Banca de DEFESA: CRISTIANE DE OLIVEIRA RENO

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
DISCENTE : CRISTIANE DE OLIVEIRA RENO
DATA : 11/08/2020
HORA: 14:00
LOCAL: Campus Centro-Oeste
TÍTULO:


BIOCHEMICAL EVALUATION OF THE EFFECT OF HYDROXYUREA IN ERYTHROCYTES TREATED IN VITRO.


PALAVRAS-CHAVES:
Hydroxyurea, erythrocyte, energy metabolism, antioxidant system,
reactive oxygen species and membranes.

PÁGINAS: 125
GRANDE ÁREA: Ciências Biológicas
ÁREA: Bioquímica
RESUMO:
Hydroxyurea (HU) is a low cost, easily administered orally and widely used in diseases
such as sickle cell anemia (SCA) and different types of cancer. The effects of HU on
the erythrocyte are not yet fully understood. In our work we evaluated the effects of HU
on the oxidative stress indicator profile, the antioxidant system enzyme activity, the
energy metabolism and the lipid profile of the erythrocyte membranes of healthy
individuals treated in vitro. Comparisons between the groups (control and treated with
0.6, 0.8 and 1.5mM HU) were made at the treatment time of 30 minutes and 1 hour. At
the 30 min treatment time there was a significant increase in almost all parameters
analyzed between the HU-treated blood groups and the control group with the
exception of pyruvate kinase (PK) enzyme activity. The 1h treatment showed a
significant reduction, in the 0.8mM HU concentration when compared to the control
group, in markers such as lipid peroxidation, Fe3+ and activity of some enzymes such
as glutathione reductase (GR), glucose-6-phosphate dehydrogenase (G6PD) and PK.
At the same time of treatment, the 0.8mM concentration, when compared to the 0.6mM
HU concentration, significantly reduced the H2O2 content, lipid peroxidation, reduced
glutathione (GSH) and the activity of enzymes such as: hexokinase, G6PD and
superoxide dismutase (SOD). HU may be preferentially stimulating the activation of
the pentose phosphate pathway rather than the glycolytic pathway in erythrocytes, in
addition the concentration of 0.8mM seems to be more efficient with regard to
protection against free radicals and could be preferentially adopted in clinical practice.
diseases such as SCA, where oxidative stress is already in place. In addition, a direct
HU treatment on erythrocyte membranes (GHOST) was performed within 20 minutes
and the Na+ ,K+-ATPase activity and membrane lipid content were evaluated. HU
induced increased Na+ ,K+-ATPase and cholesterol activity in the treated groups and
was able to provide erythrocyte membrane modulations. The differences found in our
study show that the effects of HU are dose dependent and vary according to their
exposure time.

MEMBROS DA BANCA:
Interno - 1544462 - HERICA DE LIMA SANTOS
Externo à Instituição - JÚLIO ALBERTO MIGNACO - UFRJ
Interno - 1526269 - LEANDRO AUGUSTO DE OLIVEIRA BARBOSA
Externo à Instituição - LUCI MARIA SANT ANA DUSSE - UFMG
Externo à Instituição - MARIA DAS GRACAS CARVALHO - UFMG
Notícia cadastrada em: 07/07/2020 16:57
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