Banca de DEFESA: FELIPE FERREIRA SILVA

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : FELIPE FERREIRA SILVA
DATE: 22/02/2024
TIME: 14:00
LOCAL: https://meet.google.com/jyf-fqxw-waz
TITLE:

 Development of a CRISPR/Cas9 system in the filamentous fungi Penicillium chrysogenum for the expression of transcription factors XlnR and AraR and (hemi-)cellulase overproduction.

KEY WORDS:

CRISPR/Cas; Filamentous fungi; Transcription factors; (Hemi-)cellulases.

PAGES: 60
BIG AREA: Ciências Biológicas
AREA: Bioquímica
SUMMARY:

The increasing demand to produce renewable fuels (biofuels) to replace the use of fossil fuels is reflected in the global political scenario, and Brazil has a leading role in the participation of renewables in its energy matrix. Hemicellulose, the second most abundant fraction of plant biomass after cellulose, which until recently was treated only as waste, has been seen as a potential source to produce biofuels and other value-added materials. It is a heterogeneous polysaccharide complex, composed mainly of xylose and arabinose whose degradation requires the production of many different enzymes, classified as (hemi)-cellulases, which are induced by biopolymers or their derivatives and mainly regulated at the transcriptional level through transcription factors (TFs). Within these TFs, it is possible to highlight two, mainly produced by filamentous fungi. XlnR, the main regulator of this enzyme complex, and AraR, a secondary homologous factor and important regulator in joint action with XlnR. Filamentous fungi, organisms that naturally produce enzymes that degrade these polysaccharides, have very effective and biotechnologically attractive protein production and secretion systems. In this context, the present work aims to develop a gene editing system via CRISPR/Cas9 in the filamentous fungus Penicillium chrysogenum to overexpress the TFs XlnR and AraR and evaluate the influence of this overexpression on the production of (hemi-)cellulases.

BANKING MEMBERS:
Presidente - 1757978 - DANIEL BONOTO GONCALVES
Externo à Instituição - WAGNER RODRIGO DE SOUZA - UFABC
Externo à Instituição - LUCIANO GOMES FIETTO
Externo à Instituição - JONATAS OLIVEIRA DA SILVA
Externo à Instituição - JOÃO BATISTA RIBEIRO - EMBRAPA