Banca de DEFESA: FELLIPE ALEXANDRE ALVES MORAES

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : FELLIPE ALEXANDRE ALVES MORAES
DATE: 27/10/2023
TIME: 14:00
LOCAL: https://meet.google.com/ypa-iuqw-cmv
TITLE:

ASARALDEHYDE: EVALUATION OF THE ANTI-INFLAMMATORY, ANTI-NOCICEPTIVE ACTIVITY, ELUCIDATION OF THE PROBABLE MECHANISMS OF ACTION AND TOXICITY OF A BIOACTIVE METABOLITE FROM Duguetia furfuracea

KEY WORDS:

Anti-inflammatory, Antinociception, Toxicity, Duguetia furfuracea, Asaraldehyde.

PAGES: 101
BIG AREA: Ciências da Saúde
AREA: Farmácia
SUMMARY:

About 30% of the world's adult population suffers from chronic pain. Among the main causes of chronic pain, the pathologies of inflammatory etiology stand out. Besides the drugs traditionally used not achieving the desired therapeutic outcome, they present a significant number of side effects that hinder the success of the treatment. Finding new pharmacological alternatives for the treatment of pain must be a priority for the scientific community. Ethnopharmacology has proven to be an important tool in the search for new pharmacological alternatives, by researching the traditional uses of natural products and their derivatives. This study had as its main objectives to evaluate the anti-inflammatory and antinociceptive activity, neuromotor effects, toxicity, as well as the probable mechanisms of anti-inflammatory and antinociceptive action through an in vivo model of asaraldehyde, a bioactive metabolite from Duguetia furfuracea (A. St.-Hil.) Benth. & Hook. f. To this end, we used the carrageenan-induced paw edema model to investigate the anti-inflammatory properties, the acetic acid-induced writhing, formalin, and hot plate tests to evaluate the antinociceptive properties, the rotating bar test (rotarod) to assess the neuromotor impact of asaraldehyde, and in silico ADMET (Absorption, Distribution, Metabolism, Excretion, and Toxicity) property evaluation software, along with the in vivo acute oral toxicity test, to determine the acute toxicity of asaraldehyde. The project was submitted to and approved by the UFSJ IACC. In the paw edema test, asaraldehyde showed antiedematogenic potential, reducing edema progression and inhibiting polymorphonuclear leukocyte migration. The induced abdominal contortions test signaled a reduction of the nociceptive response, the same could be observed in the formalin test in both phases. The hot plate test confirmed the central activity of asaraldehyde in all dosages. Any neuromotor changes from oral administration were ruled out in the rotarod test. When the effects of asaraldehyde associated with antagonists were evaluated in the formalin test, the participation of the opioid system in the mechanisms of antinociception was evidenced. The results confirm the information obtained in the literature about structural analogues of asaraldehyde (alpha-asarone, 2,4,5-trimethoxystyrene). In-silico toxicity evaluation, as well as acute oral toxicity tests, demonstrated that asaraldehyde has an adequate safety profile for prototype compounds with pharmacological potential, with an indication of hepatic changes only at the highest dose. Additional studies to evaluate pharmacokinetics, toxicity under prolonged exposure, as well as reproductive, mutagenicity, and carcinogenicity studies should be performed to confirm the findings described about its toxicity.

BANKING MEMBERS:
Interna - 1741307 - ADRIANA CRISTINA SOARES DE SOUZA
Externo à Instituição - ELIAS BORGES DO NASCIMENTO JUNIOR
Interna - 1080217 - FARAH MARIA DRUMOND CHEQUER BALDONI