leishamiasis; parasitic diseases; chalcones; toxicity tests.

Leishmaniasis are zoonoses that can affect skin, mucous membranes or viscera, affecting humans and / or dogs. They are caused by flagellated protozoa of the Trypanosomatidae family, of the genus Leishmania, with more than 20 species worldwide and are transmitted by sand flies, of the genus Lutzomya. Chalcones are precursor chemicals for the biosynthesis of flavonoids, found abundantly in plants. In addition to their antiparasitic action, they have anti-inflammatory, anti-tumor, analgesic, antiviral and antimicrobial activities. This study aimed to evaluate the antileishmania activity of synthetic hybrid chalcones in the promastigote and amastigote forms of Leishmania (Leishmania) amazonensis, in addition to evaluating the cytotoxicity of the compounds in murine models. For this purpose, in vitro cultures of the strains were performed using Liver Infusion Triptose (LIT) and Roswell Park Memorial Institute (RPMI), supplemented with fetal bovine serum and streptomycin, and drug candidates were subsequently tested using the MTT colorimetric assay. . In animal models (mice) cytotoxicity tests were also performed using cells obtained from the brain, liver and spleen. The partial results showed that the two compounds tested showed IC 50 compatible with the control drug, in addition to presenting low toxicity.