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Banca de DEFESA: JÉSSICA ALVES FARIA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : JÉSSICA ALVES FARIA
DATE: 07/02/2024
TIME: 09:30
LOCAL: Videoconferência
TITLE:

EVALUATION OF BEHAVIORAL PARAMETERS AND BIOCHEMISTRY IN THE FRONTAL CORTEX OF WISTAR RATS SUBJECTED TO NEUROINFLAMATION BY LIPOPOLYSACCHARIDES (LPS) AND TREATED WITH THE DIGOXIN DERIVATIVE (BD-15)

KEY WORDS:

oxidative stress; lipopolysaccharides; cardiotonic steroids; BD-15; Na,K-ATPase; frontal cortex; neuroprotection.

PAGES: 67
BIG AREA: Ciências da Saúde
AREA: Farmácia
SUMMARY:

The neuroinflammatory process and oxidative stress have been associated with the

neurodegenerative diseases and disorders, such as anxiety and depression. Cardiotonic steroids

have stood out for being able to attenuate neuroinflammation and oxidative stress, in namolar

concentrations. In this context, the present project evaluated the effects of the new semisynthetic

derivative of the cardiotonic steroid digoxin, BD-15, on the frontal cortex of male Wistar rats,

subjected to a induced-lipopolysaccharides neuroinflammation. For this, this project, approved

by the UFSJ Animal Use Ethics Committee (protocol 7812090522), divided the animals into

four experimental groups: control (0.9% NaCl), LPS (250µg/kg), BD-15 (100µg/kg), and LPS

(250µg/kg), +BD-15 (100µg/kg). The injections were via intraperitoneal, in the morning. Two

hours after the last injection, the animals were subjected to open field behavioral testing and

then euthanized. The frontal cortex was removed for subsequent biochemical and statistical

analysis. The behavioral results demonstrated that the LPS group moved less around the

apparatus (55%), resulting in an 83% reduction in the number of visits to the central quadrants,

in relation to the control group. Biochemical analyzes revealed that, in the LPS+BD-15 group,

BD-15 was able to increase GSH levels by 30% in relation to the LPS group, which had a

reduction of 27% when compared to the control. Lipid peroxidation levels showed that BD-15

was also able to reverse the damage generated by LPS, reducing peroxidation levels by

approximately 38% when compared to the LPS group. Regarding Na,K-ATPase activity, no

significant difference was found between the different groups. Thus, the results indicate that

BD-15 was able to protect the animal's behavior from the effects caused by LPS, in the

LPS+BD-15 group, as well as reducing the levels of lipid peroxidation and increasing those of

GSH. Therefore, the results encourage the carrying out of new experiments aimed at elucidating

the effects of BD-15 in inflammatory and oxidative stress conditions.

BANKING MEMBERS:
Presidente - 1544462 - HERICA DE LIMA SANTOS
Externa à Instituição - JACQUELINE ALVES LEITE - UFG
Externa ao Programa - 1106384 - LUCIANA ESTEFANI DRUMOND DE CARVALHO
Interna - 1945166 - MAIRA DE CASTRO LIMA

Notícia cadastrada em: 22/01/2024 10:41