Banca de DEFESA: MARIA AUXILIADORA DE OLIVEIRA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : MARIA AUXILIADORA DE OLIVEIRA
DATE: 26/08/2022
TIME: 14:00
LOCAL: https://meet.google.com/nfr-tfeg-ibj
TITLE:

Evaluation of the potential application in the environment and health of a biosurfactant produced from microorganisms

KEY WORDS:

biosurfactants, applications, lipopeptides, environment

PAGES: 81
BIG AREA: Ciências Biológicas
AREA: Bioquímica
SUMMARY:

Biosurfactants are produced by various microorganisms. The biotechnological properties of biosurfactants (BS) are widely described in the literature. Thus, this work aimed to evaluate the potential of application of the lipopeptide, produced from a microorganism, in face of the bactericidal, fungicidal, effluent decanting properties, in the spreading of motor oil, in the bioremediation of motor oil in the sand, in the bioremediation of heavy metals in soil and plants, in addition to its toxicity in in vivo assays with Artemia salina. As a result, it was demonstrated that the lipid at a concentration of 500 mg/mL presented an inhibition halo of 9 mm and an MIC value of 0.08 mg/mL for the Gran-negative bacterium Aeromonas hidroplyla, while for the filamentous fungus Colletotrichum sp. no inhibitory activity was detected. The qualitative evaluation of the lipopeptide in the treatment of effluents showed the ability to decant the sewage effluent in 24 h, while the one with aluminum sulfate took 30 min. Visual analysis showed that the lipopeptide performed better in the oil dispersion area when compared to Triton X-100 and SDS. The oil scattering ratio was 75, 48.7 and 59.7% for BS, Triton X-100 and SDS, respectively. After 24 h, it was possible to demonstrate that the oil in the presence of BS was concentrated in the center of the plate. Visual analysis of the bioremediation of motor oil in sand showed that the use of lipopeptide at a concentration of 0.1 mg/mL was more efficient than at a concentration of 0.5 mg/mL and in relation to the use of 0.1 mg/mL of SDS. The lipopeptide in the concentration of 0.1 and 0.5 mg/mL showed a recovery rate of motor oil from contaminated sand equivalent to 80 and 23%, respectively. SDS at a concentration of 0.1 mg/mL showed a recovery rate of 64%. At low concentrations (up to 0.1 mg/mL), the supernatants were non-toxic and did not affect swimming behavior or the survival rate of brine shrimp. At high concentrations (0.5 and 1 mg/mL), the mortality rate for the lipopeptide was 10%, while for the SDS compound it was 100%. We conclude that the lipopeptide produced from a microorganism showed a high potential for application in various sectors of the industry, such as the environment and health.

BANKING MEMBERS:
Presidente - 1675931 - PAULO AFONSO GRANJEIRO
Interno - 1757978 - DANIEL BONOTO GONCALVES
Externo à Instituição - ADRIANO GUIMARAES PARREIRA