Banca de QUALIFICAÇÃO: VINÍCIUS SOUZA TARABAL

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : VINÍCIUS SOUZA TARABAL
DATE: 24/05/2023
TIME: 14:00
LOCAL: https://meet.google.com/ygm-hpzi-bjj
TITLE:

Impact of the N-dodecyl,N-Methylpolyethyleneimine on the Surfaces to Prevent Biofilm Formation and Biofilm Disruption

KEY WORDS:

biofilm, DMPEI, surface

PAGES: 32
BIG AREA: Ciências Biológicas
AREA: Bioquímica
SUMMARY:

Biofilms are structures formed by planktonic bacteria that adhere to a surface. The microorganisms present near patient beds are those commonly found in the microbiota of a healthy person, suggesting that the main contamination vectors are professionals and other patients in the hospital. There are several ways to combat a biofilm, and coating surfaces to prevent bacteria adhesion is one of them. In this work, we coated surfaces, commonly found in hospital and animal husbandry environments, with DMPEI, such as stainless steel, polystyrene, and polypropylene, and evaluated their ability to prevent the formation of biofilms of Escherichia coli, Klebsiella oxytoca, and Acinetobacter baumannii or disrupt a mature biofilm. We achieved an E. coli anti-adhesion of 87.16% and 98.81% on stainless steel and polystyrene, respectively. Stainless steel coated with DMPEI showed an anti-adhesion of A. baumannii of 99.66%. We were unable to achieve an anti-adhesion of K. oxytoca biofilm on polypropylene and disrupt a mature biofilm on a mold wall. The anti-adhesion of K. oxytoca was 88.0% on stainless steel. DMPEI paint showed 93.96% of E. coli anti-adhesion. The wall painted with DMPEI paint did not show adhesion of microorganisms for 7 days. DMPEI coated stainless steel maintains 90% E. coli anti-adhesion for 56 weeks. Long-term assays in walls and new strategies to disrupt mature biofilms are needed.

BANKING MEMBERS:
Presidente - 1675931 - PAULO AFONSO GRANJEIRO
Externa à Instituição - MARIA ESPERANZA CORTES SEGURA
Externa à Instituição - MAYRA ALEXANDRA TÉLLEZ CORRAL