Banca de DEFESA: VIVIANA RODRIGUES CARDOSO

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : VIVIANA RODRIGUES CARDOSO
DATE: 01/07/2021
TIME: 13:30
LOCAL: Plataforma Google Meet
TITLE:

Callus induction and in vitro production of phenolic compounds in Bauhinia variegata L. (FABACEAE)

KEY WORDS:

Medicinal plant, callogenesis, secondary metabolites in vitro, cow's leg

PAGES: 62
BIG AREA: Multidisciplinar
AREA: Biotecnologia
SUMMARY:

Species of the genus Bauhinia are used in different parts of the world to treat various diseases. The species Bauhinia variegata L., known as “pata-de-vaca”, is a woody species of Cerrado, whose leaves are used in traditional medicine to treat infections and diabetes. Phytochemical studies indicate the presence of flavone; teaflavine; catechin; anthraquinone; flavone; flavanone; flavonol in the calluses of B. variegata The culture of plant tissues and, more specifically, the culture of calluses is a biotechnological tool that makes it possible to obtain bioactive substances of interest in vitro, through the optimization of cultures. The objective of this work was to establish an effective protocol for callus induction in leaf segments of Bauhinia variegata and to evaluate the phenolic substances present in the calluses of this species. For callogenesis, leaf explants from seedlings grown in vitro were inoculated in MS medium supplemented with 30 g L-1 of sucrose, solidified with 7 g L-1 of agar and added with 2,4-D (0; 4,52 ; 9.05; 18.10 μM) and BAP (0.4.44; 8.88; 17.75 μM) and their possible combinations, in the presence and absence of light. After 45 days of inoculation, the percentage of callus induction, fresh and dry matter and the contents of total phenols and flavonoids were evaluated, using usual methodologies. Calluses induced in media with 4.52 µM 2,4-D + 4.44 µM BAP T7 (compact calluses), in the absence of light. The growth curve was established from calluses in the presence of light. For the phytochemical study, the crude hydroethanolic extract (EHEB) was obtained by percolation, which was subjected to the liquid-liquid partition of ethyl and butanol. The isolation and identification of substances present in the ethyl acetate fraction were performed using the techniques of High Performance Liquid Chromatography coupled to DAD detectors and spectrometer. The results showed that calluses induced in medium supplemented with 4.52 µM 2.4-D + 4.44 µM BAP, in the presence of light, presented a good percentage of induction, considerable values of fresh and dry matter and, mainly, the highest compound contents phenolic.

BANKING MEMBERS:
Presidente - 395916 - ANA HORTENCIA FONSECA CASTRO
Interna - 1540495 - LUCIANA ALVES RODRIGUES DOS SANTOS LIMA
Externo à Instituição - MAIRON CESAR COIMBRA
Externa à Instituição - SUZAN KELLY VILELA BERTOLUCCI