Banca de QUALIFICAÇÃO: JÉSSICA ALVES FARIA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : JÉSSICA ALVES FARIA
DATE: 30/11/2023
TIME: 09:30
LOCAL: Sala 302 - Bloco C - CCO
TITLE:

Assessment of the Neuroprotective Activity of the Digoxin Derivative (BD-15) in the Cortex Pre-frontal view of Wistar rats subjected to neuroinflammation


KEY WORDS:
neuroinflammation; oxidative stress; lipopolysaccharides; BD-15; prefrontal cortex; neuroprotection.

PAGES: 51
BIG AREA: Ciências da Saúde
AREA: Farmácia
SUMMARY:
The neuroinflammatory process and oxidative stress have been associated with the
neurodegenerative diseases and disorders, such as anxiety and depression. Cardiotonic
steroids, such as ouabain and digoxin, have stood out for being able to attenuate
neuroinflammation and oxidative stress, in namolar concentrations. In this context, the present
project evaluated the effects of the new semisynthetic derivative of the cardiotonic steroid
digoxin, BD-15, on the prefrontal cortex of male Wistar rats, subjected to a induced
lipopolysaccharides neuroinflammation. For this, this project, approved by the UFSJ Animal
Use Ethics Committee (protocol 7812090522), divided the animals into four experimental
groups: control (0.9% NaCl), LPS (250µg/kg), BD-15 ( 100µg/kg), and LPS (250µg/kg),
+BD-15 (100µg/kg). The injections were via intraperitoneal, in the morning. Two hours after
the last injection, the animals were subjected to open field behavioral testing and then
euthanized. The prefrontal cortex was removed for subsequent biochemical and statistical
analysis. The behavioral results demonstrated that the LPS group had a 55% increase in the
immobilization time of the animals, in addition to an 83% reduction in the number of visits to
the central quadrants, in relation to the control group. Biochemical analyzes revealed that, in
the LPS+BD-15 group, BD-15 was able to increase GSH levels by approximately 30% in
relation to the LPS group, which had a reduction of, approximately, 27% when compared to
the control. Lipid peroxidation levels showed that BD-15 was also able to reverse the damage
generated by LPS, reducing peroxidation levels by approximately 38% when compared to the
LPS group. Regarding Na,K-ATPase activity, no significant difference was found between the
different groups. Thus, the results indicate that BD-15 was able to protect the animal's
behavior from the effects caused by LPS, in the LPS+BD-15 group, as well as reducing the
levels of lipid peroxidation and increasing those of GSH. Therefore, the results encourage the
carrying out of new experiments aimed at elucidating the effects of BD-15 in inflammatory
and oxidative stress conditions, as it has shown a potential neuroprotective effect.

BANKING MEMBERS:
Presidente - 1544462 - HERICA DE LIMA SANTOS
Interna - 1945166 - MAIRA DE CASTRO LIMA
Externa ao Programa - 1106384 - LUCIANA ESTEFANI DRUMOND DE CARVALHO
Externa à Instituição - GABRIELA MACHADO PARREIRA - FARMAX
Notícia cadastrada em: 27/11/2023 07:26
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