Banca de QUALIFICAÇÃO: JICAURY ROBERTA PEREIRA DA SILVA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : JICAURY ROBERTA PEREIRA DA SILVA
DATE: 29/11/2022
TIME: 09:00
LOCAL: Google Meeting
TITLE:

EVALUATION OF THE TOXICITY OF PLASTIFYERS USED AS DRUGS EXCIPIENTS, USING FISH AS AN EXPERIMENTAL MODEL

KEY WORDS:

Biomarkers; Dibutylphthalate; Diethylphthalate; embryotoxicity; Phthalates; zebrafish.

PAGES: 63
BIG AREA: Ciências da Saúde
AREA: Farmácia
SUBÁREA: Análise Toxicológica
SUMMARY:

The wide production and use of medicines by the population means that pharmaceutical products and their derivatives are being observed in different aquatic environments. Studies have detected that certain plastic additives used in the coating of pharmaceutical forms can harm the patient's health. Among plasticizers, phthalates, derived from phthalic acid (C₆H₄(COOH)₂), have been widely investigated for their toxicological potential. Phthalate esters (PAEs) are a group of synthetic chemicals with a broad spectrum of commercial applications and are among the most widespread pollutants in industrialized areas. However, PAEs have the ability to interact adversely in several biological processes, such as growth, metabolism, endocrine and immune systems. Thus, the present proposal aims to identify the toxicological effects of low molecular weight phthalates such as diethyl phthalate (DEP) and dibutyl phthalate (DBP) using the Fish Embryo Acute Toxicity (FET) technique whose animal model is the Danio rerio (zebrafish). For that, we were interested in the concentration for the lethal in 96 h (CL 96h) for the phthalates. After LC 50 survival for BPD, embryos were exposed to a concentration of 0.70mg/L and the following were presented: 24, 48, 72 and 96h identification of the toxic effects of dibutylphthalate. By time of evaluation, the rates of hatching and embryonic mortality were recorded; the number of times a few minutes per minute and 48h. The toxicological experiment was programmed in the semi-static system. After euthanasia, using the hypochlorite solution for 5 minutes, the measurement of the total length (from the snout to the end of the tail). In addition, anatomical abnormalities (teratogenic effects) in the embryos will be verified, such as: malformation of the head, spine and tail. From these data, we believe that the present project will present the possible toxic effects of these low molecular weight phthalates and their mixture during the initial development of D. rerio. In addition, we hope that the results obtained can also allow us to understand the nature of the damage caused and extra living due to accidents to these xenobiotics that are released into the environment.

BANKING MEMBERS:
Presidente - 1716137 - HELIO BATISTA DOS SANTOS
Interna - 1080217 - FARAH MARIA DRUMOND CHEQUER BALDONI
Externa à Instituição - CAMILA FERREIRA SALES - UFMG