Banca de DEFESA: JICAURY ROBERTA PEREIRA DA SILVA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : JICAURY ROBERTA PEREIRA DA SILVA
DATE: 16/02/2023
TIME: 13:30
LOCAL: Google Meeting
TITLE:

EVALUATION OF THE TOXICITY OF PLASTIFYERS USED AS DRUGS EXCIPIENTS, USING FISH AS AN EXPERIMENTAL MODEL


KEY WORDS:
Dibutylphthalate; Diethylphthalate; embryotoxicity; Phthalates; zebrafish.

PAGES: 68
BIG AREA: Ciências da Saúde
AREA: Farmácia
SUBÁREA: Análise Toxicológica
SUMMARY:
The widespread production and use of medicines by the population results in greater
observation of pharmaceutical products and their derivatives in aquatic environments.
Certain plastic additives used in the coating of dosage forms can cause harm to the
patient's health and the environment. Among plasticizers, phthalate esters (PAEs),
derived from phthalic acid (C6H4(COOH)2), have been widely investigated for their
toxicological potential. PAEs have the ability to adversely interact with various biological
processes, such as growth, metabolism, endocrine and immune systems. The present
study aims to identify the toxicological effects of low molecular weight phthalates such
as diethylphthalate (DEP) and dibutylphthalate (DBP) using the Fish Embryo Acute
Toxicity (FET) technique whose animal model is the Danio rerio (zebrafish). Embryos
were exposed to concentrations of 0.70- 0.35- 0.17- 0.08 mg/L of DBP and 24- 12- 6- 3
mg/L of DEP, and evaluated at times of 24, 48, 72 and 96 hours post fertilization (hpf)
for identification of toxic effects. The hatching rate and embryonic mortality were
evaluated, as well as the appearance and number of somites, the number of heartbeats per
minute and length, in addition to anatomical abnormalities (teratogenic effects) such as
malformation of the head, spine and tail. After exposure to PAEs, embryos exposed to
higher concentrations of DBP and DEP showed an increase in the number of somites,
irregular heartbeat and higher heart rate when compared to the control group. DBP more
frequently induced pericardial edema in the embryos, whereas DEP had a greater effect
on the hatching rate, and induced a higher rate of malformations in the tail, at the highest
concentration (24mg/L) 75% of the embryos showed necrosis or crooked tail. DBP is
more toxic than DEP as it induces adverse effects on embryos at considerably lower
concentrations. Phthalate contamination is a public health issue and needs to be given due
attention. In this way, we hope that the results obtained can enable the understanding and
extrapolate to nature the damage caused to living beings due to exposure to these
xenobiotics that are released into the environment.

BANKING MEMBERS:
Interno - 1300132 - WHOCELY VICTOR DE CASTRO
Interno - 1716137 - HELIO BATISTA DOS SANTOS
Externa ao Programa - 1006938 - LUCIANA XAVIER PEREIRA
Externa à Instituição - LUDMILA SILVA BRIGHENTI - UEMG
Externa à Instituição - CAMILA FERREIRA SALES - UFMG
Notícia cadastrada em: 17/02/2023 11:15
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