Banca de DEFESA: ISABELLA FERREIRA SILVA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : ISABELLA FERREIRA SILVA
DATE: 01/10/2021
TIME: 13:30
LOCAL: virtual
TITLE:

Evaluation of the potential environmental risk of chloroquine diphosphate and hydroxychloroquine sulfate using the early development of Danio rerio as an experimental model.

KEY WORDS:

Teratogenesis, Environmental impact, Fish, Embryotoxicity, 4-aminoquinoline.

PAGES: 73
BIG AREA: Ciências da Saúde
AREA: Farmácia
SUMMARY:

Hydroxychloroquine sulfate (HCQ) and chloroquine diphosphate (CQ), despite their inefficacy, have been suggested as antiviral drugs in the fight against SARS-CoV2 in several countries, especially in Brazil.  In this sense, the increased consumption of these drugs for the treatment of COVID-19 may promote a potential environmental and public health risk. In this scenario, the objective was to evaluate the toxicity of sublethal concentrations of HCQ and CQ in Danio rerio fish embryos/larvae. The lethal concentration (LC50) of HCQ and CQ was calculated at 96 h. After obtaining the LC50 value, the toxicological assay was performed, in which embryos/larvae were subjected to sublethal concentrations of HCQ and CQ. Hatching rates, heartbeat frequency, and deformities in embryos/larvae exposed to the drugs were evaluated. The LC50 for HCQ was 560 µM and for CQ was 800 µM. Fish embryo acute toxicity (FET) assays were performed up to 96 h, in the semi-static system for HCQ and CQ. Thus, embryos/larvae exposed to sublethal concentrations of HCQ and CQ showed delayed hatching at 72 h and increased heart rate, edema in the pericardium and yolk sac area, and teratogenic effects such as crooked spine and shorter than normal tail at 96 h. The results show that HCQ and CQ are toxic to fish, especially in early development and understanding the mechanisms of toxicity, allowing us to extrapolate the adverse effects of these drugs when they reach the aquatic environment in the pandemic setting of COVID-19.

BANKING MEMBERS:
Externa à Instituição - CAMILA FERREIRA SALES - UFMG
Interna - 1080217 - FARAH MARIA DRUMOND CHEQUER BALDONI
Externo ao Programa - 1422156 - FERNANDO DE PILLA VAROTTI
Presidente - 1716137 - HELIO BATISTA DOS SANTOS
Externo à Instituição - KINULPE HONORATO SAMPAIO - UFVJM
Externo ao Programa - 2875448 - RALPH GRUPPI THOME