Banca de QUALIFICAÇÃO: VINICIUS SOUZA TARABAL

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
DISCENTE : VINICIUS SOUZA TARABAL
DATA : 17/08/2020
HORA: 13:30
LOCAL: google meet
TÍTULO:

 IMPACT OF DMPEI ON ANTI-ADHESIVE BIOFILM ACTIVITY IN BIOMATERIALS AND ITS BIOTECHNOLOGICAL APPLICATION AS BIOCIDE IN PIGMENTARY PREPARATIONS

PALAVRAS-CHAVES:

DMPEI, anti-adhesion, biofilm, catheters

PÁGINAS: null
GRANDE ÁREA: Ciências Biológicas
ÁREA: Bioquímica
RESUMO:

Hospital infections can occur due to the insertion of medical devices such as urinary catheters, which allow the migration of microorganisms from the external environment to the patient's organism, as well as the presence of microorganisms on inanimate surfaces in the hospital, such as walls, cabinets, handrails, door handles. and taps. These two routes of hospital infection have as common characteristics surfaces that favor cell adhesion and proliferation and formation of. Therefore, new technologies are being developed in order to modify surfaces so that the adhesion and survival of microorganisms is prevented or hindered. Among the molecules with biofilm anti-adhesive property and the ability to coat surfaces, DMPEI stands out. DMPEI is a synthetic polymer capable of coating surfaces without covalently bonding to it, with a high anti-adhesive biofilm capacity. In the present work, we coated siliconized latex catheter surfaces with DMPEI and evaluated the biofilm anti-adhesion of the coated catheter against Escherichia coli, Staphylococcus aureus and Candida albicans. The catheter coated with DMPEI showed anti-adhesion of biofilm of 82.74%, 88.10% and 92.69% for E. coli, S. aureus and C. albicans, respectively. The wettability of the coated and uncoated catheter was also assessed. The contact angle decreased from 50.48º to 46.93º on the surfaces of latex catheters treated with the polymer DMPEI, making them more hydrophilic. We developed two paint samples (A and B) for wall covering and one varnish (C) for covering cabinet, handle, tap and handrail, all containing DMPEI. The anti-adhesion of Staphylococcus aureus was evaluated against samples of paints and varnishes in different concentrations. Sample A showed 88.63%, 90.40% and 91.03% biofilm anti-adhesion at concentrations of 0.2; 2 and 20 mg.mL-1, respectively. Sample B presented 89.77%, 90.34% and 91.31% of biofilm anti-adhesion at concentrations of 0.2; 2 and 20 mg.mL-1, respectively. Sample C showed 88.11%, 90.06% and 90.91% biofilm anti-adhesion at concentrations of 0.2; 2 and 20 mg.mL-1, respectively. We evaluated the biofilm anti-adhesion of samples A, B and C over 3 weeks. During that time, biofilm anti-adhesion did not decrease. The DMPEI polymer has the potential to coat medical devices and inanimate surfaces with high biofilm anti-adhesion.

MEMBROS DA BANCA:
Presidente - 1675931 - PAULO AFONSO GRANJEIRO
Externo ao Programa - 435055 - JOSE ANTONIO DA SILVA
Externo à Instituição - ADRIANO GUIMARAES PARREIRA - UEMG