Banca de QUALIFICAÇÃO: PABLO FELIPE RODRIGUES GONCALVES

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : PABLO FELIPE RODRIGUES GONCALVES
DATE: 13/05/2022
TIME: 08:00
LOCAL: https://meet.google.com/djo-awjr-avc
TITLE:

OPTIMIZATION OF BACTERIAL PRODUCTION OF LACTIC ACID IN WHEY SUPPLEMENTED WITH SOY EXTRACT AND ITS MICROENCAPSULATION FOR USE IN FRUIT ICE CREAM

KEY WORDS:

Biotechnology, probiotics, optimization, encapsulation, lactic acid bacteria.

PAGES: 64
BIG AREA: Multidisciplinar
AREA: Biotecnologia
SUMMARY:

Probiotics are live microorganisms used as food supplements with beneficial effects related to the improvement of intestinal balance. Among the microorganisms considered probiotics, only those classified as lactic acid bacteria (LAB) are considered important in relation to food. The high cost of production makes processes with probiotics unfeasible, so the use of renewable sources such as whey and low-cost supplementation can make the industrial production of these bacteria possible. This work aimed to optimize the production of biomass and microencapsulation of the Can7 strain, for use as a probiotic in fruit sorbet. By supplementing the whey with soy flour, the fractional factorial was performed in the Minitab18 program. High biomass concentrations were achieved using soybean flour extract as a medium supplementation, maintaining savings of 98.86% and 98.65% in relation to the use of peptone and yeast extract. The encapsulation of the Can7 strain by the emulsification/internal gelation technique with sodium alginate (5%) showed an efficiency of 87.5%. The increase in the survival rate under gastro conditions of the microcapsule was 65.62% in relation to the free cell. In the intestinal environment test, cell survivals in microcapsules were 57.69% higher than free ones. The microcapsules do not change the sensory characteristic of the sorbet. We conclude that soybean meal extract is a great option for a nitrogen source without loss of biomass production of the Can7 strain, making it possible to scale up the bioprocess. The microcapsule was effective in strain protection and a good resource to save on the amount of cells needed in probiotic foods.

BANKING MEMBERS:
Presidente - 1675931 - PAULO AFONSO GRANJEIRO
Interno - 1757978 - DANIEL BONOTO GONCALVES
Externa ao Programa - 2045083 - TELMA PORCINA VILAS BOAS DIAS