Banca de QUALIFICAÇÃO: KARINE STHÉFANY SERPA AMARAL DIAS

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : KARINE STHÉFANY SERPA AMARAL DIAS
DATE: 11/07/2019
TIME: 13:30
LOCAL: UFSJ - São João del-Rei
TITLE:

Effects of use ethanolic extract of Ocimum basilicum in the healing process of skin 

wounds in mice

KEY WORDS:

Keywords: healing, skin wounds, Ocimum basilicum, anti-inflammatory, treatment.

PAGES: 24
BIG AREA: Ciências Biológicas
AREA: Morfologia
SUBÁREA: Histologia
SUMMARY:

The healing of skin wounds is a process that involves a serie of events, cells and factors, aimed at repairing the tissue that initially suffered a trauma or injury. Many researches are currently focused on the serach for possible therapies and drugs that can contribute to the repair process. Medicinal plants are the subject of much research, given their range of pharmacological properties, including studies of their use in wounds in experimental models. Basil (Ocimum basilicum) is a plant that has been described with many properties, including anti-inflammatory and antioxidante. The aim of the presente work was to analyze the effects of intraperitoneal (i.p.) and the topical administration of the ethanolic extract of Ocimum basilicum (EEOB) in the healing process of incisional and excision cutaneous wounds in mice. Swiss mice were used, divided into groups according to treatment and type of wound. In the animals that made the incisional wound, the treatment was performed with intraperitoneal EEOB (i.p.) at 150 mg/Kg daily or saline i.p., for 5 or 7 days post-injury, depending on the group, and later the animals were euthanized. In the animals that made the excision wounds, the treatment was carried out in the groups treated with topical EEOB at 38 mg/Kg in the left lesion and topical EEOB at 100 mg/Kg in the right lesion, three times a day, while the control group received topical saline; the animals were subsequently euthanized 1, 3, 5 or 21 days after injury and treatment, depending on the experimental group. Thereafter, the lesions underwent histological processing and microscopic analysis, and the excisional lesions were also assessed macroscopically before euthanasia. Histopathological analyzes in animals with incisional lesion and treated with EEOB injection i.p. in relation to the group that received saline i.p. daily, point to a decrease in the number of leukocytes at 5 days and mast cells at 7 days post-injury. Regarding excision wounds, the analysis indicates that within 1 day there was a decrease in the number of leukocytes in the treated groups in relation to the saline group and a decrease in the area of edema and adipose tissue. At 3 days, the grouptreated with EEOB at 100 mg/Kg showed a larger area of macroscopic wound compared to the other groups. At 5 days, there was a decrease in new blood vessels in the EEOB treated group at 38 mg/Kg compared to saline; and the EEOB group at 100 mg/Kg had less adipose tissue compared to the other groups and a greater area of granulation tissue. Finally, at 21 days, reepithelization was complete in most animals of all groups, and collagen deposition in the scar region was better in the treated groups, with more similarity to the intact skin adjacent to the lesion area. The results point to an antiinflammatory action of EEOB in the groups that received the extract, mainly in the initial phase of the process. Furthermore/besides, the extract may have influenced the better collagen deposition in the tissue remodeling phase.

BANKING MEMBERS:
Interna - 2279745 - ERIKA LORENA FONSECA COSTA DE ALVARENGA