Banca de DEFESA: GRAZIELE MAYRA SANTOS MOREIRA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : GRAZIELE MAYRA SANTOS MOREIRA
DATE: 22/07/2022
TIME: 14:00
LOCAL: On-line
TITLE:

HISTOMORPHOMETRIC ANALYSIS OF THE CARDIORESPIRATORY TISSUE OF RATS WITH PARKINSON'S DISEASE TRAINED WITH HIGH INTENSITY PHYSICAL EXERCISE

KEY WORDS:

Parkinson’s. Functional Performance. High Intensity Interval Training. Heart. Respiratory Muscles.

PAGES: 52
BIG AREA: Ciências Biológicas
AREA: Morfologia
SUMMARY:

Introduction: Parkinson's Disease (PD) affects the cardiorespiratory system causing greater left ventricular mass in the heart and respiratory muscle weakness compared to healthy individuals. Objective: Analyze the histomorphometric alterations of cardiac tissue and respiratory muscles, in addition to the functional performance of rats with PD submitted to TIAI. Materials and Methods: We used 70 male Wistar rats, 40 days old, divided into groups with Parkinson's (PA) and Sham (SH); subdivided into groups that performed High Intensity Interval Training Before Surgery (TIAIa); TIAI After Surgery (TIAId) and TIAI Before and After Surgery (TIAIad). Physical exercise was performed before and/or after PD induction. The rats performed the exercise, 5 times a week, for 25 minutes/day, for 4 or 8 weeks. The functional performance of the animals was evaluated by of false step and parallel bars tests. For morphometric analysis of the heart, the relative weight of the heart, diameter and thickness of the left ventricle were calculated. The histochemistry of the substantia nigra of the midbrain was performed using the Nissl method. Myocardial, diaphragm, intercostal and rectus abdominis muscles were stained with Hematoxylin and Eosin (HE). The histomorphometric study was performed with the Image J program to analyze the cross-sectional area of the muscles and the number of muscle fibers. Data analysis was performed using the GraphPad Prism 9.3 statistical program, one-way ANOVA test, followed by Tukey's post-hoc test (p<0.05). Results: The analysis of the hind legs with the parallel bars test showed that the trained animals showed improvement in functional performance compared to the animals in the sedentary group. The relationship between the body weight, heart weight and number of cardiomyocytes showed no significant difference in PA and SH trained animals. There was an increase in the areas of the diaphragm, intercostal and rectus abdominis muscles in the PA and SH trained groups compared to the sedentary ones. Conclusion: The TIAI provided hypertrophy of the respiratory muscles and left ventricle of the heart, in addition to improving the functional performance of the hind legs of animals with PD.

BANKING MEMBERS:
Externo à Instituição - ANTONIO JOSE NATALI - UFV
Interna - 1742558 - FLAVIA CARMO HORTA PINTO
Interna - 1811013 - LAILA CRISTINA MOREIRA DAMAZIO
Presidente - 1348432 - LILIAM MIDORI IDE