Banca de DEFESA: DAYANA FERREIRA FERNANDES

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : DAYANA FERREIRA FERNANDES
DATE: 26/03/2024
TIME: 13:30
LOCAL: Google Meeting
TITLE:

TOXICOLOGICAL EVALUATION OF THE DELETORY EFFECTS OF THE BENZODIAZEPINES: CLONAZEPAM AND ALPRAZOLAM ON THE INITIAL DEVELOPMENT OF ZEBRAFISH (Danio rerio)

KEY WORDS:

Teratogenesis, Anxiolytics, Environmental impact, Embryogenesis; toxicity

PAGES: 86
BIG AREA: Ciências da Saúde
AREA: Farmácia
SUBÁREA: Análise Toxicológica
SUMMARY:

Introduction: Pharmaceutical products have been observed in the environment, and environmental contamination by these emerging pollutants can occur in several ways, which can consequently cause serious ecological consequences for non-target organisms and even human health. Objectives: To evaluate the toxicological effects individually and as a cocktail of benzodiazepines (BZDs), clonazepam (CLO) and alprazolam (ALP) during the early development of zebrafish (Danio rerio). Material and methods: To evaluate the toxicological effects of BZDs during early embryonic development of zebrafish, Fish Embryo Toxicity (FET) was used. The lethal concentrations that promote 50% of the death of zebrafish embryos (LC50) were calculated, the fish were subjected to the following concentrations: ALP (0.125; 0.25; 0.50; 1.0 and 3.0 mg/ L) and CLO (20; 50; 100; 120 and 150 mg/L). After determining the LC50, an embryotoxic assay was carried out using concentrations of ALP and CLO alone or in a cocktail, to evaluate the lethal (mortality) and sublethal effects (heart beat per minute (bpm), hypoactivity, total length and morphological abnormalities). Results: An LC50 of 2 mg/L for ALP and 95 mg/L for CLO were found for zebrafish embryos exposed to increasing concentrations of BZDs during FET. In the embryotoxic assay with BZDs, a delay in hatching of embryos with the cocktail at higher concentrations was observed compared to the control group, as well as greater mortality after hatching. Embryos exposed to concentrations of ALP and CLO showed significant mortality results (p < 0.05) when compared to the control group. Toxicity was greater with increasing drug concentration in individual experiments, especially in mixtures. The embryos subjected to BZDs, in isolation and in the cocktail, showed anatomical abnormalities such as pericardial and yolk sac edema and spinal and tail malformations and depigmentation. Conclusion: In this present work, it was possible to observe toxic effects arising from the exposure of BZDs, both individually and in the form of an exposure cocktail during the initial development of D. rerio. Exposure to BZDs, especially at higher concentrations, promoted several morphological changes in zebrafish, and the appearance of anatomical malformations can directly affect their survival in the environment. Therefore, BZDs released inappropriately into the environment have a potential toxic effect on aquatic life and human health.

BANKING MEMBERS:
Interno - 1716137 - HELIO BATISTA DOS SANTOS
Interno - 1526269 - LEANDRO AUGUSTO DE OLIVEIRA BARBOSA
Externo ao Programa - 2875448 - RALPH GRUPPI THOME
Externa à Instituição - AMANDA LUISA DA FONSECA - UNA
Externa à Instituição - ANA GABRIELA SILVA OLIVEIRA - UFSJ
Externo à Instituição - ANDERSON KELVIN SARAIVA MACÊDO - UofC