Banca de QUALIFICAÇÃO: TUANIA NATACHA LOPES SILVA

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : TUANIA NATACHA LOPES SILVA
DATE: 27/05/2022
TIME: 08:30
LOCAL: https://meet.google.com/fig-ugdh-hfz
TITLE:

Biochemical study of biomass production by lactic acid bacteria isolates and its application in the cosmetics industry

KEY WORDS:

lactic acid bacteria, probiotics, lysis, inactivation, antimicrobial, cosmetic

PAGES: 30
BIG AREA: Ciências Biológicas
AREA: Bioquímica
SUMMARY:

Disorders in the skin microbiota are related to infections and inflammation causing diseases such as acne and others. Alternatives for treatment are probiotic strains. Probiotics are live microorganisms that, when administered in adequate amounts, confer a health benefit on the host. The application of lysates and inactivates in the cosmetic industry is a trend. As long as the beneficial effects are maintained, they have advantages such as transport, shelf life, risk of translocation and consumer infection. The aim of this study was to evaluate the antimicrobial activity of cell-free, lysed and inactivated supernatant, viability, sublethal damage, influence of the culture medium in different methods, and encapsulation of LAB isolate with cosmetic application. Cell-free supernatants from the fifteen LAB strains did not inhibit the growth of Corynebacterium xerosys ATCC 373 and Pseudomonas aeruginosa ATCC 15442. The only methods capable to inactivating all strains were the thermal methods. The viability and sublethal damage was dependent on the strain, culture medium and lysis or inactivation method. The lysis and inactivation methods did not maintain antimicrobial activity against Cutibacterium acnes ATCC 6919. The SEM of the strains in whey showed organic matter involving the cells, which was not observed in MRS. The emulsification/internal gelation technique with 5% sodium alginate provided the encapsulation of 61.94% without lyophilization and 37.83% with lyophilization, without gastrointestinal survival. Physicochemical characteristics were analyzed by FTIR and optical microscope. The microcapsule lost anti-acne activity. We conclude that LAB in its lysed or inactivated and encapsulated forms did not maintain promising anti-acne activity.

BANKING MEMBERS:
Presidente - 1675931 - PAULO AFONSO GRANJEIRO
Externa ao Programa - 2045083 - TELMA PORCINA VILAS BOAS DIAS
Externa à Instituição - MARIA ESPERANZA CORTES SEGURA