Banca de QUALIFICAÇÃO: KELLY DE FREITAS SANTOS

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : KELLY DE FREITAS SANTOS
DATE: 26/09/2023
TIME: 09:00
LOCAL: Videoconferência
TITLE:

IT IS MADE ORAL SUPPLEMENTATION WITH PROBIOTICS IN CHILDREN WITH OBESITY IN PRIMARY HEALTH CARE


KEY WORDS:
Child ; Probiotcs; Obesity; Body Weight Changes.

PAGES: 140
BIG AREA: Ciências da Saúde
AREA: Saúde Coletiva
SUMMARY:
Introduction: The intestinal microbiota has been shown to be a potential determining factor
in the development of obesity. There is a dysbiosis of the phyla Bacteroidetes and Firmicutes
in the gut microbiota of individuals with obesity compared to people with normal weight
(eutrophic). There is a lower proportion of the Bacteroidetes phyla. This seems to facilitate the
extraction of energy from ingested food by increasing energy storage in adipose tissue.
Although research with children is still in its infancy, there are indications that the intestinal
microbiota is important for maintaining energy metabolism homeostasis. Objective: To
analyze the effect of oral supplementation with probiotics on nutritional status and associated
variables in children with obesity and severe obesity. Materials and methods: This is a
double-blind, parallel, placebo-controlled clinical trial lasting 12 weeks, with participants
selected at random. The study will consist of a baseline (T0), a 30-day (T30), a 60-day (T60)
and a 90-day (T90) period. The research will be carried out in the 31 Family Health Strategies
(ESFs) in the urban area of the municipality of Divinópolis, Brazil. Participants will be
children of both sexes linked to the ESFs, between the ages of seven and 10 incomplete years
and who are obese or severely obese. Children with a clinical diagnosis of liver disease,
nephropathy, heart disease, cancer, inflammatory bowel disease and those taking probiotic,
prebiotic and symbiotic supplements will not be eligible. Anthropometric and body
composition data, intestinal function, behavior conducive to physical activity and dietary
patterns will be collected. Initially, all the children will be invited to take part in the study (N
= 203). Taking into account the losses that may occur during the process, a sample calculation
will be carried out with a 95% confidence level and 80% test power. This sample calculation
will be based on the pilot study of the present investigation. Participants will be randomized
into two groups, probiotic and placebo. The treatment group will receive probiotic capsules,
each containing Lacticaseibacillus rhamnosus GG (ATCC53103) at a concentration of 1 x
1010 colony-forming units (CFU)/day, for a period of 90 consecutive days. The control group
will receive placebo capsules containing 10mg of gelatine and 10mg of maltodextrin/day for
a continuous period of 90 days. The capsules will be given to the treatment and control groups
at 0, 30 and 60 days. In order to minimize bias in this study, a second researcher, who will not
take part in data collection and analysis, will be responsible for the randomized distribution of
participants into the two groups, indicating and confidentially recording the children who will
receive probiotics or placebo. In addition, the capsules will be packaged equally by the
laboratory, then placed in sealed envelopes by the second researcher and only this researcher vi
will be aware of which participants will receive the probiotic and placebo, indicating the name
of each participant who will receive it. At the end, the data will be tabulated. The pilot study
was carried out between March and July 2023. For this purpose, 20 participants were
considered. In line with the results obtained in the pilot study, specifically the reduction in
waist circumference in children who had probiotic intervention at T0 and T90, the power was
calculated based on the sample obtained (N= 178). An average power of 80% was estimated.
The data collected in the polytomous study was subjected to the Shapiro-Wilk normality test.
The Mann-Whitney non-parametric test for two independent categories and the Kruskal Wallis
non-parametric test for more than two independent categories were used to compare whether
there was a statistically significant difference between the means and medians. Pearson's chi
square test was used for categorical data. The data was expressed as mean ± standard deviation,
median, minimum and maximum, Q1 (first quartile) and Q3 (third quartile). A 5% significance
level was adopted. Preliminary Results: We analyzed information from a total of 20 children,
whose average age was 9 years (± 1.03). With regard to the anthropometric data of the children
investigated, body weight did not vary between the evaluations, being 49.0 kg (±12.44 -
12.74). On the other hand, there was a slight reduction in waist circumference, especially
between the first 76.50 (±10.18) and the last 75.25 (±10.15) assessments. As well as the body
mass index, at the first assessment 2.99(±1.07) and at the fourth assessment 2.80(±1.08), not
changing the nutritional status of obesity. In relation to the daily consumption of in natura or
minimally processed, processed and over-processed foods, there was no difference in intestinal
functioning (slow, adequate or fast transit) based on the consumption of these food groups (p
> 0.05). In terms of intestinal function, as measured by intestinal transit time, there was no
difference in the intestinal transit of those who received the probiotic or placebo (p = 0.806).
Partial Consideration: The results of this investigation could contribute to the understanding
of the effects of probiotic supplementation on the modulation of intestinal microbiota in
children with obesity, in addition to contributing to the current literature in the field of child
and adolescent health. Registered in the Brazilian Registry of Clinical Trials (ReBEC)–RBR-
9qg9qhh.

BANKING MEMBERS:
Externa à Instituição - LUANA CAROLINA DOS SANTOS - UFMG
Presidente - 1347706 - ALBA OTONI COLLARES
Externa à Instituição - NAYARA RAGI BALDONI COUTO - UIT
Notícia cadastrada em: 04/09/2023 07:54
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