Banca de QUALIFICAÇÃO: WELLINGTON FERNANDES DE CARVALHO

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : WELLINGTON FERNANDES DE CARVALHO
DATE: 19/09/2022
TIME: 09:00
LOCAL: Google Meeting
TITLE:

EVALUATION OF THE COCKTAIL EFFECT OF DIPIRONE, PARACETAMOL AND NIMESULIDE USING THE EMBRYONIC DEVELOPMENT OF ZEBRAFISH (Danio rerio) AS EXPERIMENTAL MODEL

KEY WORDS:

Environmental toxicology;emerging pollutants;embryotoxic assay;fish;environmental impact.

PAGES: 36
BIG AREA: Ciências Biológicas
AREA: Morfologia
SUBÁREA: Embriologia
SUMMARY:

Pharmaceutical products, especially medicines, are of great importance to society and their consumption has been increasing exponentially. Pharmaceutical substances are part of a new class of xenobiotics known as emerging pollutants and have become a major public health concern. Fish, especially the embryonic development of zebrafish, have been widely used in environmental assessment research. Most studies using fish have focused mainly on toxicological evaluation of isolated drugs, however, the combined effects (cocktail) of these xenobiotics have been little explored and this is the possible scenario found in the environment. It was described that the drugs most incorrectly discarded by drugstore users in the city of Divinópolis, MG were dipyrone, paracetamol and nimesulide. In this sense, our objective was to evaluate the cocktail effect of dipyrone, paracetamol and nimesulide during the embryonic development of zebrafish. First, the lethal concentration of 50 % in 96 h (CL 50-96h) was obtained for the drugs of interest. After the determination of LC50, the following experimental cocktail groups were developed: Control (E3 medium), LC 50/2, LC 50/5, LC 50/10 and LC 50/20. Embryos with 6-8 hours post fertilization (hpf) were submitted to cocktail treatment and the following times were evaluated: 24, 48, 72 and 96 h. In the morphometric evaluation of the embryos, the number of heartbeats, hatching rate and embryonic mortality rate by development time were obtained. After euthanasia, using the hypothermic shock technique, total length (from the nose to the end of the tail), size of the cardiac area and yolk sac edema were obtained. In addition, anatomical abnormalities (teratogenic effects) in the embryos were verified. After photomicrography, the specimens were fixed in Bouin's liquid for 2-4 h at room temperature and then preserved in 70º alcohol. The LC50/96h calculated for dipyrone, paracetamol and nimesulide were 4.69 mg/ml, 799.98 μg/ml and 0.92 μg/ml, respectively. After the toxicological tests, it was possible to observe that the embryos exposed to the cocktail a significant increase in the mortality rate and decrease in the hatching rate. In addition, teratogenic effects were observed in the embryos, such as malformation of the head, curvature of the tail and spine, yolk and pericardial edema and loss of pigmentation. Another result observed was the proportional relationship between the increase in concentrations and the intensification of the changes observed in the embryos. Finally, we believe that the present work, based on the results obtained, will possible understanding and extrapolate to nature the damage caused to living beings due to the accidental exposure of these xenobiotics released into the environment.

BANKING MEMBERS:
Presidente - 1716137 - HELIO BATISTA DOS SANTOS
Interno - 1300132 - WHOCELY VICTOR DE CASTRO
Interno - 1526269 - LEANDRO AUGUSTO DE OLIVEIRA BARBOSA
Externa à Instituição - CAMILA MARIANGELA PACHECO - UEMG