Banca de DEFESA: BRUNA CRISTINA ALVES

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : BRUNA CRISTINA ALVES
DATE: 28/02/2024
TIME: 08:30
LOCAL: Campus Centro Oeste Dona Lindu
TITLE:

CULTIVATION OF CANNABIS SATIVA L. IN A CONTROLLED SYSTEM FOR THE PRODUCTION OF CANNABINOIDS

KEY WORDS:

Cannabis, auxins, pH, cannabinoids

PAGES: 90
BIG AREA: Multidisciplinar
AREA: Biotecnologia
SUMMARY:

The objective of this work was to analyze different treatments in the in vitro cultivation of Cannabis sativa L., from germination to seedling propagation. In the seed germination stage, a disinfestation test was carried out with the seeds. After this process, the seeds were inoculated in different culture media, with and without the influence of light for the germination analysis, in addition to the application of scarification tests to break dormancy. It was concluded that the use of fungicide does not influence the disinfestation process, being effective only the use of sodium hypochlorite and 70% ethanol. Physical scarification is efficient in overcoming dormancy, reducing germination time. In 36 hours, germinated seeds were already observed, compared to the control group. In addition, the loss of seed viability was observed in long periods of storage at room temperature. In the second stage of the work, tests were carried out for the propagation of the seedlings. Nodal segments of seedlings in vitro were inoculated in MS culture medium, with different concentrations of auxins, AIA, AIB and ANA (0.5, 1.0 and 2.0 mg/L) and also, in MS culture medium with pH variations, in order to verify the multiplication capacity of the explant. The best results in seedling development were obtained using MS medium with IBA, especially at the lowest concentration (0.5 mg/L). The pH 5.8 favored the growth of plants in vitro and with better root development.

BANKING MEMBERS:
Presidente - 1908201 - JOAQUIM MAURICIO DUARTE ALMEIDA
Externa ao Programa - 1080217 - FARAH MARIA DRUMOND CHEQUER BALDONI
Externa ao Programa - 3228039 - FERNANDA MARIA POLICARPO TONELLI
Externa à Instituição - ELIANA RODRIGUES - UNIFESP
Externa à Instituição - CAROLINE MARRONI CREMONEZ - UNIMAR
Externo à Instituição - JOAO MAURICIO PACHECO - USP
Externo à Instituição - ADRIANO GUIMARAES PARREIRA - UEMG