Banca de QUALIFICAÇÃO: LUISA FERREIRA DA CRUZ

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : LUISA FERREIRA DA CRUZ
DATE: 18/02/2022
TIME: 14:00
LOCAL: google meet
TITLE:

Prospecting for lactic acid bacteria for use as a probiotic and evaluating the effect on vulvovaginal candidiasis and anxiety and depression

KEY WORDS:

Prospecting, probiotic, lactic acid bacteria, Leuconostoc mensenteroides, Schleiferilactobacillus harbinensis, vulvovaginal candidiasis, antifungal.

PAGES: 130
BIG AREA: Multidisciplinar
AREA: Biotecnologia
SUMMARY:

Probiotic bacteria are known for their ability to bring benefits to the host, such as maintaining the immune system, inhibiting the growth of pathogenic microorganisms and maintaining emotional well-being, and therefore, there is a growing interest in new lactic acid bacteria with probiotic potential. The aim of this study was to track probiotic lactic acid bacteria from different sources and verify the potential of Leuconostoc mensenteroides (M13), Schleiferilactobacillus harbinensis (Ca12), isolated by our research group and a mix of them (Ca12+M13), against an animal model of Vaginal infection caused by Candida albicans. In vitro probiotic tests included survival under low pH and bile salts, cell surface hydrophobicity, autoaggregation, coaggregation, antibacterial activity, and safety properties were evaluated based on the antibiotic resistance profile. In the experimental animal model of vaginal infection by Candida albicans, the administration of probiotics was performed through two approaches: vaginal probiotic administration to evaluate the therapeutic activity, and oral probiotic administration to evaluate the preventive activity. As a result of the prospection, 46 bacteria with characteristics of lactic acid bacteria were isolated, being 32 discriminated as genetically different through the RAPD technique, being these isolated from the sources, kefir (n=9), cheese (n=4) kombucha (n= 2), silage (n=3) and newborn feces (n=14). These were considered for probiotic potential tests, with most strains resistant to simulated gastric acidity and bile salts. High hydrophobicity was observed for four strains. Autoaggregation ranged from 9.31-62.24% and coaggregation ability demonstrated a wide range of variation in coaggregative phenotypes. In addition, the strains also showed varying levels of resistance to clinically important antibiotics. In the experimental animal model, both probiotic administration protocols were able to reduce the number of viable C. albicans recovered from vaginal washes and the number of leukocytes induced by the infection. However, the M13 strain showed greater efficacy through the therapeutic vaginal administration, and the Ca12 strain through the preventive oral administration, with no potentiation of the effects through the Mix of strains. Overall, the evaluation of the probiotic properties of a large set of lactic acid bacteria isolates was instrumental in analyzing the phenotypic characteristics of the isolates and gaining insights into personalized applications. prevention of vulvovaginal candidiasis respectively.

BANKING MEMBERS:
Interno - 1757978 - DANIEL BONOTO GONCALVES
Interno - 1675931 - PAULO AFONSO GRANJEIRO
Externa ao Programa - 1719911 - JULIANA TEIXEIRA DE MAGALHAES
Externa ao Programa - 1945166 - MAIRA DE CASTRO LIMA
Externo à Instituição - ANDRE LUIS MORAIS RUELA