Banca de DEFESA: WANESSA ALVES ARAUJO

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : WANESSA ALVES ARAUJO
DATE: 24/02/2021
TIME: 14:00
LOCAL: meet.google.com/emn-ksuv-qux
TITLE:

In vitro evaluation of the effect of Diazepam on ATPase activity, lipid content and oxidative stress 

of erythrocyte membranes

KEY WORDS:

Diazepam; Erythrocytes; Na,K-ATPase; Lipids; Oxidative stress.

PAGES: 72
BIG AREA: Ciências Biológicas
AREA: Bioquímica
SUMMARY:

Diazepam (DZP) is one of the most well-known benzodiazepines (BZDs), being widely used as an anxiolytic, anticonvulsant and muscle relaxant. Considering the increasing and indiscriminate consumption of anxiolytic drugs worldwide, together with the scarcity of studies that evaluate the effects of BZDs on blood cells, in addition to the fact that, after administration, DZP remains temporarily in the current blood, the aim of the present study was to evaluate the effect of DZP on the Na,K-ATPase and PMCA activities, plasma membrane integrity and parameters related to oxidative stress in human erythrocytes. For this, human blood was incubated with DZP 15 μg/mL (injectable or pure) for 30 and 60 minutes, at room temperature. Subsequently, the following steps were performed: preparation of erythrocyte membrane (ghost), lipid extraction, determination of osmotic fragility, dosage of total phospholipids and cholesterol and evaluation of the activity of antioxidant enzymes and oxidative parameters. Treatment with injectable DZP caused a decrease in the Na, K-ATPase activity, with no significant changes in the other parameters. The group treated with pure DZP showed a greater osmotic fragility when compared to the control group. A 43% increase in membrane phospholipid content was observed after 60 minutes of treatment. The lipid peroxidation index was not altered, but a decrease in H₂O₂ levels and in CAT and AChE activities was observed. In addition, other oxidative stress markers, such as GPx and GSH, were modulated by DZP in a time-dependent manner, and a 46% decrease in Na, K-ATPase activity was observed after 30 minutes of treatment. These results indicate that DZP was able to alter important biochemical parameters in erythrocytes, such as osmotic fragility, lipid content, oxidative stress and ATPasic activity of the membrane in a time-dependent manner. Thus, the several effects observed here reinforce the importance of studies that evaluate the effect of BZDs on erythrocytes, motivating the conscious and controlled use of these drugs and the search for alternative treatments, since the prolonged use of DZP by individuals who are already weakened by diseases that compromise blood cells can significantly affect them.

BANKING MEMBERS:
Presidente - 1692875 - VALERIA ERNESTANIA CHAVES
Externo ao Programa - 1039964 - ANDRE DE OLIVEIRA BALDONI
Externo à Instituição - JÚLIO ALBERTO MIGNACO - UFRJ