Banca de DEFESA: LILIANE DIAS CAMPOS

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : LILIANE DIAS CAMPOS
DATE: 15/12/2021
TIME: 08:00
LOCAL: virtual
TITLE:

Development of an Analytical Method for The Determination of Lithium and a Study for Monitoring SUS Patients with Clinical Picture of Bipolar Disorder

 

KEY WORDS:

Bipolar Mood Disorder, lithium, validation method, UV-VIS spectrophotometry, thorin.

 

PAGES: 82
BIG AREA: Ciências da Saúde
AREA: Farmácia
SUMMARY:

Introduction: Bipolar affective disorder is a chronic multicomponent disease that involves episodes of severe mood disorders, neuropsychological deficits, immunological and physiological changes. Lithium carbonate is a more suitable mood stabilizer drug, but the one that can be more toxic. In search of an alternative to this impasse, analytical methods to measure the serum amount in patients would be ideal. Many techniques are already used today, but they are very costly. The UV-VIS methodology would be a simpler and more accessible option.

Objectives: To develop an analytical method for the determination of serum lithium using UV-VIS spectrophotometry in order to enable a study for further therapeutic monitoring of patients treated by the Unified Health System (SUS) in Divinopolis, MG.

Methods: The study was divided into 2 steps: 1) to develop and validate an analytical method for the determination of serum lithium in blood samples. Different complexants were tested at a concentration of 7.25 x10-3 mol L-1. The experiments were carried out using the thorin complexant, which presented a better analytical condition. The method was validated following the Eurachen and Inmetro guidelines. 2) to propose a study model to monitor serum lithium in patients with bipolar disorder, who are assisted by the SUS, residing in Divinópolis, MG.

Results: Through a univariate optimization, a viable spectrophotometric method was developed to determine lithium in blood samples. The complexing agent used was Thorin 25% m v-1 and the linear working range was 0.2 to 2.0 μg/mL, which exhibited good precision, accuracy and sufficient sensitivity to allow the quantification of Lithium in patient samples. Eighty-five samples of patients using the drug lithium carbonate were analyzed by the method, and of these, only 6 were outside the therapeutic range, which showed that patients assisted in the SUS in Divinopolis have their serum dosages, relatively within the therapeutic drug range.

BANKING MEMBERS:
Externa à Instituição - SILVÉRIA NEVES DE PAULA E SOUZA - IFMG
Presidente - 1849106 - FRANK PEREIRA DE ANDRADE
Interno - 1150851 - RENE OLIVEIRA DO COUTO