Banca de DEFESA: EMANUEL VICTOR BASTOS DA FONSECA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : EMANUEL VICTOR BASTOS DA FONSECA
DATE: 01/03/2024
TIME: 13:30
LOCAL: On-line
TITLE:

DEVELOPMENT OF A BIOMATERIAL BASED ON NATURAL POLYMERS ASSOCIATED WITH TITANIUM OXIDE (THIO2) NANOTUBES FOR BONE REPAIR

KEY WORDS:

Enxertos ósseos; matriz extracelular; biomateriais; polímeros

PAGES: 76
BIG AREA: Ciências Biológicas
AREA: Morfologia
SUMMARY:

Bone grafts that mimic the properties of the extracellular matrix (ECM) are well-
known for their ability to facilitate bone regeneration. Biomaterials can be designed

with a porous structure that mimics the natural architecture of bone tissue,
promoting cellular migration and differentiation. The development of biomaterials
often involves combining polymers and incorporating nanoparticles to create
composites with superior properties compared to individual components. In the
pursuit of enhanced composites, this study focused on developing collagen
scaffolds (2 mg) with varying amounts of alginate (1 mg, 2 mg, and 4 mg),
combined with three concentrations of TiO2 nanoparticles (2 μg, 4 μg, and 6 μg).
The scaffolds were prepared using type I collagen and alginate cross-linked with
CaCl2 through the casting and lyophilization technique, resulting in cylindrical
structures approximately 10 mm in length. FTIR analyses revealed the integrity of
collagen molecules, with slight shifts in the amide I and II groups due to
interactions with the C=O groups of alginate. AFM-QNM tests highlighted an
increase in the Young's Modulus (YM) in proportions equivalent to alginate and
collagen, with the 2:2 - 2 μg group achieving the highest YM at 3.52 ± 0.095. In this
proportion, the average pore size was 24.6 ± 2.10 μg, and the degradation rate

was 96% (2:2 - 4 μg) and 97% (2:2 – 6 μg). In vitro assays utilized human adipose-
derived mesenchymal stem cells (hASCs), demonstrating viability across all

scaffolds. The 4:2 proportion, with higher concentrations of alginate, stood out with
the best results by the end of the 3rd day. However, mineralization assessments
indicated lower mineralization in the 4:2 groups, while once again, the 2:2 groups
showed greater mineralized areas. The outcomes of this study contribute to
advancing the development of biomimetic scaffolds for bone regeneration. These
findings establish a solid foundation for future research and clinical applications in
the fields of tissue engineering and regenerative medicine.

BANKING MEMBERS:
Externo à Instituição - ALEXANDRE ANTUNES RIBEIRO - INT
Presidente - 2279745 - ERIKA LORENA FONSECA COSTA DE ALVARENGA
Externa à Instituição - MICHELE MUNK PEREIRA
Interna - 2104326 - RAQUEL ALVES COSTA