Banca de DEFESA: ÍTALO LEONARDO DIOGO

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : ÍTALO LEONARDO DIOGO
DATE: 30/03/2023
TIME: 09:00
LOCAL: https://meet.google.com/mna-mukw-yrn?hs=122&authuser=1
TITLE:

BEHAVIORAL AND BIOCHEMICAL EVALUATION IN CORTEX AND HIPPOCAMPUS OF RATS FED A CAFETERIA DIET AND TREATED WITH ATORVASTATIN

KEY WORDS:

Obesity, Atorvastatin, Apoptosis, Neuroprotection, Anxiety and Memory.

PAGES: 51
BIG AREA: Ciências Biológicas
AREA: Bioquímica
SUBÁREA: Biologia Molecular
SUMMARY:

Obesity is a chronic disease characterized by excessive accumulation of body fat, which can generate metabolic disorder in all systems, including the nervous system, producing a process of inflammation and mitochondrial dysfunction, mainly by excessive production of Reactive Oxygen Species (ROS). These disorders generate neuronal death and behavioral changes. Atorvastatin is a drug frequently used for hypercholesterolemia, almost always associated with obesity. The objective of this project was to evaluate behavioral and biochemical parameters in the hippocampus and cortex of male wistar rats fed a cafeteria-type diet and treated with atorvastatin. Male Wistar rats were divided into four groups: commercial diet - saline (CoSal), commercial diet - atorvastatin (CoArt), cafeteria diet - saline (CafSal), and cafeteria diet - atorvastatin (CafArt). Atorvastatin was administered by gavage at a dose of 10 mg/kg/day and saline at a dose of 0.9%, and the cafeteria groups received water with 20% sucrose. The animals were weighed daily and underwent behavioral anxiety testing on day 23 and memory tests on day 24. On the 25th day, the animals were euthanized, blood was collected for cholesterol quantification by colorimetric method, and the body was dissected for removal and weighing of retroperitoneal and epididymal fat. In addition, the cortex and hippocampus were dissected and stored in -80ºC freezer for further quantification of caspases and AIF by Western Blotting and for quantification of lipoperoxidation by TBARS method. The circulating cholesterol was affected by diet and normalized by atorvastatin. Regarding weight, there was no change in total weight, but the weighing of fat showed that there was accumulation of adipose tissue in the cafeteria animals. The cafeteria diet caused hypomobility and spatial memory damage in the animals, and atorvastatin inhibited these effects. The diet did not cause problems in de novo memory or anxiety, but the use of atorvastatin without the diet caused problems in these parameters. The expression of apoptotic proteins (AIF and caspases 8 and 9) was increased by the cafeteria diet and returned to control with the use of atorvastatin.

BANKING MEMBERS:
Interna - 1106384 - LUCIANA ESTEFANI DRUMOND DE CARVALHO
Externa à Instituição - JACQUELINE ALVES LEITE - UFG
Externo à Instituição - HÉRCULES RIBEIRO LEITE - UFMG