Banca de DEFESA: DANIEL FERREIRA VIDAL

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : DANIEL FERREIRA VIDAL
DATE: 17/12/2021
TIME: 08:30
LOCAL: REMOTO
TITLE:

Determination of β-agonist ractopamine residues in food matrices using magnetic solid phase extraction with molecularly imprinted polymers and capillary electrophoresis

KEY WORDS:

Ractopamine, molecularly imprinted polymers, magnetic solid phase extraction, capillary electrophoresis

PAGES: 71
BIG AREA: Ciências Exatas e da Terra
AREA: Química
SUBÁREA: Química Analítica
SUMMARY:

Ractopamine (RAC) is a food additive widely used in animal production whose main objective is to enhance meat production and improve product quality. However, the indiscriminate use of this growth stimulant can lead to the production of meats with a high level of residue from this drug, which can pose a health risk in addition to arousing the attention of more demanding consumers. Based on this premise, a simple and fast method was developed for the determination of RAC in food samples of animal origin, using magnetic solid phase extraction (MSPE) with molecularly printed polymer with restricted access, and capillary electrophoresis (CE). Firstly, it was performed the synthesis of magnetic nanoparticles by the method of co-precipitation of Fe2+ and Fe3+ ions in a basic medium, and these particles were later functionalized with tetraethylorthosilicate. Then, the imprinted polymer was synthesized on the surface of the functionalized magnetic particle by the precipitation method, using RAC as template, acrylic acid as functional monomer, ethylene glycol dimethacrylate as crosslinking agent and benzalkonium chloride as surfactant. Then the magnetic MIP was double coated, firstly with the hydrophilic monomers 2-hydroxyethyl methacrylate and glycerol dimethacrylate and then with bovine serum albumin, in order to transform the MIP into an adsorbent with restricted access. The characterization of the synthesized material was carried out by the techniques of DRX, FTIR, TGA, SEM, TEM, wettability test, zero charge point tests, presenting results consistent with the synthesis processes and suggesting that the double coating on the imprinted polymer was successful. For the analysis and quantification of RAC, a reverse injection CE method was optimized, which showed to be very promising for ultra-fast RAC analysis, since the analyte migration times were less than 1 min and the analytical signals obtained had good peak asymmetry, and peaks free from interfering and/or baseline noise. The MSPE showed good applicability, since, in addition to the ease of application, after the optimization process it was possible to reach recovery values of the analyte close to 90% in samples of bovine milk, bovine muscle and muscle pig. The method validation process showed satisfactory results, and the parameters related to selectivity, analytical curve quality, precision, accuracy and robustness of the method that were found are within acceptable limits according to ANVISA resolution 166/2017. The developed method was then applied to real samples of bovine milk and bovine and porcine muscle, with a
iv relative error being observed for fortified samples of less than 15%, mainly in porcine muscle samples, where it was possible to observe an electropherogram with less interference compared to to the electropherograms referring to the other matrices.

BANKING MEMBERS:
Presidente - 1872495 - KEYLLER BASTOS BORGES
Externa à Instituição - LEANDRA DE OLIVEIRA CRUZ DA SILVA - IFSUDESTEMG
Externa ao Programa - 1348442 - PATRICIA BENEDINI MARTELLI