Banca de DEFESA: RENAN DINIZ FERREIRA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : RENAN DINIZ FERREIRA
DATE: 30/08/2023
TIME: 14:00
LOCAL: Sala C.314, DCNAT, Campus Dom Bosco/UFSJ, Pça Dom Helvécio 74, SJDR - Formato híbrido
TITLE:

Effects of topical use of Ageratum conyzoides essential oil on the wound healing process skin in mice

KEY WORDS:

wound healing; essential oil; Ageratum conyzoides

PAGES: 87
BIG AREA: Ciências Biológicas
AREA: Morfologia
SUMMARY:

In humans, tissue repair can occur in two ways: by regeneration or by healing, where
there is loss of tissue functionality. In an attempt to make this process more efficient,
products of natural origin have become an alternative to improve wound healing.
Ageratum conyzoides (AC) is popularly known as "Erva de São João" and
traditionally used as an anti-inflammatory, analgesic and to relieve menstrual and
intestinal cramps. Studies report that the use of the ethanolic extract of this herb
increases the cell proliferation of keratinocytes and collagen synthesis. Other studies
also indicate that methanolic extract induces wound contraction. Therefore, the aim
of the present study was to evaluate the topical effect of Ageratum conyzoides
essential oil on the healing process of excisional cutaneous wounds in mice. For
this, 72 male mice were randomly distributed into four groups: (i) saline group, which
received topical application of physiological saline; (ii) DMSO group, which received
topical application of DMSO; (iii) TOX group, which received topical application of AC
essential oil diluted in DMSO, at a concentration of 0.01mg/10000μl; and (iv) TOY
group, which received topical application of AC essential oil diluted in DMSO, at a
concentration of 0.001mg/10000μl. Each group was subdivided into three other
subgroups, at times of 1, 5 and 7 days of treatment, with n = 6. In addition, 18 more
male mice were randomly distributed into three groups: DMSO, TOX and TOY,
following the same treatments previously described, in the time of 28 days. The
excisional wounds were induced, the animals treated, and after each treatment
period, the lesions were collected, processed and analyzed macroscopically and
microscopically, through histological, histochemical and immunohistochemical
techniques, for histopathological and morphometric evaluation of: inflammatory
infiltrate, re-epithelialization and crusts, mast cells, new blood vessels, deposition of
collagen fibers and macrophages. The results show that macroscopically, at the end
of seven days, the area of the lesion in the TOX group was significantly smaller than
in the saline-treated group and the TOY group. Topical application of AC essential oil
reduced the inflammatory infiltrate, after 1, 5 and 7 days of treatment and after one
day, fibroblastic cells and macrophages were already observed in the area of
lesions. At 28 days of treatment, there was an increase in the number of mast cells
and better deposition of collagen fibers. Thus, it is concluded that the topical
application of the essential oil of Ageratum conyzoides interferes positively in the
wound healing process.

BANKING MEMBERS:
Externo à Instituição - WANDERSON GERALDO DE LIMA
Presidente - 1742558 - FLAVIA CARMO HORTA PINTO
Interna - 1811013 - LAILA CRISTINA MOREIRA DAMAZIO
Interna - 2144084 - PRISCILA TOTARELLI MONTEFORTE