Banca de DEFESA: SILVANA VENANCIO DA SILVA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : SILVANA VENANCIO DA SILVA
DATE: 29/07/2022
TIME: 14:00
LOCAL: On-line
TITLE:

The effect of high-intensity physical exercise on neuronal density and astrocyte expression before and after Parkinson's disease induction in rats

KEY WORDS:

Parkinson Disease, astrocytes, exercise, neuroprotection, substantia nigra, corpus striatum

PAGES: 52
BIG AREA: Ciências Biológicas
AREA: Morfologia
SUMMARY:

Parkinson's disease (PD) affects dopaminergic neurons located in the pars compacta of the substantia nigra. The practice of resistance physical activity is a non-pharmacological intervention that has been tested in the treatment of PD. Thus, the present project aimed to investigate the effects of high-intensity exercise on neuronal density and astrocyte expression in the brain of rats with PD that exercised before and after disease induction. Seventy Wistar rats were used, distributed as follows: 09 animals trained before PD induction (DP-Exa), 09 trained after PD induction (DP-Exd); 10 trained before and after PD induction (DP-Exad) and 09 sedentary animals (DP-Sed). The same groups were distributed to the group without PD (Sham). High-intensity physical training was performed on the vertical ladder before and/or after PD induction. It was performed for 5 days/week, 30 to 45 minutes, for 4 weeks. PD induction was performed using the electrolytic lesion model in the coordinates: AP equal to -4.9, ML equal to 1.7 and DV equal to 8.1. The animals' motor performance was evaluated at the beginning and at the end of training with false step the test, parallel bars test and open field tests. At the end of the experiment, the brain was removed for histochemistry, staining by the Nissl method, and immunohistochemistry by expression of Glial Fibrillary Acid Protein (GFAP) from the substantia nigra and striatum. The histomorphometric study was performed with the Image J program for counting neurons and astrocytes (GFAP). The GraphPad Prism 9.3 program was used, one-way ANOVA test, followed by Tukey's post-hoc (p<0.05). The analysis showed better performance of the animals that trained compared to the sedentary group, in the parallel bars and open field tests. Data on neuron counts in the striatum, substantia nigra and GFAP in the animals of the DP-Exa, Sham-Exa, DP-Exad and Sham-Exad groups showed a greater number of neurons and greater expression of GFAP, when compared to the groups DP-Exd, Sham-Exd, DP-Sed and Sham-Sed. in conclusion, the groups of animals that performed high-intensity training before, before and after PD induction had higher densities of neurons and astrocytes.

BANKING MEMBERS:
Interna - 027.818.286-02 - ETEL ROCHA VIEIRA - UFVJM
Externa à Instituição - ITA DE OLIVEIRA SILVA
Presidente - 1811013 - LAILA CRISTINA MOREIRA DAMAZIO
Interno - 2141553 - PAULO HENRIQUE DE ALMEIDA CAMPOS JUNIOR