recombinant protein, S. mansoni, diagnostic, schistosomiasis.

The development of a diagnosis test for schistosomiasis performs an important role in the strategy to control and eliminate the disease. Due to the change in the disease epidemiological profile, there is a need to use a diagnostic test more sensitive and specific than the currently available. In a work carried out by our research group, an epitope from the protein Smp_043300.1, described as dopamine receptor, belonging to the rhodopsin family of the GCPRs, was identified and predicted, through the reverse vaccinology technique, with affinity of molecule connection of MHC II human and mice. The linear cell B epitope prediction also was performed with the epitope Sm043300e, which was predicted as cell B epitope. The epitope was synthesized and its immunogenicity was confirmed by its ability to induce the proliferation of TCD4+ cells in sensitized mice by a cocktail of synthetic peptides with selected epitopes by reverse vaccinology. Additionally, this peptide was recognized by antibodies present in the serum of individuals infected with S. mansoni. In this context, it was developed a synthetic gene with a sequence of Sm043300e epitope and a fraction of Smp_043300.1 protein to be used as a target in the serological diagnostic of the schistosomiasis. The recombinant protein sequence was characterized from the

bioinformatic tools and its expression was performed in E. coli. In the in silico analysis, the sequence of the recombinant protein presented 85 amino acids, a theoretical pl of 6.69, a molecular weight of 9,56 kDa and it was predicted as a hydrophilic protein. The prediction analysis of functional domains showed the domains found in the Smp_043300.1 protein were not present in the sequence used to compose the recombinant. The Smp_043300.1(1-43)r protein was expressed in E. coli BL21-Codon Plus, to 37ºC, and in E. coli Arctic Express to 12ªC. The recombinant protein was solubilized using a lysis solution containing 8M of urea and purified by affinity chromatography using imidazole in the washing (40mM) and elution (250mM) stages. The next steps consist in standardize and evaluate its use in a diagnostic test as regards sensibility and specificity using serum of individuals infected or not by S. mansoni.