Purification of fungal alpha-galactosidase enzyme and evaluation of its applications for oligosaccharide hydrolysis in soybean derivatives
Biocatalyst; immobilization; application in soy products
The α-D-Galactosidase enzyme is responsible for the hydrolysis of the terminal galactose bond in complex oligosaccharides, converting them into simpler sugars such as glucose and galactose. This property makes the enzyme to be used in the treatment of non-nutritive α-galacto-oligosaccharides (GOS), recognized and used in the treatment of soy-based products, which have high concentrations of these oligosaccharides. If ingested in large quantities, they can cause stomach pain, diarrhea and flatulence. This study aims to extract, semi-purify and immobilize the enzyme from Trichoderma virens for characterization and use in the treatment of soy milk.