Banca de DEFESA: KEIZA PRISCILA ENES DOS SANTOS

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : KEIZA PRISCILA ENES DOS SANTOS
DATE: 30/09/2021
TIME: 13:30
LOCAL: banca por videoconferencia
TITLE:

Abamectin promotes behavior changes and liver injury in zebrafish

KEY WORDS:

Avermectins, Abamectin, Toxicity, Liver, Behavior 

PAGES: 71
BIG AREA: Multidisciplinar
AREA: Biotecnologia
SUMMARY:

The indiscriminate use of pesticides is a worldwide concern due the environment contamination since it can cause deleterious effects to non-target organisms including the fishes. To analyze the effects of abamectin, a pesticide of the avermectin family, adult zebrafish (Danio rerio) were exposed to a commercial formula commonly used in Brazil. The animals were submitted to an acute (96 h) and to a short-term chronic exposure (15 days) of distinct concentrations of abamectin. The LC50 was determined and a histological study followed by an immunohistochemistry analysis for P-gp and HSP70 identification were performed on livers of the animals submitted to the acute and chronic treatment, respectively. Moreover, behavior patterns were observed daily in both trials. A LC50 value of 105.68 mg/L was determined. The histological analysis revealed a morphological alteration of the hepatocytes, glycogen accumulation, degeneration, and disorganization of the cytoplasm, and a pyknotic, irregular, and laterally located nuclei. The immunohistochemistry for HSP70 and P-gp showed strong staining in the hepatocytes of the control groups and progressive decrease as the concentration of abamectin increased. Changes were observed in body posture, movement around the aquarium, opercular activity, body color and search for food in the groups treated with abamectin. The results presented suggest that abamectin can affect the behavioral pattern of the animals, promote morphological changes, and decrease the expression of HSP70 and P-gp in zebrafish liver.

BANKING MEMBERS:
Presidente - 2875448 - RALPH GRUPPI THOME
Interno - 1689019 - RAFAEL CESAR RUSSO CHAGAS
Externo ao Programa - 1716137 - HELIO BATISTA DOS SANTOS
Externa à Instituição - CAMILA FERREIRA SALES - UFMG