Banca de DEFESA: ADRIANA BENATTI BILHEIRO

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : ADRIANA BENATTI BILHEIRO
DATE: 05/08/2022
TIME: 14:00
LOCAL: Videoconferência
TITLE:

Population characterization of triatomines in four environments in the Western Amazon: gallery forest, alluvial plain, campinarana and pasture.

KEY WORDS:

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PAGES: 76
BIG AREA: Ciências da Saúde
AREA: Saúde Coletiva
SUMMARY:

Triatomines are hematophagous insects, vectors of the protozoan Trypanosoma
cruzi, ethiological agent of Chagas disease. The main transmission occurs in
domestic and peridomestic cycles involving triatomine species and oral
transmission. In recent years it has been observed outbreaks in the Amazon
region mostly by the oral route. The aim of this study is the characterization of
triatomine populations on four different environments on the Brazilian Amazon.
The captures were conducted in four environments and Triatomine specimens
were captured on Attalea speciosa palm trees. The specimens were identified by
morphological and morphometric characters. Molecular analysis were conducted
to identified blood meal sources and genotyping of T. cruzi in 6 DTUs. The
identification of blood meal sources was conducted using a traditional PCR
followed by Sanger sequencing of mtDNA cytb gene. Genotyping of T. cruzi in 6
DTUs was performed based on conventional multilocus PCR and the triatomines
that were positive for T. cruzi and engorged with blood were also targeted for
amplification of the cytB gene for identification of blood-meal sources.
Sequencing was successful in 167 specimens and a total of 21 blood meal
sources were identified: two reptilians, six birds, and 13 mammals. Of the 162
positive samples, the DTUs identified was TCI(87,65%) and TCIV(12,35%). It
was observed that the 102 specimens were engorged with the most varied
bloodmeals. Knowledge of the relationship between triatomines and possible
reservoirs can help to elucidate the enzootic cycle of T. cruzi in the Amazon
region and guide control strategies for Chagas disease transmission in that
region.

BANKING MEMBERS:
Externo à Instituição - SÉRGIO DE ALMEIDA BASANO
Externa à Instituição - GENIMAR REBOUÇAS JULIÃO - FIOCRUZ
Externo à Instituição - JÁDER DE OLIVEIRA - UNESP
Externo à Instituição - JOAO ARISTEU DA ROSA - UNESP
Presidente - 066.589.338-80 - LUIS MARCELO ARANHA CAMARGO - USP