument (DQOL-
    Brazil-8); Brazilian Version of the Diabetes Knowledge Questionnaire Instrument (DKN-A)

    and Brazilian Version of the Attitude Questionnaire Instrument (ATT-19), respectively. In an
    interview, patients answered a socioeconomic and sociodemographic questionnaire. Clinical
    and laboratory data were collected through the Integrated Health System (SIS). The patients
    were distributed into 2 groups according to the QOL classification. Group 1 included (good,
    very good or excellent) quality of life and group 2, (regular or poor) quality of life. Data were
    compared between groups, considering significant p <0.005. Results: Among the 265
    interviewed patients, 53.6% (n = 142) had a good QOL, 31.7% (n = 84) very good, 10.9% (n
    = 29) regular or poor and 3.8% great (n = 10). Through the DKN-A questionnaire it was
    observed that most patients with DM2 included in this study have no knowledge about DM2
    (n = 202; 76.2%). Through the ATT-19 questionnaire, regarding attitude towards DM2, there
    was no statistically significant difference between the group of patients with regular or poor
    quality of life and the group with good, very good or excellent QOL. Related to the use of
    medications, hypoglycemic agents (p = 0.036) were used more frequently. Insulin is used (p =
    0.023) with less frequency in patients who have a good, very good or very good QOL
    compared to those who have a regular or poor QOL. As for laboratory tests, patients with
    regular or poor QOL presented higher values of HbA1c (p = 0.043), isolated albuminuria (p
    <0.001) and albumin / creatinine ratio (RAC) (p = 0.035), besides higher HbA1c frequency ≥
    7.0% (p = 0.044); HDL cholesterol ≤ 50 mg / dL (p = 0.045) and isolated albuminuria ≥ 174
    mg / L (p = 0.020) than patients with good, very good or excellent QOL. According to
    multivariate analysis, female patients (p = 0.043), younger at diagnosis (p = 0.022) and those
    who suffered a stroke (p = 0.020) had worse QoL. Conclusion: In this study, patients'
    knowledge and attitude towards DM2 were not significant in relation to QOL, but a
    significant impact was observed in individuals with less age at the diagnosis of DM2, female
    and who suffered a stroke.

3
  • KASSIA RODRIGUES MARIANO
  • Clinical attributions of the pharmacist in the Pharmacy scenario Hospitalar Brasileira in light of the Basel Declarations of the International Federation of Pharmacists.

  • Leader : ANDRE DE OLIVEIRA BALDONI
  • MEMBRES DE LA BANQUE :
  • RENATA ALINE DE ANDRADE
  • ANDRE DE OLIVEIRA BALDONI
  • JESSICA AZEVEDO DE AQUINO
  • MARIANA LINHARES PEREIRA
  • Data: 28 mai 2021


  • Afficher le Résumé
  • Introduction: The International Pharmaceutical Federation (FIP) with the objective of guiding
    and promoting the development of hospital pharmacy practice has fostered the development of
    Basel Statements. Currently, this consensus has 65 recommendations. Brazil still lacks studies
    based on Basel Statements, as an international standard, to assess hospital pharmaceutical
    practices, especially the clinical duties of pharmacists.
    Objective: Analyze the perception of
    the pharmacist in the Brazilian hospital pharmacy scenario in relation to his clinical attributions
    related to “Influences on Prescribing” and “Monitoring of Medicines Use” in face of The
    revised Basel Statements FIP.
    Methods: Brazilian hospitals of high and medium complexity
    were identified and had their respective e-mail addresses extracted through the National
    Register of Health Establishments (CNES) in 2016. Then, a questionnaire prepared following
    the 65 Basel Statements was sent via targeted e-mail to hospital pharmacists of all country. For
    the analysis of the fulfillment of the pharmacist's clinical duties, a descriptive analysis of the
    recommendations referring in the theme “Influences on Prescribing” and “Monitoring of
    Medicines Use” of the Basel Statements was carried out and the results were categorized in: a)
    accordance with the statements, b) do not answer or, c) did not answer.
    Results: The number
    of eligible hospitals was 2,054. Of this total, pharmacists from 111 (5.4%) hospitals returned
    the survey. These professionals had an average age of 34.9 years, an average time in hospital
    pharmacy of five years and nine years of graduates, most of them had the title of specialist (71
    - 64.0%). In relation to hospitals, most were from the Southeast (54 - 48.65%), of medium
    complexity (63 - 56.75%), and with a number of suitable pharmacists, according to the
    recommendation of SBRAFH (98 - 88.29%). For the recommendations of the theme
    “Influences on Prescribing” only in one recommendation the percentage of compliance was
    greater than 50%, corresponding to 54.1%. While for the “Monitoring of Medicines Use”, no
    recommendation was met by more than half of the hospitals.
    Final considerations: The low
    percentages of compliance with several declarations related to “Influences on Prescribing” and
    “Monitoring of Medicines Use” addressed by the FIP Basel Statements emphasize that these
    activities in the Brazilian hospital pharmacy need to be reviewed and aligned to offer better
    quality and more patient safety.


4
  • CARLOS ANANIAS APARECIDO RESENDE
  • Evaluation of Total Tannin Content in Fruit Pulps by Spectrophotometric Method

  • Leader : JOAQUIM MAURICIO DUARTE ALMEIDA
  • MEMBRES DE LA BANQUE :
  • ANA JULIA PEREIRA SANTINHO GOMES
  • JOAQUIM MAURICIO DUARTE ALMEIDA
  • NATHÁLIA LUCCA SILVA
  • RENE OLIVEIRA DO COUTO
  • Data: 2 juin 2021


  • Afficher le Résumé
  • The use of fruit pulps, leaves and peels generates products that can be useful for isolating complex molecules with application in nutraceutical supplements, dietary additives, new pharmaceutical and food products. The development of spectrophotometric methods for the quantification of phenolic compounds in food makes it possible to evaluate the quality of these natural products according to the active compounds they have. This study aimed to evaluate the levels of total tannins in fruits using a spectrophotometric method. In this work, the tannins were extracted using the turbo-extraction method, using different extractive solutions, and subsequently the determination of total phenolics and total tannins was performed using the Folin - Ciocalteau method. The fruit samples evaluated were: banana (Musa sp.), Guava (Psidium guajava) and grape (Vitis vinifera). The ethanol: water extraction process showed the highest yield in the extraction of tannins in most of the evaluated samples. However, the peel of P. guajava and the pulp of M. indica obtained the highest levels of tannins when the solvent system was composed of acetone: water. Thus, it was possible to conclude that the ethanol: water solvent system would be the solvent system of choice to obtain the highest levels of phenolic compounds in the fruits used in this study.

5
  • ADRIELLE PIEVE DE CASTRO
  • Resistance to carbapenems and investigation of the synergistic effect based on minocycline under concession of Acinetobacter baumannii

  • Leader : CRISTINA SANCHES
  • MEMBRES DE LA BANQUE :
  • CRISTINA SANCHES
  • JÚLIO CÉSAR MOREIRA DE BRITO
  • KARINA MARJORIE SILVA HERRERA
  • MAGNA CRISTINA DE PAIVA
  • MARIANA MARTINS GONZAGA DO NASCIMENTO
  • Data: 8 juin 2021


  • Afficher le Résumé
  • Acinetobacter baumannii is an important pathogen associated with health-related infections,especially in intensive care units. Hospital storms caused by carbapenem-resistant A. baumannii (CRAB) have been provided worldwide. Away from the rapid ability of this pathogen to acquire the mechanism of resistance to antimicrobials and the new treatment options, an association between different antimicrobials that were used as a strategy to improve an effective herd, especially in obtaining multidrug-resistant drugs (MDR). In this context, minocycline was identified as an option in therapeutic changes by A. baumannii due to its favorable safety profile, its ability to overcome various mechanisms of bacterial resistance and in vitro activity against A. baumannii MDR strains. This work aims to expand the knowledge of the context of bacterial resistance and to evaluate the in vitro activity of minocycline-based antimicrobial associations under CRAB concession. The guests, coming from hospitalized patients, had the profiles of susceptibility to antimicrobials determined and investigated the possible multifaceted activities of minocycline with meropenem, ciprofloxacin, polymyxin B and colistin (polymyxin E). All levels reinforced at a high level for carbapenems and ciprofloxacin. Polymyxin B, in turn, was the antimicrobial for which there was a higher percentage of susceptibility (91.3%) and, consequently, the one with the highest potency (MIC 50: 1 μg / mL). Synergism between miniocycline and meropenem was observed in most cases and the indifferent or antagonistic effect was observed mainly for an association between minocycline and polymyxins. This study showed that the association of a carbapenemic (meropenemic) with minocycline may be a strategy for the treatment of drugs by CRAB and, despite the high resistance to antimicrobials, observing the pathogens, a high susceptibility to polymyxin B.

6
  • LORENA AGUIAR SOARES
  • Implementation of a clinical pharmaceutical service in a hospital in the state of Minas Gerais

  • Leader : CRISTINA SANCHES
  • MEMBRES DE LA BANQUE :
  • CRISTINA SANCHES
  • FABIANA ROSSI VARALLO
  • PATRÍCIA MELO AGUIAR
  • Data: 29 sept. 2021


  • Afficher le Résumé
  • Introduction: The implementation of clinical pharmaceutical services in health institutions demonstrates a significant decrease in problems related to drugs. On the other hand, these services still lack a logical model for their implementation in a hospital environment. Objective: This work aims to elaborate and analyze the viability of a logical model, for the implementation of the clinical pharmaceutical service in a hospital environment. This is an implementation study carried out at Hospital Nossa Senhora da Conceição in the city of Pará de Minas in the state of Minas Gerais.

    Methodology: The implementation of clinical pharmaceutical services took place in three stages: Strategic management of the implementation of the service, definition and validation of the data collection instrument and pilot study. In the first stage, the project was elaborated and presented to the hospital manager, then a norm was elaborated, to be used to regulate the service in the hospital environment. Soon after the objectives were defined; short, medium and long term goals; work schedule; sector where each stage of the service will be performed. In the second stage, the data collection instrument was developed, divided into four blocks: 1) Analysis of the technical and legal aspects of the prescription, 2) Drug reconciliation, 3) Pharmacotherapeutic follow-up and 4) Hospital discharge. The data collection instrument was validated using the Delphi technique. The next step will be the incorporation of the pharmaceutical clinical form into the hospital's electronic system and then the pilot study. Results: The hospital manager approved the implementation of the project at the institution. The regulation was approved by the hospital's pharmacy and therapeutics committee and was published in Ordinance No. 01 of February 10, 2020. The actors involved in the implementation process were identified: ally, neutral and opposition. Activities related to the process of implementing the clinical pharmaceutical service were also presented. As a data collection instrument, the pharmaceutical clinical service form was developed and the Delphi Technique was used to validate it, which reached the consensus of the evaluators in the first round. Conclusion: Through this study, health institutions will have a technical framework to support the implementation of the clinical pharmaceutical service in a hospital environment, providing quality, safety for patients.

7
  • ISABELLA FERREIRA SILVA
  • Evaluation of the potential environmental risk of chloroquine diphosphate and hydroxychloroquine sulfate using the early development of Danio rerio as an experimental model.

  • Leader : HELIO BATISTA DOS SANTOS
  • MEMBRES DE LA BANQUE :
  • HELIO BATISTA DOS SANTOS
  • FARAH MARIA DRUMOND CHEQUER BALDONI
  • RALPH GRUPPI THOME
  • KINULPE HONORATO SAMPAIO
  • Data: 1 oct. 2021


  • Afficher le Résumé
  • Hydroxychloroquine sulfate (HCQ) and chloroquine diphosphate (CQ), despite their inefficacy, have been suggested as antiviral drugs in the fight against SARS-CoV2 in several countries, especially in Brazil.  In this sense, the increased consumption of these drugs for the treatment of COVID-19 may promote a potential environmental and public health risk. In this scenario, the objective was to evaluate the toxicity of sublethal concentrations of HCQ and CQ in Danio rerio fish embryos/larvae. The lethal concentration (LC50) of HCQ and CQ was calculated at 96 h. After obtaining the LC50 value, the toxicological assay was performed, in which embryos/larvae were subjected to sublethal concentrations of HCQ and CQ. Hatching rates, heartbeat frequency, and deformities in embryos/larvae exposed to the drugs were evaluated. The LC50 for HCQ was 560 µM and for CQ was 800 µM. Fish embryo acute toxicity (FET) assays were performed up to 96 h, in the semi-static system for HCQ and CQ. Thus, embryos/larvae exposed to sublethal concentrations of HCQ and CQ showed delayed hatching at 72 h and increased heart rate, edema in the pericardium and yolk sac area, and teratogenic effects such as crooked spine and shorter than normal tail at 96 h. The results show that HCQ and CQ are toxic to fish, especially in early development and understanding the mechanisms of toxicity, allowing us to extrapolate the adverse effects of these drugs when they reach the aquatic environment in the pandemic setting of COVID-19.

8
  • ANA ELISA MELO
  • ANALYSIS OF DRUG USE IN PATIENTS HOSPITALIZED BY COVID-19 IN A NEONATAL AND PEDIATRIC INTENSIVE CARE UNIT

  • Leader : MARIANA LINHARES PEREIRA
  • MEMBRES DE LA BANQUE :
  • Thaísa Amorim Nogueira
  • ANDRE DE OLIVEIRA BALDONI
  • MARIANA LINHARES PEREIRA
  • TATIANE DA SILVA DAL PIZZOL
  • Data: 8 oct. 2021


  • Afficher le Résumé
  •  In December 2019, in the city of Wuhan, China, a new type of coronavirus, named SARS-CoV-2, which causes COVID-19, was discovered. Its high transmissibility led the World Health Organization (WHO) to declare on March 11, 2020 a pandemic. The first case in Brazil was registered on February 26, 2020 and until September 29, 2021 infected 21,399,546 individuals and caused 596,122 deaths. In the pediatric population, most of the time, individuals are asymptomatic or have mild symptoms. Due to the lack of a therapeutic alternative for the treatment of the disease, the pandemic led to the use of off-label and unlicensed drugs, making the use of drugs less safe, facilitating the emergence of serious and unpredictable adverse reactions. The aim of this study is to describe the profile of patients hospitalized in a neonatal and pediatric intensive care unit and to evaluate the medications used in the treatment of COVID-19. The study design is cross-sectional with retrospective data collection from 02/26/2020 to 06/30/2021, collecting information from patients aged 12 years or less, admitted to the intensive care unit with a diagnosis of SARS-COV-2 infection (CID B342 and CID B972). The results showed that the largest number of patients were male infants, with a mean age of 0.26 years. The average length of stay was 5.9 days, with seven deaths as the outcome of hospitalization. In relation to prescribed drugs, all were considered off-label, when classified according to the Anatomical Therapeutic Chemical System (ATC), the highest prevalence was of electrolytes, antimicrobials/antifungals, analgesics and antiepileptics. Regarding the off-label drugs described as the most prevalent in the literature, we found prescriptions for Azithromycin, Oseltamivir and Salbutamol. Due to the scarcity of research and clinical trials, this study may contribute to understanding the profile of hospitalized patients and help to promote the effective and safe use of medicines in this challenging context.

9
  • MARINA VIEIRA
  • Cosmetics use for the management  of palm – plantar erythrodysesthesia (hand-foot syndrome) in cancer patients: a systematic review and data collection instrument validation

  • Leader : CARLOS EDUARDO DE MATOS JENSEN
  • MEMBRES DE LA BANQUE :
  • ANA LUIZA CHAVES MAIA
  • CARLOS EDUARDO DE MATOS JENSEN
  • MARCELO GONZAGA DE FREITAS ARAUJO
  • Data: 13 oct. 2021


  • Afficher le Résumé
  • Introduction: Câncer term is restricted to a single word that addresses more than 100 different types of malignant diseases, which have in common the disordered growth of cells, with ability to migrate and invade adjacent tissues or even distant organs. Disease capable of reaching and affecting populations in worldwide, regardless of age, gender, socioeconomic status, family arrangement or education. It is classified as a non-communicable chronic disease, and these presentes a global health problem, generating, in addition to a high number of premature deaths, a loss in life quality, associated with a limitation and incapacity degree. The cancer treatment focus is often restricted to the disease, with dermatological and self-image effects taking second place, in other words, cancer treatment has the primary objective in control and/or eliminating cancer cells. However, it is common that treatment be accompanied by adverse reactions related to the pathology itself or proposed treatment. Objective(s): To carry out an articles systematic review, with clinical approach, related to the prevention and/or management of  hand-foot syndrome, considering the cosmetic preparation use and to develop and validate an instrument to assess the patients life quality with erythrodysesthesia palmoplantar (hand-foot syndrome) with specialists. Materials and methods: The first stage of work was a systematic literature review in five different databases: Scopus, Web of Science, Science Direct, Pubmed and Virtual Health Library (VHL). Different Mesh descriptors were combined using a Boolean operator. The search and preparation of this review followed the guidelines proposed by PRISMA recommendation. Then, a data collection instrument was developed and submitted to ethics committee CAAE: 40485320.0.0000.5545 and later submitted to validation by judges. Results: The search strategy in databases and the selection process resulted in a total of 10 articles eligible for review. Among these studies selected so far, it was believed that the hand-foot syndrome was associated with capecitabine-based chemotherapy and that the effective syndrome clinical management was the chemotherapy dose interruption or reduction. In view of the results presented by study, hand-foot syndrome management with cosmetics was more advantageous than pharmacological management, with urea being chosen as the best moisturizer with an ability to delay syndrome development and to regress levels already installed. Conclusion: In all studies described in this review there was no need to discontinue chemotherapy and some presented cases of syndrome degree regression with cosmetics use, thus, cosmetic management, in addition to circumventing treatment side effect, also improves the patient's quality of life.

     

10
  • ALESSANDRA APARECIDA DE MELO SOUZA
  • Callus induction, cell suspension production and phenolic compounds evaluation in Tecoma stans (Bignoniaceae).

  • Leader : ANA HORTENCIA FONSECA CASTRO
  • MEMBRES DE LA BANQUE :
  • ANA HORTENCIA FONSECA CASTRO
  • GERALDO ALVES DA SILVA
  • LUCIANA ALVES RODRIGUES DOS SANTOS LIMA
  • Data: 21 oct. 2021


  • Afficher le Résumé
  • The culture of calluses and cell suspensions is an important biotechnological tool that can be used to obtain bioactive vegetable substances, by adding growth regulators to the culture medium. Tecoma stans or ‘‘garden ipe’’ is a grove native to tropical regions of North America, used in Brazil for ornamentation of urban centers. This plant has actions against oxidative stress, angiogenesis, lipid peroxidation and inflammation processes, effects associated with phenolic compounds, especially flavonoids present in flowers and leaves. In order to establish callus cultures and cell suspensions from Tecoma stans leaf segments and evaluate the production of phenolic compounds, leaf explants were inoculated in MS medium, supplemented with 30 g L-1 of sucrose and added with 2, 4-D (0; 4.52; 9.05; 18.10 μM) and BAP (0; 4.44; 8.88; 17.75 μM) and their different combinations and incubated in the presence and absence of light. After 45 days of inoculation, the callus induction and oxidation percentage, the color, consistency, dry and fresh matter of the calluses and the contents of total phenols and flavonoids were evaluated, using usual methodologies. The callus growth curve was established by inoculating new leaf explants in MS medium supplemented with 30 g L-1 of sucrose and added with 9.05 µM 2,4-D + 17.75 µM BAP, for a period 120 days. Cell suspensions were obtained from the inoculation of friable calluses in liquid WPM medium, containing 30 g L-1 of sucrose and 9.05 μM 2,4-D, under agitation at 80 rpm, in the absence of light. Cell growth was assessed by determining the sedimented cell volume (VCS). The results obtained showed that the calluses induced in medium supplemented with 9.05 µM 2,4-D + 17.75 µM BAP, in the absence of light, presented high induction rate (93%), low oxidation rate (20%), yellow color, friable consistency, considerable values of fresh and dry matter, high levels of phenols and the highest levels of total flavonoids (119.88 ± 9.34 µg EqAG mg-1 EB and 97.11 ± 12.81 µg EqR mg-1 EB), respectively. So far, the growth curve has shown a sigmoidal pattern, with five distinct phases: lag, exponential, linear and stationary and declining. The initial suspensions at 15 days of age showed higher VCS and, consequently, greater cell growth, when subcultured in WPM medium supplemented with half the concentration of 2,4-D, compared to those subcultured in WPM medium containing 9.05 µM 2,4-D. No significant cell growth was observed from initial suspensions at 21 and 28 days of age. These results showed the possibility of obtaining calluses and cell suspensions of T. stans and phenolic compounds in vitro from callus cultures.

11
  • LAYS CÁSSIA SANTOS DUARTE
  • Drug reconciliation: Interventions and avoided costs

  • Leader : ANDRE DE OLIVEIRA BALDONI
  • MEMBRES DE LA BANQUE :
  • MARIANA LINHARES PEREIRA
  • ANDRE DE OLIVEIRA BALDONI
  • RENATA ALINE DE ANDRADE
  • MAURILIO DE SOUZA CAZARIM
  • Data: 30 nov. 2021


  • Afficher le Résumé
  • a

12
  • ANNA CAROLINA DE MOURA COSTA


  • ESTUDO OBSERVACIONAL SOBRE AS APREENSÕES DE MACONHA,
    COCAÍNA E CRACK, EM UMA CIDADE DA REGIÃO METROPOLITANA DE
    BELO HORIZONTE

  • Leader : FARAH MARIA DRUMOND CHEQUER BALDONI
  • MEMBRES DE LA BANQUE :
  • ANA HORTENCIA FONSECA CASTRO
  • CRISTINA SANCHES
  • ELISA RAQUEL ANASTÁCIO FERRAZ AVELINO
  • FARAH MARIA DRUMOND CHEQUER BALDONI
  • Data: 1 déc. 2021


  • Afficher le Résumé
  • It is estimated that 275 million people in the world between 15 and 64 years old used
    some type of drug in the year 2020. In that same year, the Brazilian Federal Police
    seized about 546,439.57 kg of marijuana and 91,234.87 kg of cocaine (hydrochloride,
    crack and base paste), demonstrating that the use and abuse of psychoactive
    substances are on the rise in everyday life. The use drugs is highly linked to conditions
    of social vulnerability and increased crime rates, and for this reason, the city of Betim
    was the target of study, as it is considered one of the most violent in the state of Minas
    Gerais. Thus, this study aimed to describe the profile of arrest of marijuana, cocaine
    and crack, carried out by the Civil Police, in the region of Betim-MG, in 2017 and 2018.
    This is a descriptive observational study outlined, according to the guidelines proposed
    by the Declaration of Strengthening Reports of Observational Studies in Epidemiology
    (STROBE), carried out on a documentary basis from January 2017 to December 2018.
    The collection was divided into four stages: survey of the definitive reports issued to
    Police Stations in Betim in 2017 and 2018; PcNet: requisition conferences; PCNet:
    verification of definitive reports; and Social Defense Event Records (REDS): REDS
    analysis. The sociodemographic profile of individuals apprehended in connection with
    illicit drugs, the origin and locations of arrest, related crimes, the occurrence of
    apprehension of marijuana, cocaine and crack, and the ways in which these drugs
    were apprehended were determined. The database was created using Questionnaire
    Development System (QDS) version 2.6.1.1. Subsequently, data were exported to the
    Statistical Package for Social Sciences (SPSS) version 19, for statistical analysis. In
    total, 568 definitive reports and REDS were analyzed for the study period. From the
    analysis of these documents, the sociodemographic profile of the individual
    apprehended for a relationship with illicit drugs was mainly composed of men (86.4%),
    single (86.6%), with incomplete primary education (33.2%), and with a median age of
    20 years. The Police Station on Duty (88%) and the regional offices of the city of Betim,
    Norte (22.7%), Imbiruçu (18.4%) and Alterosas (15.6%) were where the greatest arrest
    occurred and in relation to crimes related to these apprehensions, for the most part,
    corresponded to illicit drug trafficking (85.6%). As for the type of drug, in both years, it
    was observed that marijuana was the drug arrest in the greatest amount (51.1%),
    followed by cocaine (29.6%) and crack (19.2%). The knowledge of the profile of drug
    arrest in this specific region (Betim-MG), allows preventive measures for the trafficking

    ix
    and use of illicit drugs to be created, contributing to the improvement of public health
    and safety.

13
  • Conceição Aparecida Lisbôa Coêlho
  • Development of an Analytical Method for The Determination of Lithium and a Study for Monitoring SUS Patients with Clinical Picture of Bipolar Disorder

     

  • Leader : FRANK PEREIRA DE ANDRADE
  • MEMBRES DE LA BANQUE :
  • VANESSA MOREIRA OSÓRIO
  • FRANK PEREIRA DE ANDRADE
  • RAFAEL CESAR RUSSO CHAGAS
  • Data: 8 déc. 2021


  • Afficher le Résumé
  • Aluminum ion is considered toxic for patients with chronic renal failure, especially for patients on hemodialysis due to exposure to the large volume of water used in preparing the dialysate. The Al3+ cation present in the dialysate can cross the dialyzer membrane into the bloodstream, and through transferrin be transported to various organs, mainly to the bones, lungs and brain, further weakening the renal patient's health. Currently, little is said about Al3+ intoxication problems in hemodialysis patients. The implantation of a water treatment system with reverse osmosis contributed a lot to avoid this type of problem, but the poisoning continues in a silent way through the accumulation of aluminum, mainly in the bones. One of the ways to increase safety is by monitoring Al3+ in the water used. Thus, the purpose of this work was to develop a technique by UV-VIS spectrophotometry, in order to quantify aluminum in water for hemodialysis, considering that this technique is not costly and can be easily performed. The method developed used the chromogenic reagent alizarin, 1,2-dihydroxy-9,10-anthraquinone – (DHA) to complex Al3+. A factorial design with central composite 23 was applied to obtain the best pre-concentration conditions by cloud point, optimized in an ultrasonic bath. The optimal reaction conditions were experimentally defined at pH 5,59 with biphthalate/KCl buffer, Al3+-DHA molar ratio of 1:4.1, cationic surfactant, benzalkonium chloride 9,4 µL  at 5 % m v–1 and sonication for 5 minutes. The sample was pre-concentrated by cloud point using 0.47% anionic surfactant (triton X–114). This process took place at a temperature of 60ºC on a heating plate. The absorbance reading was performed in a UV–VIS spectrophotometer at 520 nm, using 1 mL of the pre-concentrated sample and 5 drops of methanol. The linear range obtained was from 0.04 to 0.1 µg mL–1. The method presented adequate precision with CV less than 5% and adequate accuracy, with recovery in the range of 94.6 to 105.9%. The limits of detection and quantification were set at 0.006 and 0.02 µg mL–1 respectively.

     

     
14
  • Liliane Dias Campos
  • Development of an Analytical Method for The Determination of Lithium and a Study for Monitoring SUS Patients with Clinical Picture of Bipolar Disorder

     

  • Leader : FRANK PEREIRA DE ANDRADE
  • MEMBRES DE LA BANQUE :
  • FRANK PEREIRA DE ANDRADE
  • RENE OLIVEIRA DO COUTO
  • SILVÉRIA NEVES DE PAULA E SOUZA
  • WHOCELY VICTOR DE CASTRO
  • Data: 15 déc. 2021


  • Afficher le Résumé
  • Introduction: Bipolar affective disorder is a chronic multicomponent disease that involves episodes of severe mood disorders, neuropsychological deficits, immunological and physiological changes. Lithium carbonate is a more suitable mood stabilizer drug, but the one that can be more toxic. In search of an alternative to this impasse, analytical methods to measure the serum amount in patients would be ideal. Many techniques are already used today, but they are very costly. The UV-VIS methodology would be a simpler and more accessible option.

    Objectives: To develop an analytical method for the determination of serum lithium using UV-VIS spectrophotometry in order to enable a study for further therapeutic monitoring of patients treated by the Unified Health System (SUS) in Divinopolis, MG.

    Methods: The study was divided into 2 steps: 1) to develop and validate an analytical method for the determination of serum lithium in blood samples. Different complexants were tested at a concentration of 7.25 x10-3 mol L-1. The experiments were carried out using the thorin complexant, which presented a better analytical condition. The method was validated following the Eurachen and Inmetro guidelines. 2) to propose a study model to monitor serum lithium in patients with bipolar disorder, who are assisted by the SUS, residing in Divinópolis, MG.

    Results: Through a univariate optimization, a viable spectrophotometric method was developed to determine lithium in blood samples. The complexing agent used was Thorin 25% m v-1 and the linear working range was 0.2 to 2.0 μg/mL, which exhibited good precision, accuracy and sufficient sensitivity to allow the quantification of Lithium in patient samples. Eighty-five samples of patients using the drug lithium carbonate were analyzed by the method, and of these, only 6 were outside the therapeutic range, which showed that patients assisted in the SUS in Divinopolis have their serum dosages, relatively within the therapeutic drug range.

     
2020
Thèses
1
  • BRAYAN JONAS MANO SOUSA
  • -

  • Leader : JOAQUIM MAURICIO DUARTE ALMEIDA
  • MEMBRES DE LA BANQUE :
  • ANANDA LIMA SANSON
  • JOAQUIM MAURICIO DUARTE ALMEIDA
  • WHOCELY VICTOR DE CASTRO
  • Data: 10 févr. 2020


  • Afficher le Résumé
  • -

2
  • BRUNA CRISTINA ALVES
  • -

  • Leader : JOAQUIM MAURICIO DUARTE ALMEIDA
  • MEMBRES DE LA BANQUE :
  • JOAQUIM MAURICIO DUARTE ALMEIDA
  • MAIRON CESAR COIMBRA
  • MARLUCIA SOUZA PADUA VILELA
  • Data: 14 févr. 2020


  • Afficher le Résumé
  • -

3
  • MARIANA ALVES REZENDE SALGADO
  • -

  • Leader : HELIO BATISTA DOS SANTOS
  • MEMBRES DE LA BANQUE :
  • AMANDA LUISA DA FONSECA
  • HELIO BATISTA DOS SANTOS
  • MARIANA LINHARES PEREIRA
  • RALPH GRUPPI THOME
  • Data: 17 févr. 2020


  • Afficher le Résumé
  • -

4
  • KARINA APARECIDA RESENDE
  • Competência de julgamento clínico de estudantes de farmácia em final de formação por meio da simulação realística em saúde

  • Leader : ANGELITA CRISTINE DE MELO
  • MEMBRES DE LA BANQUE :
  • AFONSO MIGUEL DAS NEVES CAVACO
  • ANA ANGELICA LIMA DIAS
  • ANGELITA CRISTINE DE MELO
  • MARTA MARIA DE FRANCA FONTELES
  • Data: 28 févr. 2020


  • Afficher le Résumé
  • Introduction: In the provision of pharmaceutical care, clinical judgment is essential. Professionals with
    good performance in this competence tend to achieve better results in health care. The pharmacist&#39;s
    training process is crucial for the development of this competence. Objectives: To analyze the
    competence of clinical judgment in the provision of care in primary health care by Brazilian students of
    the ninth and tenth undergraduate courses in pharmacy through the use of health simulation, with
    simulated patients. Methods: It is a descriptive and multicenter study with twenty-two students from the
    ninth and / or tenth periods of the undergraduate course in Pharmacy from each collaborating center.
    The study method consisted of different stages, namely: study design and sample recruitment, definition
    of learning objectives, development of the scenario and measurement instruments, pilot test of the
    scenario, training and standardization of the simulated patients using the Masp instrument, guarantee of
    student preparation, prebriefing, briefing, debriefing, standardization of judges and analysis of clinical
    judgment using the instrument Lasater Clinical Judgment Rubric - Brazilian Version. Results: The
    training and standardization of the simulated patients was developed with good reliability and
    reproducibility. The internal consistency was estimated using Cronbach&#39;s alpha and the limits of 0.70 and
    0.90 were considered acceptable. Correlations were assessed by the interclass correlation coefficient,
    considered poor if &lt;0.4; satisfactory to good if 0.4 &lt;ICC &lt;0.75; excellent if ≥ 0.75 acceptable. The values
    of alpha of cronbach in the analysis of the patients varied between (0.699 to 0.978). The same was
    observed for the interclass correlation coefficient (0.424 to 0.978) indicating good agreement between
    the evaluators. The standardization process of the judges reached values of alpha of cronbach that
    varied between 0.879 and 0.933, indicating high reliability of the instrument Lasater Clinical Judgment
    Rubric - Brazilian Version. In relation to clinical judgment, 21.0% of the students were classified as
    beginners, 60.0% were in development, 17% proficient and only 2% were classified as exemplary. There
    is still a difference in the profile of clinical judgment between universities. Conclusion: The training and
    standardization method for simulated patients developed in this study showed high reliability and can be
    used in multicenter studies and evaluated its validity and reliability. The judges were standardized and
    reached a high level of agreement in the evaluation, inferring that it is possible to obtain the
    reproducibility of the Lasater Clinical Judgment Rubric - Brazilian Version instrument in the pharmacy
    area. The research contributed to the understanding of the clinical judgment of pharmacy students and
    showed that the level of performance is predominantly in development, which points to the need for
    progress in this area. The contribution of these results to the knowledge of clinical judgment processes
    can collaborate with adjustments in teaching, and the elaboration of proposals for improvement in the
    training of future professionals who provide pharmaceutical care to patients, families and the community.

5
  • DÉBORAH FERNANDES RODRIGUES COSTA
  • Bixina: obtenção, caracterização e avaliação da atividade antimicrobiana.

  • Leader : CARLOS EDUARDO DE MATOS JENSEN
  • MEMBRES DE LA BANQUE :
  • ELIANA DE FARIA GARCIA HORTA
  • CARLOS EDUARDO DE MATOS JENSEN
  • MAGNA CRISTINA DE PAIVA
  • MARIA DAS GRACAS CARVALHO
  • RENE OLIVEIRA DO COUTO
  • Data: 22 juin 2020


  • Afficher le Résumé
  • Bixin is an apocarotenoid found on the surface of annatto seeds (Bixa orellana L.).
    Can be used as coloring in various foods, in the textile industry and in cosmetics, in
    addition to having medicinal properties, including antimicrobial activity. However, this
    substance is still little explored. In this sense, the present study aimed to extract bixin
    from annatto seeds, characterize it by means of physical-chemical techniques,
    develop and validate an analytical method for quantifying this substance and
    evaluate its antimicrobial activity in vitro. Bixin was extracted through the sohxlet
    extractor using hexane and chloroform as solvents and, subsequently recrystallized
    in acetone. Bixin was characterized by thermal, infrared and nuclear magnetic
    resonance analysis. The analytical method for quantification was developed by high
    performance liquid chromatography with visible detection and validated according to
    national and international guidelines. In vitro antimicrobial activity was evaluated
    against ATCC strains of different species of Candida and Gram-positive and Gram-
    negative bacteria. Initially, the disk-diffusion method was followed by plate
    microdilution, both recommended by Clinical &amp; Laboratory Standards Institute (CLSI).
    For the strains that showed the best results, the minimum fungicidal concentration
    (MFC) and the death kinetics were determined. In addition, bixin activity was
    assessed against Candida virulence, cytotoxicity and selectivity factors. Bixin showed
    an endothermic fusion signal at 185.54 ºC, infrared bands and NMR signals
    compatible with its molecular structure and was identified in 5.950 minutes of
    analysis by the proposed analytical method, which showed suitability, selectivity,
    linearity (R²: 0. 9993), precision (DPR: 0.59) and accuracy (99.4% - 100.8%) and low
    limits of detection (0.638 µg mL -1 ) and quantification (1.934 µg mL -1 ). The best
    antimicrobial activity was identified for yeasts of the genus Candida, especially in C.
    glabrata ATCC 2001 and C. tropicalis ATCC 28707. MFC was compatible with the
    killing curve, indicating possible fungal activity of bixin in high concentrations (1,024
    µg mL - 1 ). Bixin has been shown to inhibit the yeast-hypha transition and was effective
    in disrupting mature Candida biofilm, has dose-dependent cytotoxicity and promising
    selectivity index for fungal cells to the detriment of mammalian cells. Therefore, bixin
    can be efficiently extracted and quantified from the proposed methods and has
    antifungal potential, indicating that further studies should be carried out with a view to
    possible therapeutic use.

6
  • LUANNA GABRIELA RESENDE SILVA
  • Efetividade de um protocolo de desprescrição de Clonazepam em idosos: estudo piloto.

  • Leader : ANDRE DE OLIVEIRA BALDONI
  • MEMBRES DE LA BANQUE :
  • ANDRE DE OLIVEIRA BALDONI
  • ANA CRISTINA DE LIMA PIMENTEL
  • CLARECI DA SILVA CARDOSO
  • CARINE RAQUEL BLATT
  • Data: 1 juil. 2020


  • Afficher le Résumé
  • -

7
  • MARCO TÚLIO ALVES SANTOS
  • EFEITOS DOS DERIVADOS DA DIGOXINA BD-13 E BD-14 NA MIGRAÇÃO E ADESÃO CELULAR

  • Leader : LEANDRO AUGUSTO DE OLIVEIRA BARBOSA
  • MEMBRES DE LA BANQUE :
  • GUSTAVO BLANCO
  • LEANDRO AUGUSTO DE OLIVEIRA BARBOSA
  • RALPH GRUPPI THOME
  • RUBÉN GERARDO CONTRERAS PATIÑO
  • Data: 4 nov. 2020


  • Afficher le Résumé
  • --

2019
Thèses
1
  • BRUNA GABRIELA ROCHA
  • -

  • Leader : CRISTINA SANCHES
  • MEMBRES DE LA BANQUE :
  • CRISTINA SANCHES
  • FABIANA ROSSI VARALLO
  • GLAUCIA MARIA LOPES REIS
  • MARIANA LINHARES PEREIRA
  • Data: 29 nov. 2019


  • Afficher le Résumé
  • Introduction: Thinking of standardizing pharmaceutical services in hospitals around the world, professionals who work at the International Pharmaceutical Federation (FIP) gathered and drafted the Basel Statements, which cover all areas of the drug use process in hospitals. This is the first study conducted to analyze the perception of pharmacists regarding compliance with the Basel Statements by the Brazilian Pharmacy Hospitalar. Methods: Data on Brazilian hospitals of high and medium complexity and their respective electronic contact addresses were acquired through the Brazilian National Database of Healthcare Facilities (CNES).Then, a prepared questionnaire following the 65 Basel statements of 2015 was emailed to hospital pharmacists across the country. For the analysis of compliance with the activities of the Brazilian hospital pharmacy, the domains were grouped into areas of Pharmaceutical Care, Human Resources and Patient Safety. All statistical analyzes were performed by IBM SPSS v.19. Results: A total of 111 pharmacists from hospitals in all regions of Brazil answered the questionnaire. Of these, most had a specialist title (n = 71; 64.0%), with a median time since graduation of nine years and five years of acting as responsible hospital pharmacist. Most hospitals were from the Southeast region (n = 54; 48.7%), of medium complexity (n = 65, 67.7%), and with adequate number of pharmacists, according to SBRAFH (n = 56; 50.5%). The requirements considered important for the evaluated domains were not met in most hospitals and the Brazilian hospital pharmacist still spends most of their time working with drug management activities. Conclusion: The low percentages of compliance with various statements emphasize that the activities of the Brazilian hospital pharmacy need to be reviewed and aligned to offer greater quality and safety to the patient. And this requires behavioral changes, not only from the pharmacist, but also from the establishments, representative bodies and educational institutions.

     

     

     
2
  • ARI SOARES DE OLIVEIRA NETO
  • ATIVIDADE ANTIFÚNGICA DE ATORVASTATINA IN VITRO E IN VIVO: REPOSICIONAMENTO DO FÁRMACO PARA O TRATAMENTO DE MICOSES MUCOCUTÂNEAS

  • Leader : MARCELO GONZAGA DE FREITAS ARAUJO
  • MEMBRES DE LA BANQUE :
  • JAQUELINE MARIA SIQUEIRA FERREIRA
  • MARCELO GONZAGA DE FREITAS ARAUJO
  • RODRIGO CASSIO SOLA VENEZIANI
  • Data: 13 déc. 2019


  • Afficher le Résumé
  • -

SIGAA | NTInf - Núcleo de Tecnologia da Informação - | Copyright © 2006-2024 - UFSJ - sigaa04.ufsj.edu.br.sigaa04