Banca de QUALIFICAÇÃO: JAIRO BATISTA NETTO

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
DISCENTE : JAIRO BATISTA NETTO
DATA : 27/02/2020
HORA: 14:00
LOCAL: Campus Centro Oeste D. Lindu - UFSJ
TÍTULO:

EVALUATION OF THE ANTINEOPLASTIC POTENTIAL OF THE COMBINATION OF MATTEUCINOL AND TEMOZOLAMIDE IN GLIOBLASTOMA LINES

PALAVRAS-CHAVES:

 

 

Glioblastoma, Experimental pathology, temozolomide, glioblastoma, Mateucinol

PÁGINAS: 45
GRANDE ÁREA: Ciências da Saúde
ÁREA: Farmácia
SUBÁREA: Farmacognosia
RESUMO:

Malignant brain tumors are a rare occurrence, accounting for approximately 2% of all cancers in adults. Despite the low frequency, these tumors have a high mortality rate. The most common primary neoplasms of the Central Nervous System (CNS) are those of glial origin, of these, glioblastoma (GBM) is the most prevalent, being also the most aggressive. Its histopathological characteristics present it as a grade IV neoplastic process, derived from astrocytes, with high rates of microvascular proliferation, tumor necrosis, tissue invasion, but with a limited capacity to promote metastases outside the CNS. The mortality rate remains significant, despite major advances in molecular biology, immunology and chemotherapy. Plant-derived products have historically offered compounds for the development of new and effective chemotherapeutic agents. In view of this immeasurable challenge, this study aimed to evaluate the therapeutic potential of the combination of the compound Matteucinol (Matt), derived from the species Miconia chamissois Naudin, associated with the gold standard chemotherapy for the treatment of GBM, temozolamide (TMZ). It was demonstrated that the Matteucinol / temozolamide (Mat-TMZ) combination showed selectivity, with cytotoxic action for the GBM U251 cell line, sparing NHA astrocyte cells. As observed by Zimograma, Mat-TMZ decreased the activity of metalloproteinase 2. Associated with this, we found that the treatment did not act on the adhesion of U251 cells to the matrix. A specific study with acridine orange and propidium iodide suggested that one of the mechanisms of death involved in the treatment of U251 cells with the combination (Mat-TMZ) is apoptosis, since the formation of microbubbles was observed, indicating nuclear fragmentation. In addition, the antitumor and anti-angiogenic evaluation, in vivo, by the chicken choriolantoid membrane (CAM) assay was performed and indicates that the compound presents itself as a possible treatment option for GBM

MEMBROS DA BANCA:
Interno - 2875448 - RALPH GRUPPI THOME
Interno - 1689019 - RAFAEL CESAR RUSSO CHAGAS
Externo à Instituição - DANIEL MOREIRA DOS SANTOS - FUNED