Banca de DEFESA: MONIQUE MACEDO COELHO

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : MONIQUE MACEDO COELHO
DATE: 01/02/2021
TIME: 09:00
LOCAL: GOOGLE MEET-meet.google.com/ign-nujr-oie
TITLE:

EVALUATION OF THE ASSOCIATION OF GOLD NANOPARTICLES TO OVALBUMIN AS ADJUVANT IN IMMUNE RESPONSE

KEY WORDS:

adjuvant

gold nanoparticle

oral tolerance

PAGES: 109
BIG AREA: Ciências Biológicas
AREA: Imunologia
SUBÁREA: Imunologia Aplicada
SUMMARY:

There are several proposals for interferences in the immune response, either preventive or therapeutic. One of these very promising forms of interference that has been studied is the injection of immunologically tolerated proteins. Oral tolerance is a phenomenon that inhibits the immune response, such as the formation of specific antibodies, after immunization with antigen plus adjuvant in animals previously exposed to the protein via the oral route. In this work, we studied whether subcutaneous application (s.c.) in mice, of gold nanoparticles (AuNPs) associated with Ovalbumin (OVA) would contribute to the establishment of oral tolerance, as an adjuvant material. In this sense, we synthesized spherical AuNPs, with approximately 7 nm, functionalized with OVA and made the physical-chemical characterization of the material, for that, we used the Ultraviolet-Visible Spectroscopy (UV-Vis), Transmission Electron Microscopy (MET), Scattering Dynamic Light (DLS) and the Zeta Pontential. Subsequently, an oral treatment with OVA was performed for 5 consecutive days in Swiss, male, 8-week-old mice. Seven days after oral treatment, all animals received their primary injections (s.c) at the base of the tail and twenty-one days after the primary injections, the animals received secondary injections (s.c). On the day of the primary injections, lesions were made in one of the mice's ears. We evaluated the immunization potential, as well as the establishment of the OVA tolerance of the animals, verifying the total anti-OVA antibody titer with the Enzyme-linked Immunosorbent Assay (ELISA). The base of the tail was also histologically evaluated, at the place where the animals received injections and ear injuries, to assess the indirect effects of oral tolerance. The results demonstrated that AuNP + OVA have an adjuvant role and are able to maintain the indirect effects of oral tolerance. Subsequently, to understand
16
which intracellular signaling pathways AuNPs and AuNPs + OVA can interfere with immune responses, we did a modeling in silico interactions between AuNPs and AuNPs + OVA with OVA, LC3 proteins; ASC PYCARD; Glutathione; TLR4; PI3K and NF-Kβ. That way, we verified that AuNP has greater affinity with the OVA molecule, followed by Glutathione and ASC PYCARD domain, whereas AuNP + OVA has greater affinity with the Glutathione molecule, followed by NF-Kβ. These results suggest that our AuNPs and AUNPs + OVA may be interfering in cell autophagy pathways, AuNPs in inflammatory and apoptotic cell pathways and AUNPs + OVA in inflammatory pathways. However investigation is needed to confirm and understand these trials.

BANKING MEMBERS:
Presidente - 2104326 - RAQUEL ALVES COSTA
Interno - 592.621.506-44 - CLAUDIA ROCHA CARVALHO - UFMG
Interna - 027.818.286-02 - ETEL ROCHA VIEIRA - UFVJM
Interna - 2279745 - ERIKA LORENA FONSECA COSTA DE ALVARENGA
Externo à Instituição - THIAGO CANTARUTI ANSELMO
Externa à Instituição - ALICE FREITAS VERSIANI