Banca de QUALIFICAÇÃO: SILVIA RAMOS SILVA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : SILVIA RAMOS SILVA
DATE: 20/11/2023
TIME: 14:00
LOCAL: plataforma online; Google meet; meet.google.com/xjd-tbnh-mnm
TITLE:

MEMBRANE AND OXIDATIVE PARAMETERS IN WISTAR, NEONATE BRAIN AND JUVENILE CEREBELLUM, IN AUTISM ANIMAL MODELS

KEY WORDS:

Melatonin, VPA, Oxidative Stress, Na,K-ATPase, Autism Spectrum Disorder

PAGES: 69
BIG AREA: Ciências Biológicas
AREA: Bioquímica
SUBÁREA: Biologia Molecular
SUMMARY:

Melatonin is a hormone provided by the mother to the fetus during development, acting as an antioxidant in crucial cellular processes of the nervous system. The use of Valproic Acid (VPA) during pregnancy serves as a model for studying Autism Spectrum Disorder (ASD). We assessed whether the absence of maternal melatonin during intrauterine development results in damages similar to autism in offspring, as observed in animals exposed to VPA. Wistar rats were divided into five groups: pinealectomized with and without melatonin supplementation, a surgical procedure without pineal gland removal (SHAM), VPA injection on the 13th day of gestation, and saline injection as a VPA control. Two days after birth and at the fourth week of life, the offspring were sacrificed, and the neonatal brain and young rat cerebellum were collected and analyzed for GSH content, H₂O₂ production, lipid peroxidation, and Na,K-ATPase activity and its isoforms. Our neonatal results showed gender differences in oxidative stress levels, with males exhibiting higher levels than females. In the cerebellum, we observed similar effects in the offspring of pinealectomized rats and those exposed to VPA, including increased oxidative stress, lipid peroxidation, and decreased GSH, as well as deficits in Na,K-ATPase activity. Exogenous melatonin supplementation restored normal levels of oxidative parameters and the total activity and isoform α1-Na,K-ATPase in the cerebellum. In summary, this study shows that oxidative stress may play a crucial role in the etiology of ASD and underscores the importance of maternal melatonin in preventing neurodevelopmental disorders in offspring. Additionally, it points to possible gender differences that will be investigated in future studies.

BANKING MEMBERS:
Interna - 1716801 - CRISTIANE QUEIXA TILELLI
Externo à Instituição - FERNANDA GASPAR DO AMARAL - UNIFESP
Externo à Instituição - VICTOR RODRIGUES SANTOS - UFMG