Banca de DEFESA: CONCEIÇÃO APARECIDA LISBOA COELHO

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : CONCEIÇÃO APARECIDA LISBOA COELHO
DATE: 08/12/2021
TIME: 14:00
LOCAL: virtual
TITLE:

Development of an Analytical Method for The Determination of Lithium and a Study for Monitoring SUS Patients with Clinical Picture of Bipolar Disorder

 

KEY WORDS:

Hemodialysis, aluminum, UV–VIS Spectrophotometric Determination

PAGES: 73
BIG AREA: Ciências da Saúde
AREA: Farmácia
SUMMARY:

Aluminum ion is considered toxic for patients with chronic renal failure, especially for patients on hemodialysis due to exposure to the large volume of water used in preparing the dialysate. The Al³⁺ cation present in the dialysate can cross the dialyzer membrane into the bloodstream, and through transferrin be transported to various organs, mainly to the bones, lungs and brain, further weakening the renal patient's health. Currently, little is said about Al³⁺ intoxication problems in hemodialysis patients. The implantation of a water treatment system with reverse osmosis contributed a lot to avoid this type of problem, but the poisoning continues in a silent way through the accumulation of aluminum, mainly in the bones. One of the ways to increase safety is by monitoring Al³⁺ in the water used. Thus, the purpose of this work was to develop a technique by UV-VIS spectrophotometry, in order to quantify aluminum in water for hemodialysis, considering that this technique is not costly and can be easily performed. The method developed used the chromogenic reagent alizarin, 1,2-dihydroxy-9,10-anthraquinone – (DHA) to complex Al³⁺. A factorial design with central composite 2³ was applied to obtain the best pre-concentration conditions by cloud point, optimized in an ultrasonic bath. The optimal reaction conditions were experimentally defined at pH 5,59 with biphthalate/KCl buffer, Al³⁺-DHA molar ratio of 1:4.1, cationic surfactant, benzalkonium chloride 9,4 µL  at 5 % m v^–1 and sonication for 5 minutes. The sample was pre-concentrated by cloud point using 0.47% anionic surfactant (triton X–114). This process took place at a temperature of 60ºC on a heating plate. The absorbance reading was performed in a UV–VIS spectrophotometer at 520 nm, using 1 mL of the pre-concentrated sample and 5 drops of methanol. The linear range obtained was from 0.04 to 0.1 µg mL^–1. The method presented adequate precision with CV less than 5% and adequate accuracy, with recovery in the range of 94.6 to 105.9%. The limits of detection and quantification were set at 0.006 and 0.02 µg mL^–1 respectively.

 

BANKING MEMBERS:
Externa à Instituição - VANESSA MOREIRA OSÓRIO - UFES
Presidente - 1849106 - FRANK PEREIRA DE ANDRADE
Externo ao Programa - 1689019 - RAFAEL CESAR RUSSO CHAGAS