Banca de DEFESA: LUISA FERREIRA DA CRUZ

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : LUISA FERREIRA DA CRUZ
DATE: 02/12/2022
TIME: 08:00
LOCAL: https://meet.google.com/jih-ggck-xee
TITLE:

Prospection of lactic acid bacteria from different origins and evaluation of probiotic potential

KEY WORDS:

Prospecting, probiotic, lactic bacteria.

PAGES: 74
BIG AREA: Multidisciplinar
AREA: Biotecnologia
SUMMARY:

Probiotic bacteria are known for their ability to bring benefits to the host, such as maintaining the immune system, inhibiting the growth of pathogenic microorganisms and maintaining emotional well-being, and therefore, there is a growing interest in new lactic acid bacteria with probiotic potential. The aim of this study was to screen probiotic lactic acid bacteria from different sources and evaluate the probiotic potential. In vitro probiotic tests included survival under low pH and bile salts, cell surface hydrophobicity, autoaggregation, coaggregation, antibacterial activity, and safety properties were evaluated based on the antibiotic resistance profile. Forty-six bacteria with characteristics of lactic acid bacteria were isolated, 32 of which were discriminated as genetically different through the RAPD technique, which were isolated from the sources, kefir (n=9), cheese (n=4), kombucha (n=2), silage (n=3) and newborn feces (n=14). Most strains were resistant to simulated gastric acidity and bile salts. High hydrophobicity was observed for four strains. Autoaggregation ranged from 9.31-62.24% and the ability to coaggregate demonstrated a wide range of variation in coaggregative phenotypes. In addition, the strains also showed varying levels of resistance to clinically important antibiotics. Comparison between sources demonstrated that lactic acid bacteria isolated from different sources may have probiotic properties. In general, the evaluation of the probiotic properties of a large set of isolated lactic acid bacteria was essential to analyze the phenotypic characteristics of the isolates and verify that, regardless of the source of isolation, a strain can be considered probiotic. We conclude that the strains analyzed from different sources showed probiotic potential with potential application in industrial sectors, with the most promising strains being K8, QV1, SL4, FE2 and FPc4

BANKING MEMBERS:
Presidente - 1675931 - PAULO AFONSO GRANJEIRO
Interno - 1757978 - DANIEL BONOTO GONCALVES
Externa ao Programa - 1945166 - MAIRA DE CASTRO LIMA
Externa ao Programa - 1719911 - JULIANA TEIXEIRA DE MAGALHAES
Externo à Instituição - ANDRE LUIS MORAIS RUELA - UFOP
Externo à Instituição - ADRIANO GUIMARAES PARREIRA - UEMG