Banca de DEFESA: ELISANGELA ELDUINA FERREIRA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : ELISANGELA ELDUINA FERREIRA
DATE: 22/08/2022
TIME: 14:00
LOCAL: On-line
TITLE:

Effects of the use of ethanolic extract and hydroethanolic fraction of the ripe fruit of Solanum lycocarpum on the healing process of skin wounds in mice

KEY WORDS:

Wound healing, medicinal plants, Solanum

PAGES: 87
BIG AREA: Ciências Biológicas
AREA: Morfologia
SUMMARY:

Plant species are a rich source for the discovery of active principles and the development of
new therapies. The species Solanum lycocarpum A.St.-Hil has antioxidant, anti-inflammatory
and antimicrobial effects confirmed in some experimental models. In addition, several
phytochemicals present in the extracts used were found in other species of the genus, such as
some phenolic compounds, which were related to the effects of improving the healing
process, with individual or synergistic effects on one or more stages of wound healing. The
research project aimed to analyze the effects of the use of ethanolic extract and the
hydroethanolic fraction of the ripe fruit of Solanum lycocarpum on the healing process of skin
wounds in mice. Seventy-two animals were randomly divided into three groups, a Control
Group that received topical application of physiological saline solution, a group treated with
ethanol extract and a group treated with the hydroethanolic fraction of the ripe fruit of
Solanum lycocarpum. Each group was subdivided into four other subgroups according to the
treatment period: 1, 5, 7 and 21 days, with n=6 in each subgroup. To make the excisional
wounds, a metallic dermatological punch, 7 mm in diameter, was used. Macroscopic
evaluations of each lesion were performed daily to verify the presence of inflammatory signs
(edema, hyperemia), the presence of crusts and secretions and to calculate the rate of
contraction of the wounds. After 1, 5, 7 or 21 days after the excisional lesion, the animals
were euthanized by a lethal dose of anesthetic. A fragment containing the wound was
removed, cleaved and fixed in a 10% neutral buffered formalin solution. Subsequently, the
material was histologically processed and stained by histological and histochemical methods,
for histopathological and morphometric evaluation of the inflammatory infiltrate, fibroblasts,
re-epithelialization and collagen deposition, after daily topical treatment with ethanolic extract
of the ripe fruit of Solanum lycocarpum and its hydroethanolic fraction. The results show that
macroscopically the treatments did not interfere with the closure of excisional wounds over
the 21 days. Topical application of ethanolic extract from the ripe fruit of Solanum
lycocarpum reduced the inflammatory infiltrate and areas of edema after 1, 5 or 7 days of treatment. No differences were observed in fibroblast, mast cell and blood vessel counts. In the treated groups, the remodeling process was in progress, however, a better evolution was observed in the group that received ethanol extract, where the epithelium was more similar to that of the areas without lesion.

BANKING MEMBERS:
Presidente - 1742558 - FLAVIA CARMO HORTA PINTO
Externa ao Programa - 1540495 - LUCIANA ALVES RODRIGUES DOS SANTOS LIMA
Externa à Instituição - PAULA VIEIRA TEIXEIRA VIDIGAL - UFMG
Interno - 2141553 - PAULO HENRIQUE DE ALMEIDA CAMPOS JUNIOR