Banca de DEFESA: DAIANE DULCILEIA MORAES DE PAULA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : DAIANE DULCILEIA MORAES DE PAULA
DATE: 16/12/2022
TIME: 14:00
LOCAL: meet.google.com/tog-zyxv-bup
TITLE:

Enantioselective determination of tramadol from plasma by capillary electrophoresis using molecularly imprinted polymer as adsorbent in magnetic solid phase extraction

KEY WORDS:

capillary electrophoresis, tramadol, magnetic solid phase extraction, molecularly imprinted polymers, restricted access material.

PAGES: 85
BIG AREA: Ciências Exatas e da Terra
AREA: Química
SUBÁREA: Química Analítica
SUMMARY:

Tramadol (TRM), an opioid analgesic that is marketed as a racemic mixture of two enantiomers (1R, 2R) and (1S, 2S), is used to treat moderate to severe acute and chronic pain and is being monitored by the World Anti-Doping Agency. In this work, an enantioselective analytical method was developed by capillary electrophoresis using sulfated beta-cyclodextrin. In addition, a molecularly imprinted magnetic restricted-access polymer double coated with hydrophilic monomer and bovine serum albumin have been synthtized which was properly characterized, presenting consistent and indicative results that the double coating was well performed. This adsorbent was effectively used in magnetic solid phase extraction, and several parameters were optimized, such as: sample pH, sample volume, elution solvent, elution solvent volume, stirring time, washing solvent, amount of material and reuse, obtaining reproducible recoveries close to 100%. The analytical method was validated according to ANVISA resolution 27/2012, obtaining linearity in the range of 100-3000 ng mL-1, in addition to being selective, precise and exact, it presented LOQ of 100 ng mL-1 for both enantiomers, which allowed its determination in real plasma samples, being an important alternative in the monitoring of athletes and forensic area.

BANKING MEMBERS:
Presidente - 1872495 - KEYLLER BASTOS BORGES
Externo à Instituição - MARCONE AUGUSTO LEAL DE OLIVEIRA - UFJF
Externa ao Programa - 1348442 - PATRICIA BENEDINI MARTELLI