Banca de DEFESA: JANAINA DOMINGAS ALVES

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : JANAINA DOMINGAS ALVES
DATE: 04/03/2024
TIME: 08:30
LOCAL: On-line
TITLE:

Assessment of acute and subchronic toxicity of two recently developed β-carbonyls
synthesized

KEY WORDS:

acute and subchronic toxicity, β-carboline alkaloid, oncological treatments.

PAGES: 78
BIG AREA: Ciências Biológicas
AREA: Morfologia
SUBÁREA: Embriologia
SUMMARY:

Alkaloids are intriguing molecules, the simplest of which have chemical peculiarities and can
be added to the list of molecules with high pharmaceutical potential. Research carried out
with the aim of better understanding these compounds has enabled the development of
drugs associated to a wide range of therapeutic targets, being used to treat migraines,
headaches, depression, anxiety, neurological block, vascular and cardiac diseases, bacterial
infections, cancer, among others. In this sense, the research group “Núcleo de Pesquisa em
Química Biológica (NQBio), Nesse sentido, o grupo de pesquisa Núcleo de Pesquisa em
Química Biológica (Biosynthesized a series of unnatural β-carboline alkaloid analogs from
tryptamine and L-tryptophan. According to the cytotoxicity and selectivity against human
tumor cells in vitro, two of the alkaloids (NQBio-006 and NQBio-021) were taken to carry out
the present study. The aim of this study was to evaluate the acute and subchronic toxicity of
the compounds NQBio-006 e NQBio-021 in vivo. For this, female Swiss mice (n=80) with 8
weeks of age were usd. Animals were divided into 3 groups (Control n=12; NQBio-006 n=24
and NQBio-021 n=24) to the acute toxicity model establishment, in which a single dose of
2,000, 300, 50 and 5 mg/kg of both compounds were administered by gavage. For the
subchronic toxicity test, according to the best response observed in the acute toxicity, only
the NQBio-006 was administered at doses of 1,000, 100 and 10 mg/kg during a period of 28
days, for this test the animals were divided into 2 experimental groups (Control n=5 and
NQBio-006 n= 15). Signals of toxicity were observed at 5, 10, 20, 30 and 6 minutes as well
as 4, 8, 24 and 48 hours and during 14 days from the administration day for the acute study
and for 28 days for the subchronic evaluation toxicity. Data related to body and organ weight
and parameters associated with toxicity, such as erythrogram and platelet count,
histopathology and morphometry of the kidneys and liver, ovarian weight and follicular
population were obtained and analyzed using the D’Agostino-Pearson and t test through of
Prism 8.0. software. In relation to the acute toxicity test, significant differences were
observed in all the evaluated parameters, where the NQBio-021 demonstrated greater
toxicity. Therefore, the NQBio-006 were used to perform the subchronic toxicity tests, with
promising results being evidenced by the analysis, so that in the subchronic study, results
did not demonstrate significant differences. In general, it was possible to conclude that the
compound NQBio-006 has low toxicity and represents an important drug as a basis of
research related to oncological treatments.

BANKING MEMBERS:
Externa à Instituição - ANA BEATRIZ GRAÇA DUARTE
Externo ao Programa - 1680474 - FABIO VIEIRA DOS SANTOS
Presidente - 2141553 - PAULO HENRIQUE DE ALMEIDA CAMPOS JUNIOR