Banca de DEFESA: ANA PAULA ÁLVARES DA SILVA RAMOS

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : ANA PAULA ÁLVARES DA SILVA RAMOS
DATE: 15/12/2023
TIME: 14:00
LOCAL: SALA 3.14C- DCNAT
TITLE:

CHARACTERIZATION OF THE PROFILE OF CIRCULATING MICROVESICLES
IN THE BLOOD OF PATIENTS WITH EPITHELIAL OVARIAN CANCER: A
LIQUID BIOPSY STRATEGY.

KEY WORDS:

Ovarian cancer, Liquid biopsy, Microvesicles, Biomarkers.

PAGES: 48
BIG AREA: Ciências Biológicas
AREA: Genética
SUBÁREA: Genética Humana e Médica
SUMMARY:

Ovarian cancer (OC) is the second most common neoplasm related to the female
reproductive system. Currently, precision medicine in oncology has sought earlier and
more precise diagnostic methodologies, since a better understanding of aspects of tumor
growth dynamics considerably increases the chances of obtaining more satisfactory
results in the therapeutic approach. Personalized treatment, however, requires
increasingly specific diagnostic techniques, early detection of the disease and real-time
monitoring of tumor development, all of this, combined with less invasive procedures,
without exposing the patient to clinical risks. of a surgical biopsy. In this context,
extracellular vesicles (EVs) have demonstrated an interesting potential for a liquid
biopsy approach, since the characterization of microvesicles (MVs) circulating in
peripheral blood has been presented as a promising alternative for understanding the
dynamics of the development of tumors in various types of cancer. This study carried
out the phenotypic characterization of subpopulations of circulating microvesicles
(MVs) originating from cells of the immune system and tumor cells (CTCs) by flow
cytometry, evaluating their therapeutic potential as biomarkers for the diagnosis of
epithelial ovarian cancer (EOC). Peripheral blood was collected from 9 volunteers
without detectable ovarian disease (Control group) and from 21 patients with DSC. The
data demonstrated that patients with OC have a greater number of circulating MVs than
women without evidence of ovarian disease. Furthermore, the results obtained
demonstrate that MVs derived from eosinophils, platelets and CTCs-EpCAM are
biomarkers capable of identifying patients with EOC through peripheral blood with the
potential to be used in screening and diagnostic campaigns, in association with dosages.
of serum CA125 values. The data indicate that the combined use of measurements of
serum CA125 values associated with measurements of CTCs-EpCAM MVs can classify
patients as early, advanced, or free stage of epithelial ovarian cancer.

BANKING MEMBERS:
Interna - 1742558 - FLAVIA CARMO HORTA PINTO
Externo à Instituição - JORGE GOMES GOULART FERREIRA
Interna - 864.990.846-20 - LETICIA DA CONCEIÇÃO BRAGA - IZABELA HENDRIX
Interna - 2144084 - PRISCILA TOTARELLI MONTEFORTE