Banca de QUALIFICAÇÃO: DAIANE DULCILEIA MORAES DE PAULA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : DAIANE DULCILEIA MORAES DE PAULA
DATE: 03/12/2021
TIME: 14:00
LOCAL: REMOTO
TITLE:

Enantioselective determination of tramadol in plasma by capillary electrophoresis employing molecularly imprinted polymer as adsorbent in magnetic solid phase extraction

KEY WORDS:

Magnetic solid phase extraction, molecularly imprinted polymer, restricted access material, enantiomer separation

PAGES: 30
BIG AREA: Ciências Exatas e da Terra
AREA: Química
SUBÁREA: Química Analítica
SUMMARY:

Tramadol (TRM) is an opioid analgesic used from moderate to severe pain, its use may have side effects such as nausea, drowsiness and concentration loss. The drug is marketed as a racemic mixture of two enantiomers (1R, 2R) (1S, 2S). From the above, two analytical methods by capillary electrophoresis (CE) were developed for the TRM determination in human plasma, one of them non-enantioselective and the other enantioselective using S-β-CD. In addition, a restricted access magnetic molecularly imprinted polymer (MMIP) double coated with hydrophilic monomer and bovine serum albubbin (BSA) named RA-MMIP-HM-BSA was successfully synthesized and it was used effectively in MSPE. In sample preparation, several MSPE parameters were optimized, such as: pH and volume sample, elution solvent and  its volume, stirring time, wash solvent, material amount and reuse obtaining TRM recoveries reproducible and close to 100%. The analytical method was validated obtaining linearity in the range of 100-3000 ng mL-1, in addition to being selective, precise and accurate, presenting a LOQ of 100 ng mL-1 for both enantiomers, which makes it possible its determination on real samples.

BANKING MEMBERS:
Externo à Instituição - LEANDRO AUGUSTO CALIXTO - UNIFESP
Externa ao Programa - 1348442 - PATRICIA BENEDINI MARTELLI