Banca de QUALIFICAÇÃO: LUCAS SANTOS AZEVEDO

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : LUCAS SANTOS AZEVEDO
DATE: 29/06/2023
TIME: 13:30
LOCAL: CCO Dona Lindu
TITLE:

Phytochemical study, evaluation of antioxidant, allelopathic, and larvicidal activities, and cytotoxic, genotoxic, and antigenotoxic effects of Smilax fluminensis Steud. (Smilacaceae) seeds

KEY WORDS:

japicanga, antioxidant activity, alelopathy, herbicide

PAGES: 86
BIG AREA: Multidisciplinar
AREA: Biotecnologia
SUMMARY:

From Brazil, Smilax fluminensis Steud. belongs to the Smilacaceae family, and is popularly known as japicanga. Although this genus is well studied, the biological activities of S. fluminensis are poor studied. Therefore, this study aimed to characterize the extract and fractions of S. fluminensis, and evaluate the antioxidant, toxicological, and allelopathic activities. The ethanolic extract (EE) obtained by percolation was fractionated by liquid-liquid partition with hexane (FHEX), dichloromethane (FDCM), ethyl acetate (FACE), and the last phase was also considered (FHID). The phytochemical analysis of FHEX was performed with GC-MS and the other samples with HPLC-DAD-MS. Quantification of total phenolic compounds (TPC), total flavonoids (TF), total tannins (TT), FRAP and DPPH were evaluated in vitro with a spectrophotometer. Toxicity was verified by the Artemia salina Test (AST), and the analysis of germination and seedling growth was performed in vitro with A. cepa and L. sativa. The structural elucidation of FHEX identified fatty acids, with high concentration of ethyl linolenate, ethyl palmitate and ethyl linoleate. All polar samples exhibited derivatives of quercetin, luteolin, kaempferol and saponins. Antioxidant quantifications and analyses showed the highest yields (CFT: 44.29 µg EAG/mg, FT: 87.54 µg EQ/mg and TT: 31.72 µg EAG/mg) and lower EC₅₀ values (DPPH: 8.18 µg/ml and FRAP: 2.35 µg/ml) in FACE. TAS did not determine LD₅₀ in any sample. In seeds, all samples decreased the vigor, the germination rate and the GSI in A. cepa. Differently, they did not alternate the vigor and the germination rate in L. sativa, but they decreased the GSI, except FHID. In seedlings, all samples inhibited epicotyl and radicle growth of A. cepa and L. sativa, except FHEX increased epicotyl (750 µg/mL) and radicle (750 and 1000 µg/mL) growth.

BANKING MEMBERS:
Presidente - 1540495 - LUCIANA ALVES RODRIGUES DOS SANTOS LIMA
Interna - 395916 - ANA HORTENCIA FONSECA CASTRO
Externa à Instituição - THAIZ RODRIGUES TEIXEIRA - UCSD
Externa à Instituição - SIMONY CARVALHO MENDONÇA - UFRJ