Banca de DEFESA: ALISSON KENNEDY REZENDE

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : ALISSON KENNEDY REZENDE
DATE: 22/07/2022
TIME: 13:30
LOCAL: Sala C.314, DCNAT, Campus Dom Bosco/UFSJ, Pça Dom Helvécio 74, SJDR - Formato híbrido
TITLE:

Systemic effects of oral tolerance in Wistar rat healing bone repair on remodeling and calcification phase

KEY WORDS:

bone repair, oral tolerance, zein, biomechanics

PAGES: 121
BIG AREA: Ciências Biológicas
AREA: Morfologia
SUMMARY:

In addition to vitamins, carbohydrates, and fiber, the corn present in our diet offers
protein varieties such as zein. This protein, after contact with the immune system
via the gastrointestinal tract, is capable of inducing immunological antigen
tolerance. Studies have shown that re-exposures of previously ingested proteins
can improve the repair of skin wounds, with a decrease in granulomas, among
other systemic effects. However, there are still no records of systematic
tolerogenic effects of oral tolerance on post-injury bone tissue repair. Therefore,

this study aimed to evaluate the systemic effects of zein protein tolerized and re-
exposed parenterally on the bone repair process (28 and 45 days) after

osteotomy in rat tibiae. Twelve-week-old male Wistar rats were used and divided
into three experimental groups, with i.p. application of Saline (GS), Adjuvant
(Al(OH)3) (GA), and Zeine associated with the adjuvant (GZ). A 2 mm osteotomy
was performed on the right tibiae, and at 28 and 45 days post defect
histomorphometric analyses, bone densitometry (BMD), and biomechanical tests
were performed. The weight monitoring of both the weight and the osteomized
tibiae showed no changes between the groups. In the BMD analysis, we
observed significant differences between the GS and GA groups. There was no
difference between the experimental time of the GS, suggesting that bone
mineralization is greater on day 28 after the defect. The biomechanical shear
stress test performed on the right tibiae showed a significant difference only in
the maximum force variable of the GS, indicating that at 45 days after the defect
the tissue became more resistant to external mechanical forces. In the
histological evaluation, we observed acceleration of the repair process in the
ZMG compared to the other groups at 28 days post-defense and similarity
between all groups at 45 days post-defense. The results of the present study
demonstrate the beneficial and systemic action of oral tolerance, after
immunization by previously ingested protein, in the phases of ossification and
bone remodeling. Further studies are necessary to elucidate the mechanisms
involved and to generate new future therapeutic forms.

BANKING MEMBERS:
Externa à Instituição - GERLUZA APARECIDA BORGES SILVA - UFMG
Externo ao Programa - 2362132 - ALVARO CESAR DE OLIVEIRA PENONI
Presidente - 2279745 - ERIKA LORENA FONSECA COSTA DE ALVARENGA
Interna - 2104326 - RAQUEL ALVES COSTA