Banca de DEFESA: MICHELLI DOS SANTOS

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : MICHELLI DOS SANTOS
DATE: 31/05/2023
TIME: 08:30
LOCAL: Plataforma Google meet
TITLE:

Development of an immunological diagnostic kit for Schistosoma mansoni

KEY WORDS:

Schistosoma mansoni; chimeric proteins; diagnostic kits.

PAGES: 114
BIG AREA: Multidisciplinar
AREA: Biotecnologia
SUMMARY:

Schistosomiasis, a neglected tropical parasitic disease caused by trematode worms of the genus Schistosoma, affects approximately 240 million people worldwide. Diagnosis can be performed by parasitological, molecular, and immunological methods. In order to effectively control and monitor schistosomiasis, new diagnostic methods are essential. Due to the long time needed to prepare crude antigens for the immunodiagnosis of schistosomiasis, the preparation of recombinant antigens has become a promising alternative. In this sense, due to the need to develop a test with high sensitivity and specificity, the objective of this work is to optimize the conditions of an ELISA test for the diagnosis of schistosomiasis mansoni, with a view to technology transfer. In this context, using a rationally designed recombinant protein, the rSH1 protein, which was expressed in Escherichia coli BL21 (λDE3) pLysE and pLysS cells, was purified and used in an immunoassay. The maximization of the expression  processes was done through the Response Surface Methodology and the protein yield was from 0.034mg/mL to 1.234 mg/mL. The results suggest that the rSH1 protein is able to identify positive sera for schistosomiasis, however, it is still necessary to optimize parameters to determine the diagnostic accuracy of the assay. Thus, the rSH1 protein evaluated in this study has the potential to be used as a biotechnological input in the development of new strategies for the diagnosis of schistosomiasis.

BANKING MEMBERS:
Presidente - 1367304 - ALEXSANDRO SOBREIRA GALDINO
Externa ao Programa - 1889198 - LETICIA FERNANDES DE OLIVEIRA
Externo à Instituição - RONALDO ALVES PINTO NAGEM - UFMG
Externa à Instituição - GRASIELE DE SOUSA VIEIRA TAVARES - UFMG
Externa à Instituição - DENISE DA SILVEIRA LEMOS - UNIFENAS