Banca de DEFESA: JOSE ANTONIO RIBEIRO NETO

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : JOSE ANTONIO RIBEIRO NETO
DATE: 17/12/2020
TIME: 13:30
LOCAL: Campus Centro Oeste D. Lindu - UFSJ
TITLE:

"Eriosema pycnonthum (Benth), evaluation of the biotechnological potential in healing processes"

KEY WORDS:

Eriosema pycnanthum, in vitro, Ethnobotany, Ethnopharmacology, Healing

PAGES: 197
BIG AREA: Ciências da Saúde
AREA: Farmácia
SUBÁREA: Farmacognosia
SUMMARY:

The genus Eriosema, composed of 150 species, in nine taxa has its occurrence between Africa and the Americas. Eriosema pycnanthum is a legume from the Fabaceae family, a component of the Phaseoleae tribe and the Cajaninae subtribe. In Brazil, studies involving the genus Eriosema are limited to the description of new taxa or the occasional registration in surveys of regional flora. The present work aims to evaluate the healing action of the hydroalcoholic extract of E. pycnanthum, in addition to evaluating the plant structure, promoting the in vitro multiplication of the species and its replanting in a natural environment. The survey on the use of medicinal plants, especially E. pycnanthum, in the region of Itapecerica, Minas Gerais, was submitted and approved by CEPES-UEMG under number 2,812,199, where the free and spontaneous adoption of the popular to the treatment with E. pycnanthum was recorded through a questionnaire, monitoring, and photos of the evolution of the condition. Scatter maps were constructed from works and records of E. pycnanthum exsiccatae deposits across the territories of the Americas, Minas Gerais and Itapecerica. The multiplication of the species in vitro demonstrates low responsiveness of explants to conventional treatments. In a bioreactor, the increase in root mass occurs slowly, requiring improvements in the system and means of cultivation with elicitation. The low germination rate of its seeds is partly due to the rapid drop in respiratory efficiency, as evidenced by the Tetrazolium Bromide test, in addition to tegumentary numbness, proven after manual removal of the seed coat. The analysis with the DCCR-USP software shows that treatment with a 2% KNO3 solution is ineffective in improving these rates. The anatomical analysis shows amphistomatic leaves, paracitic stomata, Dorsiventral mesophyll with prevalent single-palisade parenchyma, vascular bundles concentrated close to the main vein without major changes between ex vitro and in vitro leaves, which, however, have differences in the continuity of the paranerval parenchyma. The seeds have no bad oils, have ample starch reserves and are equipped with 9 cell layers concentrated on the embryo. The extract composition analyzed by CCD of the field root, in vitro and tea, shows phytosterols such as β-sitoterol, β-Escina and Stigmasterol in addition to phenols such as Cinnamic Acid, Catechin, Rutin and Quercetin. The content of total phenols, flavonoids, phytosterols and alkaloids are higher in root extracts in vitro. NMR shows similar spectra of the composition of xi bad solutions with higher concentrations in root extracts in vitro and reveals the presence of flavonol, anthocyanin and sugar groups in addition to anomeric and glycosidic compounds. The hydroalcoholic extract of the field roots has a proliferative action on fibroblast and keratinocyte lines in vitro without the presence of cytotoxic action on normal lines. The replacement of water by tea from the field roots in animal tests, induces an improvement of the condition, especially during the inflammatory phase of the healing process.

BANKING MEMBERS:
Presidente - 1680611 - ROSY IARA MACIEL DE AZAMBUJA RIBEIRO
Interna - 395916 - ANA HORTENCIA FONSECA CASTRO
Externo ao Programa - 1716137 - HELIO BATISTA DOS SANTOS
Externa à Instituição - ANA GABRIELA SILVA
Externo à Instituição - GERALDO ALVES DA SILVA - UNIFAL-MG